26 results about "Chemical probe" patented technology

Affinity-based and activity-based probes (ABPs) described herein offer transformative resolutions to microbiome function. These ABPs target key metabolic pathways in the gut microbiome. The ABPs contain a binding group, a reactive group, and a. reporter group handle that allows for the addition of a "flexible" reporter group that can be easily swapped to enable multimodal fluorescence and proteomic measurements and isolation of live cells. The binding group, also called an affinity element or biorecognition element, mirrors monomeric and polymeric carbohydrates, sulfated and acetylated carbohydrates, and peptides to afford probe selectivity.

The application relates to the technical field of free radical detection, in particular to a high-sensitivity in-situ capture and detection method of organic free radicals based on a DIPPMPO probe and application, and the method comprises the following steps: adding a chemical probe DIPPMPO into a reaction system containing potential organic free radical precursors, so that the DIPPMPO and organic free radicals generated in a reaction process occur adduct reaction to form a DIPPMPO-free radical adduct which can be detected under liquid chromatography-mass spectrometry conditions; performing high performance liquid chromatography-quadrupole-time of flight mass spectrometry analysis on a mixed solution after the adduct reaction is completed, so that first mass spectrometry data and secondary mass spectrometry fragment information of the DIPPMPO-free radical adduct are obtained; and automatically processing the secondary mass spectrometry data, screening out parent ions with continuous neutral loss as potential DIPPMPO-free radical adduct candidate signals. The application does not need derivatization, is simple to operate, is broad-spectrum and high-efficiency, and can be applied to environmental water samples, biological fluids, chemical reaction liquids or drug metabolism systems.

The application discloses a polymer probe and a preparation method and application thereof, and belongs to the technical field of chemical probes.The structural formula of the polymer probe provided by the application is shown as formula (I): in the formula (I), x and y are each independently selected from any integer in 1-3000.The polymer probe has good water solubility, obvious visual color change for sulfur dioxide and derivatives thereof, and can be used for qualitative or quantitative detection of sulfur dioxide and derivatives thereof in water; meanwhile, the polymer probe has thermal response performance, can be recycled and reused by heating after a reaction of the polymer probe with sulfur dioxide and derivatives thereof in an aqueous solution is completed, is used for secondary analysis and identification of sulfur dioxide and derivatives thereof, has good reusability, is favorable for cost reduction, and has wide application in detection of sulfur dioxide and derivatives thereof.

A tetra-functional compound contains a ligand-binding moiety (A) or ligand, a reactive moiety (D), a cleavable moiety (E), and a biotin tag (B), which are linked optionally via a spacer, wherein the ligand-binding moiety (A) is an activated functional group such as an amine-reactive group, or a reactive functional group such as —COOH; the reactive moiety (D) is a group having the structure of a compound such as 2-aryl-5-carboxytetrazole; the cleavable moiety (E) is a group having the structure of a compound such as 1-(4,4-dimethyl-2,6-dioxocyclohex-1-ylidene)ethyl; and the spacer is a group such as a linear or branched alkylene group having one or more carbon atoms, substituted with a crosslinking group.

Aspects of the disclosure relate to chemical probes for the detection of proteins of interest. The chemical probes can be employed in activity-dependent proximity ligation methods for quantifying proteins of interest in single cells. In some embodiments, the chemical probes can be employed in identifying enzymes in single cells.

The application discloses a crotonylation detection probe and a preparation method and application thereof, and the preparation method comprises the following steps: taking an alkyne alcohol (compound II) as raw material, reacting with methoxycarbonylmethylidene triphenyl positive phosphorus under the action of an oxidant to obtain a methyl ester compound (compound III), hydrolyzing the methyl ester compound (compound III) under the action of alkali to obtain an acid (compound IV), and finally reacting the acid (compound IV) with alkali, filtering and freeze-drying to obtain the probe molecule (compound I). The chemical probe (compound I) with an alkyne group is applied in cells, the alkyne group is selectively loaded on target proteins at specific modification sites through metabolic labeling, and through a bio-orthogonal chemical reaction, a fluorescent dye can be orthogonally connected to the modification site, so that the probe is used for fluorescent imaging of crotonylation proteins in cells and identification of crotonylation modification substrates and sites, and by using the probe, crotonylation sites regulated by histone deacetylases (HDACs) can be determined through quantitative chemical proteomics.

This invention discloses a Pt-Ni@erGO electrochemical probe, its preparation method, and its applications. The Pt-Ni@erGO electrochemical probe comprises an erGO matrix and Pt-Ni nanoparticles supported on the erGO matrix. The Pt-Ni@erGO electrochemical probe of this invention uses erGO as a matrix to grow Pt-Ni alloy nanoparticles in situ. erGO simultaneously exhibits two functions to enhance electrochemical detection sensitivity: firstly, it acts as a carrier to load high-density Pt-Ni alloy nanoparticles, reducing the shielding effect of biorecognition elements on the catalytic sites of nanozymes; secondly, it acts as a biorecognition element to specifically bind to *E. coli*. Through the rational combination of erGO and Pt-Ni nanoparticles, the obtained Pt-Ni@erGO possesses high antibody-like activity and electrocatalytic activity.

The application discloses a surface fluorescence chromatography resin embedding method suitable for immunostaining samples, and comprises the following steps: S1, biological tissue sample pretreatment; S2, gradient ethanol dehydration and tissue blackening; S3, gradient resin permeation; S4, acidification treatment; and S5, resin polymerization molding. The application also comprises a biological tissue sample imaging method, which comprises the step of imaging the biological tissue sample obtained by the embedding method in a fMOST system sodium carbonate solution environment. The application temporarily quenches the pH-sensitive chemical probe by adding HAc; in combination with the fMOST imaging system, the reactivation is realized in the alkaline solution environment, the blackening chemical chromatography high-precision imaging is realized by reactivating the sample imaging surface, and the immunostaining sample has the advantages of high fluorescence retention rate.

The invention discloses a PROTAC compound for degrading TRIM15 protein. The PROTAC compound has a structure as shown in a formula (I). The molecule can effectively inhibit the RIG-1 signal channel, and has application prospects in preparation of anti-inflammatory or autoimmune disease drugs. Meanwhile, the invention further provides a chemical probe molecule with the structure shown in the formula (III). One end of the molecule is combined with TRIM15, and the other end of the molecule can be combined with tag protein (such as Halog-tag) or other target protein, so that TRIM15 is recruited as E3 ubiquitin ligase to carry out ubiquitination modification on the tag protein (or the target protein). The molecule can be used as an innovative research tool and is used for exploring the biological function of the E3 ligase of TRIM15, the substrate specificity and non-K48 type ubiquitination modification.

The present application belongs to the technical field of chemical probes, and particularly relates to a preparation method of a probe for analyzing intestinal flora regulating BSH activity. The preparation method of the probe for analyzing intestinal flora regulating BSH activity provided by the present application uses 7-amino-4-methyl-3-coumarin acetic acid (AMCA) as raw material, sequentially carries out a first reaction with a chlorinating agent and an alcohol reagent, and carries out a second reaction with a CA compound in the presence of a condensing agent and a catalyst, and the required CA-AMCA probe can be obtained through a hydrolysis reaction. The preparation method of the BSH probe provided by the present application not only has easily obtained raw materials and a simple process route, but also has a total reaction yield as high as 64%, which can effectively guarantee the product yield.

The application relates to a silver-doped hollow carbon nanosphere electrochemical probe and a preparation method thereof, and belongs to the technical field of electroanalysis chemical detection and carbon nanomaterials. The Ag@HCS composite material is prepared by using a SiO2 template method, polymerizing formaldehyde and m-diphenol to form a phenolic resin ball in the presence of silver nitrate, and then performing carbonization treatment and in-situ reduction of silver and removing the template. The Ag@HCS is used for HbA 1c The determination of HbA 1c is realized based on the silver-based electrochemical oxidation signal of the electrochemical probe after the modification of the nucleic acid aptamer. Experiments show that the electrochemical sensor constructed by the method has high sensitivity, a wide detection range, high specificity and good stability, and exhibits good feasibility in the detection of actual samples, and the method provides a method for synthesizing a stable, controllable and sensitive electrochemical probe.

The invention belongs to the technical field of protein detection, and particularly relates to a chemical probe, a preparation method of the chemical probe and application of the chemical probe in detection of Tau protein aggregates. The chemical probe can specifically recognize the Tau protein aggregate, and after the chemical probe is combined with the Tau protein aggregate, a fluorescence signal can be remarkably enhanced, so that the Tau protein aggregate is detected; and after long-term illumination, good characteristics can still be maintained, and the stability is good.

This disclosure relates to the field of anticancer drug technology, and discloses a 7-azaindole compound, its synthesis method, and its uses. The structure of the 7-azaindole compound is as follows: This 7-azaindole compound can be used as a fluorescent polarization probe or a degradation chemical probe targeting CSN5; wherein, the fluorescent polarization chemical probe has a good binding affinity to the metalloproteinase CSN5, laying an important foundation for the discovery of novel CSN5 inhibitors; at the same time, the degradation chemical probe exhibits good enzyme-level activity and cell-inhibitory activity, and has the potential to degrade CSN5 protein and its variants, providing a material basis for the development of related drugs targeting CSN5 and the study of its biological functions, and also has great potential in the discovery of cancer immunotherapy drugs and the treatment of other related diseases.

The present disclosure provides a detection method of wormlike micelles, and belongs to the technical field of analytical chemistry. In the present disclosure, z-ArN2+ is used as a chemical probe substance; a long-chain probe is added to a test solution for probing an interfacial region, and a short-chain probe is added to a surfactant-free control solution. A selectivity of the probe with different nucleophiles is determined by the above method, and a yield of an obtained product is measured by high-performance liquid chromatography (HPLC); and the wormlike micelles are detected by measuring the yield of the product using the selectivity of the short-chain probe to be converted into an interfacial mole number. The wormlike micelles are detected by chemical probes, and the chemical probes have a simple preparation method, a low cost, real-time detection, and broad prospects for use.

This invention discloses a class of environmentally sensitive MrgX2 fluorescent probes, their preparation methods, and applications, belonging to the field of biomedical and chemical probe technology. Using a small molecule compound with MrgX2 receptor antagonistic activity as a recognition backbone, a long-chain polyethylene glycol linker is introduced by recognizing the active O or N sites on the aromatic ring substituents in the backbone molecule, and then covalently coupled to a Nile Red fluorescent chromophore to obtain the environmentally sensitive MrgX2 fluorescent probe. Compared with existing probes, the fluorescence emission of this probe is located in the red light region, which can reduce the interference of autofluorescence in biological samples and improve the detection signal-to-noise ratio. Simultaneously, the long-chain polyethylene glycol linker improves the probe's hydrophilicity and dispersibility, reducing non-specific membrane staining and background adsorption, which is beneficial for achieving stable and specific fluorescent labeling of the MrgX2 receptor on the cell membrane surface, making it suitable for fluorescent labeling and visualization analysis of the MrgX2 receptor in cell models.

Aspects of the disclosure relate to a method for evaluating two or more target proteins of interest from the same family in a specified functional form, the method comprising: (i) contacting a composition comprising or suspected of comprising the two or more proteins of interest with a molecular construct comprising: a targeting group operatively linked to a retrieval tag; wherein the targeting group specifically binds to the specialized functional form of the two or more target proteins of interest; (ii) contacting the composition with at least two antibody-oligo constructs, wherein at least one of the constructs comprises a first antibody operatively linked to a first oligo and at least a second construct comprises a second antibody operatively linked to a second oligo; wherein the first antibody specifically binds to one of the two or more target proteins of interest and the second antibody specifically binds to the other of the two or more target proteins of interest; (iii) contacting the composition with a second molecular construct comprising a retrieval tag binder operatively linked to a retrieval oligo; (iv) incubating the composition under conditions sufficient for the ligation or annealing of the first oligo to the retrieval oligo when the first and retrieval oligos are in close proximity to each other and ligation or annealing of the second oligo to the retrieval oligo when the second and retrieval oligos are in close proximity to each other; and (v) detecting the ligated or annealed first and retrieval oligo and the ligated or annealed second and retrieval oligo.

Affinity-based and activity-based probes (ABPs) described herein offer transformative resolutions to microbiome function. These ABPs target key metabolic pathways in the gut microbiome. The ABPs contain a binding group, a reactive group, and a. reporter group handle that allows for the addition of a "flexible" reporter group that can be easily swapped to enable multimodal fluorescence and proteomic measurements and isolation of live cells. The binding group, also called an affinity element or biorecognition element, mirrors monomeric and polymeric carbohydrates, sulfated and acetylated carbohydrates, and peptides to afford probe selectivity.

The application belongs to the technical field of biological detection, and particularly relates to a probe compound for CoA detection, a fluorescent probe and application, comprising a modified rhodamine series fluorescent dye, a pantothenate kinase inhibitor, a HaloTag, a Nanoluc luciferase and a pantothenate kinase fusion protein; through protein labeling, a series of biosensors are obtained, coenzyme A concentration in living cells can be rapidly detected, and rapid screening of coenzyme A drugs is regulated; through rational design and modification of the chemical probe molecules, and engineering modification of the sensor proteins, the chemical probes can label the sensor proteins in living cells, and then the bioluminescent sensors working in living cells are obtained, the requirements of rapid and high-sensitivity detection of CoA can be met, and the drugs capable of regulating the change of coenzyme A concentration can be high-throughput screened.

The present invention relates to compounds of formula (I) salts and derivatives thereof. Further object of the present invention are compositions, which comprise at least a compound of formula (I) or its pharmaceutically acceptable salts and derivatives, and at least one excipient. Further object of the present invention are compounds of formula (I) or compositions which comprise them, for use in the treatment or prevention of a cancer and / or a metabolic disease and / or an immunological disease. Further object of the present invention is the in vitro use of the compounds as chemical probes.

This invention provides an integrated molding method for a sterile medical blister pack, relating to the field of blister molding technology, comprising the following steps: S1: Calculating the chemical migration potential index of the raw materials before the coating material is joined to the pre-formed plastic box body; S2: Calculating the process activation risk index during the heat sealing connection between the coating and the box body; S3: Obtaining the signal intensity of an online chemical probe, and coupling it with the raw material chemical migration potential index and the process activation risk index to calculate the batch migration release index; S4: Obtaining the batch migration release index, clinical adverse event correlation score, increase in migratings from retained sentinel samples, and supply chain reverse verification confirmation rate of the batch, generating a market feedback closed-loop correction coefficient. This invention can assess risks in real time, automatically adjust processes, classify products according to test results, and continuously optimize production standards using feedback from clinical use, thereby achieving intelligent closed-loop control from source to finished product.

An improved chemical probe for the detection of the amino acid citrulline combines: 1) a reactive head formed of 1,3-dicarbonyl moiety that reacts with a citrulline side chain in an improved manner compared to currently used 1,2-dicarbonyl moieties; and 2) a modular action of the probe where citrulline side chains are labeled first using reactive heads described above, and attachment of a read-out subunit or tag, be it a fluorophore, a nanoparticle, or an antigen is performed separately. The modular nature of the chemical probe increases the sensitivity of the probes due to their smaller size. Additionally, the chemical probes of the present disclosure allow the same sample to be analyzed using a variety of read-out methods.

Beta-lactam compounds to detect carbapenemases or microbial carbapenem resistance are disclosed. The compounds contain a chemical probe. Upon hydrolysis by carbapenemases, the compounds undergo intramolecular rearrangement and release the chemical probe. Detection of the released chemical probe indicates the presence of carbapenemases and the presence of microbial carbapenem resistance.

The invention relates to the technical field of biology, in particular to a polypeptide skeleton chemical probe, a preparation method and application of the polypeptide skeleton chemical probe in receptor membrane protein identification. The polypeptide skeleton chemical probe comprises an enrichment module, a ligand coupling module, a polypeptide skeleton and a receptor crosslinking module. The polypeptide skeleton chemical probe is multifunctional, an enrichment module, a ligand coupling module and a receptor cross-linking module are connected to a polypeptide skeleton, and the enrichment module serves as a label and can be used for purification cross-linking of streptavidin beads and the like; the ligand coupling module can be used for carrying out chemical coupling with target secretory protein according to needs, the receptor crosslinking module enables the polypeptide skeleton chemical probe to be combined with a receptor and to be subjected to photo-crosslinking, enzymatic crosslinking and the like, and the polypeptide skeleton is a main skeleton and a connecting shaft of the whole probe and connects all functions together.

The invention relates to methods to determine KDM1A target engagement and chemoprobes useful therefor. In particular, the invention relates to non-peptidic KDM1A chemoprobes carrying a tag or label that can be used to assess KDM1A target engagement in cells and tissues. These chemoprobes can also be used to identify KDM1A interacting factors and analyze expression levels of KDM1A.

The application provides an X-ray click chemistry probe based on a synchronous light source and a preparation method and application thereof, and comprises the following steps: 1) synthesizing a polydopamine-metal with a group required for a click reaction on a polydopamine surface; 2) adding a modification reagent to a culture medium with cells to modify biomolecules which can be targeted to various subcellular organelles; 3) adding the polydopamine-metal synthesized in step 1) to the culture medium after modification in step 2) until a click reaction is completed; and 4) adding the cells obtained in step 3) to a substrate suitable for synchronous radiation X-ray imaging, and observing imaging under synchronous radiation X-ray, so that biomolecules in the cells have specific absorption peaks under X-ray, and the method is obtained. According to the application, a method for high-specificity recognition and high-resolution imaging of various biomolecules in cells based on click chemistry and synchronous X-ray is provided, and has a good biomedical application prospect.