51 results about "Chemical probe" patented technology

Disclosed herein are chemical probes for unfolded, misfolded or intrinsically disordered proteins, in particular chemical probes having the structure of Formula I or salts thereof, and to the use of the probes for various applications relating to unfolded, misfolded or intrinsically disordered proteins, for example, for diagnosing or prognosing a disease or disorder associated with an unfolded, misfolded or intrinsically disordered protein.

Affinity-based and activity-based probes (ABPs) described herein offer transformative resolutions to microbiome function. These ABPs target key metabolic pathways in the gut microbiome. The ABPs contain a binding group, a reactive group, and a. reporter group handle that allows for the addition of a "flexible" reporter group that can be easily swapped to enable multimodal fluorescence and proteomic measurements and isolation of live cells. The binding group, also called an affinity element or biorecognition element, mirrors monomeric and polymeric carbohydrates, sulfated and acetylated carbohydrates, and peptides to afford probe selectivity.

The application relates to the technical field of free radical detection, in particular to a high-sensitivity in-situ capture and detection method of organic free radicals based on a DIPPMPO probe and application, and the method comprises the following steps: adding a chemical probe DIPPMPO into a reaction system containing potential organic free radical precursors, so that the DIPPMPO and organic free radicals generated in a reaction process occur adduct reaction to form a DIPPMPO-free radical adduct which can be detected under liquid chromatography-mass spectrometry conditions; performing high performance liquid chromatography-quadrupole-time of flight mass spectrometry analysis on a mixed solution after the adduct reaction is completed, so that first mass spectrometry data and secondary mass spectrometry fragment information of the DIPPMPO-free radical adduct are obtained; and automatically processing the secondary mass spectrometry data, screening out parent ions with continuous neutral loss as potential DIPPMPO-free radical adduct candidate signals. The application does not need derivatization, is simple to operate, is broad-spectrum and high-efficiency, and can be applied to environmental water samples, biological fluids, chemical reaction liquids or drug metabolism systems.

A tetrafunctional compound, which is formed by connecting a ligand binding site (A) or a ligand, a reactive site (D), a potential cleavable site (E) and a biotin site (B) through a spacer as needed, wherein: the ligand binding site (A) is an activated functional group such as an amine reactive group or a reactive functional group such as -COOH; the reactive site (D) is a group having a structure of a compound such as 2-aryl-5-carboxytetrazolyl; the potential cleavable site (E) is a group having a structure of a compound such as 1-(4,4-dimethyl-2,6-dioxocyclohexyl-1-ylidene)ethyl; and the spacer is a group obtained by substitution with a cross-linking group, such as a straight or branched alkylene group having one or more carbon atoms.

The application discloses a polymer probe and a preparation method and application thereof, and belongs to the technical field of chemical probes.The structural formula of the polymer probe provided by the application is shown as formula (I): in the formula (I), x and y are each independently selected from any integer in 1-3000.The polymer probe has good water solubility, obvious visual color change for sulfur dioxide and derivatives thereof, and can be used for qualitative or quantitative detection of sulfur dioxide and derivatives thereof in water; meanwhile, the polymer probe has thermal response performance, can be recycled and reused by heating after a reaction of the polymer probe with sulfur dioxide and derivatives thereof in an aqueous solution is completed, is used for secondary analysis and identification of sulfur dioxide and derivatives thereof, has good reusability, is favorable for cost reduction, and has wide application in detection of sulfur dioxide and derivatives thereof.

Provided herein are methods for detecting the presence or progression of a disease in a subject as well as a method for determining the efficacy of a therapy for the disease by administering to the subject a probe comprising a phosphorothioated DNA barcode sequence, wherein the DNA barcode sequence comprises about 36 nucleotides to about 72 nucleotides, and wherein the DNA barcode is released when the disease is present in the subject; obtaining a biological sample containing the released DNA barcode from the subject; and amplifying and detecting the DNA barcode in the biological sample.

The application discloses a kind of based on high-flux array spectrum and deep learning multivariate antibiotic rapid detection method, belong to water environment new pollutant detection technical field.The application includes the following steps: antibiotic spectrum sensing system construction, combinatorial chemistry probe screening, high-flux spectrum experiment, deep learning chemometric model development, antibiotic intelligent detection system development.The high-flux combinatorial array spectrum sensing technology used in the application acquires fingerprint image data with holographic spectrum imaging as core, greatly improves experimental efficiency, expands sampling range by combining high-flux experiment and machine learning model, can cover more kinds of chemical mixture combination, deep learning model is good at extracting complex features from a large amount of data, can accurately identify and distinguish the fingerprint spectrum of different kinds of antibiotics, even in complex water environment also can maintain high sensitivity and high specificity, improve the accuracy and response speed of pollutant detection in complex chemical system.

A tetra-functional compound contains a ligand-binding moiety (A) or ligand, a reactive moiety (D), a cleavable moiety (E), and a biotin tag (B), which are linked optionally via a spacer, wherein the ligand-binding moiety (A) is an activated functional group such as an amine-reactive group, or a reactive functional group such as —COOH; the reactive moiety (D) is a group having the structure of a compound such as 2-aryl-5-carboxytetrazole; the cleavable moiety (E) is a group having the structure of a compound such as 1-(4,4-dimethyl-2,6-dioxocyclohex-1-ylidene)ethyl; and the spacer is a group such as a linear or branched alkylene group having one or more carbon atoms, substituted with a crosslinking group.

Aspects of the disclosure relate to chemical probes for the detection of proteins of interest. The chemical probes can be employed in activity-dependent proximity ligation methods for quantifying proteins of interest in single cells. In some embodiments, the chemical probes can be employed in identifying enzymes in single cells.

The invention belongs to the field of pharmaceutical technology and chemical probe development, and discloses preparation of a perylene bisimide derivative and application of the perylene bisimide derivative as a mitochondrial transcription inhibitor, the structural formula of the non-planar perylene bisimide derivative meets the general formula (I) or (II), in the general formulas (I) and (II), four R1 groups are selected from H or halogen atoms, and at least one R1 group is a halogen atom. It is proved for the first time that the compounds specifically inhibit mitochondrial gene transcription in tumor cells and play an anti-tumor role. Meanwhile, due to the fluorescence characteristic of the perylene bisimide derivative, the prepared compound can also be used for fluorescence imaging of mitochondria, compared with an existing mitochondria probe, the mitochondria probe with a novel structure is provided, a new structural selection is provided for a mitochondria-targeting fluorescent dye, and the application prospect is wide. And a reliable tool is provided for the application of cell mitochondria imaging and anti-tumor treatment.

The application discloses a crotonylation detection probe and a preparation method and application thereof, and the preparation method comprises the following steps: taking an alkyne alcohol (compound II) as raw material, reacting with methoxycarbonylmethylidene triphenyl positive phosphorus under the action of an oxidant to obtain a methyl ester compound (compound III), hydrolyzing the methyl ester compound (compound III) under the action of alkali to obtain an acid (compound IV), and finally reacting the acid (compound IV) with alkali, filtering and freeze-drying to obtain the probe molecule (compound I). The chemical probe (compound I) with an alkyne group is applied in cells, the alkyne group is selectively loaded on target proteins at specific modification sites through metabolic labeling, and through a bio-orthogonal chemical reaction, a fluorescent dye can be orthogonally connected to the modification site, so that the probe is used for fluorescent imaging of crotonylation proteins in cells and identification of crotonylation modification substrates and sites, and by using the probe, crotonylation sites regulated by histone deacetylases (HDACs) can be determined through quantitative chemical proteomics.

This invention discloses a Pt-Ni@erGO electrochemical probe, its preparation method, and its applications. The Pt-Ni@erGO electrochemical probe comprises an erGO matrix and Pt-Ni nanoparticles supported on the erGO matrix. The Pt-Ni@erGO electrochemical probe of this invention uses erGO as a matrix to grow Pt-Ni alloy nanoparticles in situ. erGO simultaneously exhibits two functions to enhance electrochemical detection sensitivity: firstly, it acts as a carrier to load high-density Pt-Ni alloy nanoparticles, reducing the shielding effect of biorecognition elements on the catalytic sites of nanozymes; secondly, it acts as a biorecognition element to specifically bind to *E. coli*. Through the rational combination of erGO and Pt-Ni nanoparticles, the obtained Pt-Ni@erGO possesses high antibody-like activity and electrocatalytic activity.

This invention discloses a method for detecting the content of a target component in a test component based on redox potential, comprising the following steps: inserting a first electrode and a first reference electrode into a test component containing or not containing the target component; inserting a second electrode and a second reference electrode into an electrochemical probe solution, wherein the electrochemical probe solution is used to generate a chemical potential gradient between the first electrode and the second electrode; connecting the first electrode and the second electrode with a wire; measuring the open-circuit voltage between the first reference electrode and the second reference electrode; and determining the content of the target component according to a standard curve. This method, by rationally selecting the electrochemical probe to ensure that the reduction potential of the cathode is positive than the oxidation potential of the anolyte, allows the electrochemical process in the entire circuit to proceed spontaneously without the need for an external voltage, thereby avoiding the generation of oxidation products. It exhibits superior stability during long-term detection of the target component, especially for analytes such as 5-hydroxytryptamine that severely contaminate the electrode.

The present invention discloses an alkyl chain-modified metal organic iridium complex, a preparation method and uses thereof, and relates to the technical field of organic photoelectric functional materials. Compared with the prior art, the iridium complex prepared by the present invention not only has a novel chemical structure and a mature and reliable preparation route, but also can change the hydrophobicity of the molecule through modification of the alkyl chain, effectively regulating the luminescence performance of the molecule. The luminescence intensity of the iridium complex is high in a high-concentration solution or in an aggregated state, and therefore has broad application value in the fields of biological cell imaging, organic light-emitting diode devices, chemical probes, optical films, etc.

The application discloses a surface fluorescence chromatography resin embedding method suitable for immunostaining samples, and comprises the following steps: S1, biological tissue sample pretreatment; S2, gradient ethanol dehydration and tissue blackening; S3, gradient resin permeation; S4, acidification treatment; and S5, resin polymerization molding. The application also comprises a biological tissue sample imaging method, which comprises the step of imaging the biological tissue sample obtained by the embedding method in a fMOST system sodium carbonate solution environment. The application temporarily quenches the pH-sensitive chemical probe by adding HAc; in combination with the fMOST imaging system, the reactivation is realized in the alkaline solution environment, the blackening chemical chromatography high-precision imaging is realized by reactivating the sample imaging surface, and the immunostaining sample has the advantages of high fluorescence retention rate.

The invention relates to an oxazole compound and a preparation method and application thereof, the preparation method comprises the following steps: in an organic solvent, under the action of ferric chloride, elemental iodine and oxygen, an alpha-aminoketone hydrochloride compound and an aldehyde compound are subjected to an oxidative cyclization reaction, and after the reaction is finished, post-treatment is performed to obtain the oxazole compound, the oxazole compound is applied to preparation of fluorescent dyes, OLED materials, drugs, catalysts, functional polymers and chemical probes. The method has the advantages of high reaction yield and convenience in separation, and shows excellent environmental friendliness, simplicity, high efficiency, mildness and low-cost economy of a new process technology.

The invention belongs to the technical field of chemical probes, and particularly relates to a preparation method of a probe for analyzing intestinal microbiota to regulate BSH activity. The preparation method of the probe for analyzing intestinal microbiota to regulate BSH activity comprises the following steps: taking 7-amino-4-methyl-3-coumarin acetic acid (AMCA) as a raw material, sequentially carrying out a first reaction with a chlorinating agent and an alcohol reagent, carrying out a second reaction with a CA compound in the presence of a condensing agent and a catalyst, and carrying out a hydrolysis reaction to obtain the required CA-AMCA probe. According to the preparation method of the BSH probe, the raw materials are easy to obtain, the process route is simple, the total reaction yield reaches up to 64%, and the product yield can be effectively guaranteed.

The invention discloses a PROTAC compound for degrading TRIM15 protein. The PROTAC compound has a structure as shown in a formula (I). The molecule can effectively inhibit the RIG-1 signal channel, and has application prospects in preparation of anti-inflammatory or autoimmune disease drugs. Meanwhile, the invention further provides a chemical probe molecule with the structure shown in the formula (III). One end of the molecule is combined with TRIM15, and the other end of the molecule can be combined with tag protein (such as Halog-tag) or other target protein, so that TRIM15 is recruited as E3 ubiquitin ligase to carry out ubiquitination modification on the tag protein (or the target protein). The molecule can be used as an innovative research tool and is used for exploring the biological function of the E3 ligase of TRIM15, the substrate specificity and non-K48 type ubiquitination modification.

The present invention discloses a Schiff base iridium complex with aggregation-induced emission effect and its preparation and application, relating to the technical field of metal complex luminescent materials. The present invention designs and synthesizes a Schiff base iridium complex with a novel structure. Testing shows that the luminescence intensity of the Schiff base iridium complex in an aggregated state is significantly stronger than that of a low-concentration solution, and has a significant aggregation-induced emission effect. The complex has broad application prospects in technical fields such as biological cell imaging, organic light-emitting diode devices, chemical probes, and optical films.

The present application belongs to the technical field of chemical probes, and particularly relates to a preparation method of a probe for analyzing intestinal flora regulating BSH activity. The preparation method of the probe for analyzing intestinal flora regulating BSH activity provided by the present application uses 7-amino-4-methyl-3-coumarin acetic acid (AMCA) as raw material, sequentially carries out a first reaction with a chlorinating agent and an alcohol reagent, and carries out a second reaction with a CA compound in the presence of a condensing agent and a catalyst, and the required CA-AMCA probe can be obtained through a hydrolysis reaction. The preparation method of the BSH probe provided by the present application not only has easily obtained raw materials and a simple process route, but also has a total reaction yield as high as 64%, which can effectively guarantee the product yield.

The application relates to a silver-doped hollow carbon nanosphere electrochemical probe and a preparation method thereof, and belongs to the technical field of electroanalysis chemical detection and carbon nanomaterials. The Ag@HCS composite material is prepared by using a SiO2 template method, polymerizing formaldehyde and m-diphenol to form a phenolic resin ball in the presence of silver nitrate, and then performing carbonization treatment and in-situ reduction of silver and removing the template. The Ag@HCS is used for HbA 1c The determination of HbA 1c is realized based on the silver-based electrochemical oxidation signal of the electrochemical probe after the modification of the nucleic acid aptamer. Experiments show that the electrochemical sensor constructed by the method has high sensitivity, a wide detection range, high specificity and good stability, and exhibits good feasibility in the detection of actual samples, and the method provides a method for synthesizing a stable, controllable and sensitive electrochemical probe.

Provided herein are methods for identifying or differentiating an analyte, such as a bulk surface or a soil sample. The presently disclosed methods involve contact between an oligourethane solution and an analyte, wherein interactions between the oligourethane chemical probe solution and the analyte result in unique data patterns that can be interpreted to identify or differentiate the analyte.

Disclosed herein are chemical probes for unfolded, misfolded or intrinsically disordered proteins, in particular chemical probes having the structure of Formula I or salts thereof, and to the use of the probes for various applications relating to unfolded, misfolded or intrinsically disordered proteins, for example, for diagnosing or prognosing a disease or disorder associated with an unfolded, misfolded or intrinsically disordered protein.

The invention belongs to the technical field of protein detection, and particularly relates to a chemical probe, a preparation method of the chemical probe and application of the chemical probe in detection of Tau protein aggregates. The chemical probe can specifically recognize the Tau protein aggregate, and after the chemical probe is combined with the Tau protein aggregate, a fluorescence signal can be remarkably enhanced, so that the Tau protein aggregate is detected; and after long-term illumination, good characteristics can still be maintained, and the stability is good.

This disclosure relates to the field of anticancer drug technology, and discloses a 7-azaindole compound, its synthesis method, and its uses. The structure of the 7-azaindole compound is as follows: This 7-azaindole compound can be used as a fluorescent polarization probe or a degradation chemical probe targeting CSN5; wherein, the fluorescent polarization chemical probe has a good binding affinity to the metalloproteinase CSN5, laying an important foundation for the discovery of novel CSN5 inhibitors; at the same time, the degradation chemical probe exhibits good enzyme-level activity and cell-inhibitory activity, and has the potential to degrade CSN5 protein and its variants, providing a material basis for the development of related drugs targeting CSN5 and the study of its biological functions, and also has great potential in the discovery of cancer immunotherapy drugs and the treatment of other related diseases.

The present disclosure provides a detection method of wormlike micelles, and belongs to the technical field of analytical chemistry. In the present disclosure, z-ArN2+ is used as a chemical probe substance; a long-chain probe is added to a test solution for probing an interfacial region, and a short-chain probe is added to a surfactant-free control solution. A selectivity of the probe with different nucleophiles is determined by the above method, and a yield of an obtained product is measured by high-performance liquid chromatography (HPLC); and the wormlike micelles are detected by measuring the yield of the product using the selectivity of the short-chain probe to be converted into an interfacial mole number. The wormlike micelles are detected by chemical probes, and the chemical probes have a simple preparation method, a low cost, real-time detection, and broad prospects for use.

This invention discloses a class of environmentally sensitive MrgX2 fluorescent probes, their preparation methods, and applications, belonging to the field of biomedical and chemical probe technology. Using a small molecule compound with MrgX2 receptor antagonistic activity as a recognition backbone, a long-chain polyethylene glycol linker is introduced by recognizing the active O or N sites on the aromatic ring substituents in the backbone molecule, and then covalently coupled to a Nile Red fluorescent chromophore to obtain the environmentally sensitive MrgX2 fluorescent probe. Compared with existing probes, the fluorescence emission of this probe is located in the red light region, which can reduce the interference of autofluorescence in biological samples and improve the detection signal-to-noise ratio. Simultaneously, the long-chain polyethylene glycol linker improves the probe's hydrophilicity and dispersibility, reducing non-specific membrane staining and background adsorption, which is beneficial for achieving stable and specific fluorescent labeling of the MrgX2 receptor on the cell membrane surface, making it suitable for fluorescent labeling and visualization analysis of the MrgX2 receptor in cell models.

Aspects of the disclosure relate to a method for evaluating two or more target proteins of interest from the same family in a specified functional form, the method comprising: (i) contacting a composition comprising or suspected of comprising the two or more proteins of interest with a molecular construct comprising: a targeting group operatively linked to a retrieval tag; wherein the targeting group specifically binds to the specialized functional form of the two or more target proteins of interest; (ii) contacting the composition with at least two antibody-oligo constructs, wherein at least one of the constructs comprises a first antibody operatively linked to a first oligo and at least a second construct comprises a second antibody operatively linked to a second oligo; wherein the first antibody specifically binds to one of the two or more target proteins of interest and the second antibody specifically binds to the other of the two or more target proteins of interest; (iii) contacting the composition with a second molecular construct comprising a retrieval tag binder operatively linked to a retrieval oligo; (iv) incubating the composition under conditions sufficient for the ligation or annealing of the first oligo to the retrieval oligo when the first and retrieval oligos are in close proximity to each other and ligation or annealing of the second oligo to the retrieval oligo when the second and retrieval oligos are in close proximity to each other; and (v) detecting the ligated or annealed first and retrieval oligo and the ligated or annealed second and retrieval oligo.

Affinity-based and activity-based probes (ABPs) described herein offer transformative resolutions to microbiome function. These ABPs target key metabolic pathways in the gut microbiome. The ABPs contain a binding group, a reactive group, and a. reporter group handle that allows for the addition of a "flexible" reporter group that can be easily swapped to enable multimodal fluorescence and proteomic measurements and isolation of live cells. The binding group, also called an affinity element or biorecognition element, mirrors monomeric and polymeric carbohydrates, sulfated and acetylated carbohydrates, and peptides to afford probe selectivity.