31results about How to "Fast reaction kinetics" patented technology

The invention provides an enriching method of circulating tumor cells (CTCs) and a kit thereof. The enriching method is applied to quickly and efficiently enriching CTCs from blood. The enriching method comprises the following steps of coupling an anti-epithelial cell adhesion molecule (EpCAM) antibody onto a magnetic nanosphere to prepare an immune-magnetic bead; adding the immune-magnetic bead into a blood sample for hatching for 5 minutes, and realizing separating, enriching and purifying of CTCs by the steps such as magnetic separating and washing. The enriching method disclosed by the invention is high in capturing efficiency, short in enriching time, good in specificity and very reliable. Activity ratio of the captured cell is kept at (90.5 +/-1.2)%, and the captured cell can be directly used for cultivation, reverse transcriptase polymerase chain reaction (RT-PCR) and immune cell chemical analysis. Besides, the enriching method provides powerful means for detecting and researching CTCs, and has extremely important medical significance.

Methods and devices for powdered sorbent regeneration of biologic fluids are disclosed. The present invention includes three novel methods, which may be used singly or in any combination, for constraining or immobilizing powders so that they can be perfused with a biological fluid or dialysate: a porous carbon block filter, a filtration bed of very fine powder, and a cone-shaped reactor.

The invention discloses a preparation method of a copper-containing metal composite oxide photocatalytic material. The preparation method comprises the following steps: putting copper and zirconium into a vacuum electric arc furnace, performing vacuum pumping, then charging argon, starting smelting, keeping for 60-90 seconds after copper and zirconium are smelted, cooling to obtain an alloy molten ingot, turning over, repeating for 4 to 6 times, putting the alloy molten ingot into rapid melt quenching and melt spinning equipment, performing vacuum pumping, then charging argon, performing smelting by adopting a magnetic induction heating mode to form a melt after completely melting, opening an air pressure valve, spraying the melt on the surface of a copper roller which rapidly rotates to obtain a continuous amorphous alloy ribbon, and putting the amorphous alloy ribbon into a ceramic cup, wherein the ceramic cup comprises a ceramic base, the ceramic base is connected with a ceramic cup cover, the ceramic cup cover is provided with an air inlet hole, an observation view window and a graphite electrode, and the ceramic base is in sliding connection with an ignition graphite electrode; and connecting one end of the amorphous alloy ribbon with the graphite electrode, charging oxygen, then pushing the ignition graphite electrode to the amorphous alloy ribbon, and enabling the ignition graphite electrode to be in contact with the amorphous alloy ribbon to generate electric sparks for igniting an alloy strip material.

The invention relates to a preparation method of mesoporous nitrogen-doped graphene-loaded molybdenum disulfide synthesized by laser irradiation and application of the mesoporous nitrogen-doped graphene-loaded molybdenum disulfide in electrocatalytic hydrogen production. In order to solve the problems that by an existing synthesis process, transition metal oxide/sulfide composite mesoporous nitrogen-doped graphene rich in carbon-pyridine nitrogen metal bonds cannot be synthesized at low temperature and under low pressure, and the content of the carbon-pyridine nitrogen metal bonds in the composite system cannot be regulated and controlled effectively, it is found that the content of the carbon-pyridine nitrogen-molybdenum bonds in the composite catalyst can be improved by irradiating graphene oxide with laser in a range of 177-315 mJ, and in a hydrothermal process, the mass ratio of laser irradiation graphene oxide to tetrathiomolybdic acid as raw materials is 1: 1-1: 8, the loading amount of the molybdenum disulfide on mesoporous graphene can be optimized, thus, while the conductivity of the molybdenum disulfide is improved, the intrinsic activity of the molybdenum disulfide can also be improved synergistically by the carbon-pyridine nitrogen molybdenum bonds at an interface, and the electrocatalysis process of HER is promoted. The preparation method is simple in process, ingenious in design and low in cost, and is safe and environmentally friendly.

The invention belongs to the related technical field of metal heat treatment, and discloses a high-temperature adjacent metal heat treatment device and method. The device comprises an upper polar plate and a lower polar plate which are oppositely arranged, wherein the upper polar plate and the lower polar plate are both made of Joule thermal materials; a to-be-treated workpiece is placed between the upper polar plate and the lower polar plate; after current is introduced into the upper polar plate and the lower polar plate, the upper polar plate and the lower polar plate are rapidly heated, and the temperature is transmitted to a heat treatment part; and after the current is cut off, the heat treatment part is cooled. the upper polar plate and the lower polar plate are quickly cooled, so that the temperature in the to-be-treated workpiece is reduced, and heat treatment of the to-be-treated workpiece is realized. In the heat treatment process, the processes of atomic diffusion, phase transformation, grain growth and the like all accord with the heat activation process, faster reaction kinetics can be provided, exponential shortening of the heat treatment time is achieved, the heat treatment method which is high in speed and low in energy consumption and can accurately control the performance of the needed metal material is provided, and the method is wide in applicability and good in batch treatment effect.

The invention discloses a hollow carbon shell inlaid with metal sulfide, a preparation method and application thereof, and belongs to the technical field of preparation of lithium-sulfur battery positive electrode materials, wherein a hollow carbon shell polyhedral material inlaid with polar metal sulfide is prepared by taking MOFs as a precursor and performing simple vulcanization and heat treatment. According to the invention, the preparation method is easy to operate, required instruments and equipment are simple, a ZIF-67 precursor template with uniform morphology is prepared by adopting a precipitation method with a mature process, and by using a simple vulcanization process, a hollow structure can be formed due to non-uniform shrinkage in a heat treatment process; and due to the fact that chemical adsorption can be generated on polysulfide, the shuttle effect in the charging and discharging process can be restrained to a certain degree, loss of the active substance sulfur is reduced, the hollow structure can buffer the volume change of sulfur in the electrochemical reaction process, so that the electrochemical performance of the battery can be improved, and the hollow carbon shell can be applied to positive electrode sulfur carriers of lithium-sulfur batteries.

The invention discloses a preparation method of a Fe2O3/C@Co2B catalyst and application of the Fe2O3/C@Co2B catalyst in an oxygen evolution reaction. According to the invention, Fe2O3/C is prepared byusing a liquid plasma arc discharge method; The Fe2O3/C@Co2B catalyst with a core-shell structure is prepared by using a chemical reduction method. After being washed and dried, a catalyst sample isapplied to the process of an electrocatalytic oxygen evolution reaction. Results show that under a current density of 10 mA/cm<2>, the overpotential of the Fe2O3/C@Co2B catalyst is only 332 mV, a reduction of 33 mV compared with the overpotential (365 mV) of a commercial RuO2 catalyst. Furthermore, the Tafel slopes of Fe2O3/C@Co2B and RuO2 are 48 mV/dec and 68 mV/dec respectively, so compared withcommercial RuO2, Fe2O3/C@Co2B has a smaller Tafel slope, which indicates that Fe2O3/C@Co2B has faster reaction kinetics. Moreover, the stability of Fe2O3/C@Co2B is obviously improved compared with the stability of Co2B; and Fe2O3/C@Co2B can stably catalyze water electrolysis for 12 hours without obvious reduction, and the overpotential of Fe2O3/C@Co2B is not obviously increased after 2000 CV cycles.

The invention relates to the field of a fuel cell electrolyte and discloses a proton conductor material, a preparation method and application thereof, and an intermediate temperature fuel cell. The chemical formula of the proton conductor material is (NH4)2A1-xBxP4O13, wherein A is one, two or more than two elements of Y, Ti, Nb, Ta, Mo and W; B is one, two or more than two elements of Al, In, Si, Ge and Sn; and x is greater than or equal to 0 and less than or equal to 0.4. When the proton conductor material provided by the invention works in an intermediate temperature (150 to 400 DEG C) range, the proton conductivity is obviously higher than 4*10<-2>S/cm, and application of the proton conductor material to the intermediate temperature fuel cell can be realized.

The invention discloses a peroxidase catalyzed cell surface protein labeling method. Exogenous peroxidase is introduced outside a cell to catalyze a chemical probe to generate free radicals with high activity and a short service life to selectively and covalently label protein groups exposed on the outer side of a cytoplasmic membrane. After reacting for a period of time, a sealing buffer solution is added to terminate the labeling reaction; and then, proteomic analysis is performed on the labeled protein by using a capturing group on the chemical probe. The free radical of the chemical probe can be covalently linked with one or more than two of amino acids with higher electron density, such as tyrosine, tryptophan, histidine, cysteine and the like, on an adjacent protein. The labeling strategy is simple to operate, good in selectivity and high in reaction rate, does not damage glycoforms of cell surface proteins, can be applied to analysis of cell surface proteome (or plasma membrane proteome) and a dynamic change thereof, and can also be applied to research of a cell surface protein-protein interaction, a cell-cell interaction and the like.