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Preparation method of test strip for morphine detection and application thereof

A technology of test strips and morphine, which is applied in the preparation of test strips for morphine detection and its application fields, can solve the problems of high price, experimental interference, and long detection cycle, and achieve high accuracy, strong specificity, and sensitivity high effect

Active Publication Date: 2014-01-15
广东省药品检验所
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Problems solved by technology

Although these methods have effectively strengthened the supervision of food and drugs, each has advantages but has obvious defects. Instrumental analysis such as liquid phase, gas phase, and liquid chromatography-mass spectrometry is expensive, requires a specific laboratory, and has a long detection cycle. Generally, it takes one day and requires the operation of professionals; the operation of thin-layer chromatography is complicated, and because the tested samples often contain a variety of spices, it will seriously interfere with the experiment and may obtain inaccurate results.

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  • Preparation method of test strip for morphine detection and application thereof
  • Preparation method of test strip for morphine detection and application thereof
  • Preparation method of test strip for morphine detection and application thereof

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Embodiment Construction

[0018] In order to facilitate a further understanding of the present invention, the present invention will now be described in detail in conjunction with specific embodiments. A test strip preparation method for morphine detection, comprising the steps of:

[0019] 1. Preparation of colloidal gold-labeled anti-morphine monoclonal antibody

[0020] The average size of colloidal gold particles is 30nm, add 1% trisodium citrate 1ml in 100ml deionized water, add 1% chloroauric acid 1ml quickly after boiling, continue to boil for 5min, after cooling, get colloidal gold solution, store at 4°C spare.

[0021] Take 100ml of the prepared colloidal gold solution and adjust the pH to 8.0 with 0.2mol / L sodium carbonate solution. While stirring, 1.5 mg of anti-morphine monoclonal antibody was added, stirred for 30 minutes, then 10 ml of 10% BSA was added dropwise, and stirred for 15 minutes. Centrifuge at 12000G for 15 min, discard the supernatant, add 10 ml of pH7.4 PBS buffer (contain...

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Abstract

The invention discloses a preparation method of a test strip for morphine detection and an application thereof. The preparation method comprises the following steps of: preparing a colloidal gold labeling anti-morphine monoclonal antibody, and coating the antibody on a colloidal gold combining pad; preparing a conjugate of morphine and BSA; spraying the conjugate and goat anti-mouse IgG on a nitrocellulose film as a detection line and a control line respectively, and drying; and assembling the test strip. The test strip for morphine detection disclosed by the invention has strong specificity, high sensitivity and high accuracy, does not need special equipment and is suitable for field operation; and moreover, the test strip can be quickly known well by an operator without professional knowledge, is very suitable for the daily supervision of the supervision law-enforcing department, can meet the needs for supervising inspection of basic level, and is widely used for detecting foods and medicines.

Description

technical field [0001] The invention relates to a preparation method of a test strip for morphine detection, and also relates to an application of the test strip for morphine detection. Background technique [0002] Morphine is an opioid alkaloid extracted from opium. It is a pure opioid receptor agonist and has a strong analgesic effect. Therefore, in medicine, morphine is a narcotic analgesic. This kind of substance has euphoria at the beginning of use, can't concentrate, will produce dream phenomenon, excessive use can cause acute poisoning, and it is very easy to produce tolerance and addiction. [0003] At present, relatively mature detection methods such as thin-layer chromatography (LC), gas chromatography (GC), liquid chromatography (HPLC), and liquid chromatography-mass spectrometry are used to detect morphine in food and drugs. Although these methods have effectively strengthened the supervision of food and drugs, each has advantages but has obvious defects. Instr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/577
Inventor 刘敏敏李延志张朝正温家欣陈俏余靖衡
Owner 广东省药品检验所
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