63 results about "Chemical probe" patented technology

A method of fabricating an addressable array of chemical probes at respective feature locations on a substrate surface. The method may use a deposition apparatus with a substrate unit which includes the substrate and with a drop deposition unit which includes a drop deposition head. Such an apparatus when operated according to a target drive pattern based on nominal operating parameters of the apparatus provides the probes on the substrate surface in the target array pattern. The method may include depositing at least one drop from the head unit onto the substrate surface. A fiducial on the substrate unit is optionally viewed from a sensor. A deposited drop on the substrate surface is viewed from a sensor. An actual position of the viewed deposited drop may be determined relative to a fiducial on the substrate unit, based on the views of the fiducial and deposited drop. An error is determined based on any difference between the actual and target positions. Alternatively, an error for one or more deposition units may be determined based on a statistical difference between the actual and target positions of a set of multiple drops deposited from each deposition unit. The deposition apparatus is operated to deposit further drops from the head unit onto the substrate surface at feature locations while moving at least one of the substrate unit or head unit with respect to the other, so as to fabricate the array. Apparatus and computer program products are also provided.

The invention relates to a preparation method of a dopamine composite nano-fiber affinity membrane for adsorbing La3+. The preparation method comprises the steps of (1) dissolving polymer materials in a solvent to obtain a polymer blended solution with the mass percentage concentration of 8%-30%, and then obtaining a composite nano-fiber membrane through electrostatic spinning, and (2) soaking the composite nano-fiber membrane in a Tris-HCl solution of dopamine, and obtaining the dopamine composite nano-fiber affinity membrane for adsorbing the rare-earth metal ion La3+. The preparation method is simple and practical, raw materials can be easily obtained, the cost is low, the dopamine composite nano-fiber affinity membrane for adsorbing the La3+ can be conveniently and accurately prepared, and the large-scale production operation can be easily achieved. According to the preparation method, the dopamine composite nano-fiber affinity membrane with the high specific surface area can be widely applied to the fields such as rare-earth metal La3+ gathering, rare-earth metal La3+ adsorbing, rare-earth metal La3+ separation, chemical probes, sensors and water treatment.

The present disclosure relates to a method for metabolic oligosaccharide engineering that incorporates derivatized alkyne-bearing sugar analogs as “tags” into cellular glycoconjugates. The disclosed method incorporates alkynyl derivatized Fuc and alkynyl derivatized ManNAc sugars into a cellular glycoconjugate. A chemical probe comprising an azide group and a visual probe or a fluorogenic probe is used to label the alkyne-derivatized sugar-tagged glycoconjugate. In one aspect, the chemical probe binds covalently to the alkynyl group by Cu(I)-catalyzed [3+2] azide-alkyne cycloaddition and is visualized at the cell surface, intracellularly, or in a cellular extract. The labeled glycoconjugate is capable of detection by flow cytometry, SDS-PAGE, Western blot, ELISA or confocal microscopy, and mass spectrometry.

The present disclosure relates to a method for metabolic oligosaccharide engineering that incorporates derivatized alkyne-bearing sugar analogs as “tags” into cellular glycoconjugates. The disclosed method incorporates alkynyl derivatized Fuc and alkynyl derivatized ManNAc sugars into a cellular glycoconjugate. A chemical probe comprising an azide group and a visual probe or a fluorogenic probe is used to label the alkyne-derivatized sugar-tagged glycoconjugate. In one aspect, the chemical probe binds covalently to the alkynyl group by Cu(I)-catalyzed [3+2] azide-alkyne cycloaddition and is visualized at the cell surface, intracellularly, or in a cellular extract. The labeled glycoconjugate is capable of detection by flow cytometry, SDS-PAGE, Western blot, ELISA or confocal microscopy, and mass spectrometry.

A biological or chemical optical sensing device comprises at least one planar micro-resonator structure included in a light emitting waveguide, and at least one biological or chemical probe bound to at least a part of the micro-resonator structure, the probe operative to bind specifically and selectively to a respective target substance, whereby the specific and selective binding results in a parameter change in light emitted from the waveguide. In one embodiment, the micro-resonator is linear. In some embodiments, the sensing device is active, the waveguide including at least one photoluminescent material operative to be remotely pumped by a remote optical source, and the parameter change, which may include a spectral change or a quality-factor change, may be remotely read by an optical reader.

Chemical stain compounds containing a fluorophore and a reducible quenching unit are disclosed. The reducible quenching unit quenches the fluorophore while in its oxidized state. Upon reduction, the quenching properties of the quenching unit are diminished or eliminated. The chemical compounds can be used in a variety of applications, including the detection of bacterial cells, monitoring the electron transport chain function of bacterial cells, monitoring the oxidation state of non-biological systems, and assaying the effectiveness of antibacterial or antimicrobial agents.

A method for control data quality of automatic buoy monitoring system relates to an automatic buoy monitoring system. Data quality analysis, before data quality control, needs to analyze the data quality. Data quality analysis is an important link in the data preparation process of buoy data characteristic analysis, and is the prerequisite of data pretreatment. Outlier elimination method, aiming at the outlier obtained by the automatic monitoring system; on-site validation and evaluation: validate and evaluate the monitoring parameters of the buoy through on-site sampling and laboratory measurement, especially the data measured by biological and chemical probes, to ensure the reliability of the data; The comparison parameters mainly include NO3, DO, Chl and CDOM. Water samples can be collected at the place where the buoys are placed and brought back to the laboratory for analysis; then the correlation curve between the laboratory analysis data and the buoy data is drawn to judge the reliability of the buoy data from its distribution trend.

A method and apparatus for probing the chemistry of a single droplet are provided. The technique uses a variation of the field-induced droplet ionization (FIDI) method, in which isolated droplets undergo heterogeneous reactions between solution phase analytes and gas-phase species. Following a specified reaction time, the application of a high electric field induces FIDI in the droplet, generating fine jets of highly charged progeny droplets that can then be characterized. Sampling over a range of delay times following exposure of the droplet to gas phase reactants, the spectra yield the temporal variation of reactant and product concentrations. Following the initial mass spectrometry studies, we developed an experiment to explore the parameter space associated with FIDI in an attempt to better understand and control the technique. In an alternative embodiment of the invention switched electric fields are integrated with the technique to allow for time-resolved studies of the droplet distortion, jetting, and charged progeny droplet formation associated with FIDI.

A metal oxide supported palladium catalyst comprised of a β-Bi2O3 / Bi2Sn2O7 hetero-junction catalyst support and palladium was developed. The catalyst was synthesized using a sol-gel technique as a nanocrystalline structure. In the presence of fluorene, an oxidant and ultraviolet irradiation, the catalyst converts the hydrocarbon to a mixture of fluorenol / fluorenone oxidation products. The close proximity between β-Bi2O3 and Bi2Sn2O7 heterojunction phases in the catalyst is thought to be responsible for the efficient charge separation and catalytic activity. An indirect chemical probe method using active species scavengers elucidated that the photo-oxidation mechanism proceeds via holes and superoxide radical (O2.−) moieties.

Chemical stain compounds containing a fluorophore and a reducible quenching unit are disclosed. The reducible quenching unit quenches the fluorophore while in its oxidized state. Upon reduction, the quenching properties of the quenching unit are diminished or eliminated. The chemical compounds can be used in a variety of applications, including the detection of bacterial cells, monitoring the electron transport chain function of bacterial cells, monitoring the oxidation state of non-biological systems, and assaying the effectiveness of antibacterial or antimicrobial agents.

The present invention discloses a water-soluble cationic conjugated microporous polymer phosphorescent probe and a preparation method thereof. 2-(4-bromophenyl) pyridine iridium dichloride bridged complex Ir2(Brppy)4Cl2, 3,8-dibromo phenanthroline and 3,8-dibromo phenanthroline iridium 2-(4-bromophenyl) complex are sequentially prepared to prepare an objective polymer. The polymer chemical probe prepared through the above technical scheme has good water solubility and can bind with nucleic acid through electrostatic interaction; the probe has good phosphorescence emission in water and long luminescence lifetime; and a time-resolved fluorescence technique can effectively eliminate interference of the background fluorescence and improve detection sensitivity. The preparation preparation method has the advantages of simple operation and easily available raw materials, and is beneficial to reduce the cost.

The invention relates to a preparation method of an aminated nanofiber membrane with a high specific surface area. The preparation method comprises the following steps of: dissolving two polymer materials with the weight ratio of 1: (0.1-10) or dissolving a polymer material and an inorganic salt into a solvent, dissolving while stirring at the temperature of 20-80 DEG C for 4-96h, and carrying out electrostatic spinning to obtain a composite nanofiber membrane; soaking the composite nanofiber membrane into water, regulating the pH value, soaking at the temperature of 20-95 DEG C for 2-48h, washing and drying to obtain a nanofiber membrane with a porous micro / nano structure and a high specific surface area; and immersing the nanofiber membrane with a porous micro / nano structure and a high specific surface area into water, adding an amination reaction reagent to carry out amination reaction at the temperature of 50-200 DEG C, then, taking out the fiber membrane, washing the fiber membrane to be neutral, and drying the fiber membrane to obtain the aminated nanofiber membrane with a high specific surface area. The product provided by the invention is applied to fields such as adsorption and separation of precious metal ions, heavy metal ions and transition metal ions, chemical probes, sensors, environment monitoring, catalysts, biological medicines and the like.

The invention relates to a preparation method of a disulfide dot / nano sheet composite DNA electrochemical probe. The method specifically includes the following steps: 1) ultrasonically stripping the transition metal disulfide powder, high-temperature electromagnetic stirring to prepare a transition metal disulfide quantum dot / nanosheet composite, ultrasonically in a dispersion of thionine and an ionic liquid, Prepare a thionine functionalized complex; 2) Disperse the prepared complex in an organic solvent, make a suspension and drop-coat it on the electrode, and obtain a complex modified electrode after drying; 3) Prepare the above prepared electrode In the three-electrode system, the electrochemical test is performed in the buffer solution, and DNA solutions of different concentrations are injected for testing, and the electrochemical signal changes on the electrode surface are recorded to construct a DNA electrochemical probe system. The probe of the invention has the advantages of high sensitivity, good stability, wide linear range, low detection limit, etc., can be used for detecting DNA in serum, and has broad application prospects in the fields of biological analysis and clinical diagnosis.

The present invention provides a method of optical electrophysiological probing, including: providing a fluorescing chemical probe; contacting a thick portion of tissue with the fluorescing chemical probe to create a thick portion of treated tissue; applying a first range of wavelengths of electromagnetic radiation to the treated portion of tissue; and detecting a plurality of depth-specific emission wavelengths emitted from the thick portion of treated tissue.