Enriching method of circulating tumor cells
A tumor cell enrichment technology, applied in the field of nanomaterials, can solve the problems of cumbersome operation, slow magnetic response, inconvenient for ordinary users, etc., and achieve good enrichment effect, good reproducibility, fast and efficient capture and enrichment Effect
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[0024] Example 1 Enrichment and analysis of circulating tumor cells
[0025] Taking the breast cancer cell SK-BR-3 as an example, the use of magnetic nanospheres to efficiently and quickly capture and sensitively detect circulating tumor cells will be described in detail.
[0026] 1. Method
[0027] 1. Preparation of Magnetic Nanospheres
[0028] The preparation of magnetic nanospheres is based on the preparation of fluorescent-magnetic double-coded spheres in our laboratory, that is, the assembly method is used to assemble magnetic nanoparticles nano-γ-Fe on the surface of styrene-propionamide nanospheres. 2 O 3 . Specifically: ①The styrene-acrylamide copolymerized nanospheres were prepared by the emulsion-free polymerization method, and the carboxyl groups were modified on the surface of the nanospheres by hydrazation and succinic anhydride, and the obtained by EDC / NHS activation method A layer of polyethyleneimine (PEI) is assembled on the surface of the carboxyl nanospheres, an...
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