Disulfide dot/nanosheet composite dna electrochemical probe and its preparation method and application

A disulfide and nanosheet technology, applied in the field of analytical chemistry, can solve the problems that transition metal disulfide quantum dots/nanosheet composite bioelectrochemical probes have not been seen, and achieve high sensitivity, broad application prospects, Wide linear range effect
CN105004775BActive Publication Date: 2016-02-03QINGDAO UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
QINGDAO UNIV
Publication Date
2016-02-03

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Abstract

The invention relates to a preparation method of a disulfide dot / nano sheet composite DNA electrochemical probe. The method specifically includes the following steps: 1) ultrasonically stripping the transition metal disulfide powder, high-temperature electromagnetic stirring to prepare a transition metal disulfide quantum dot / nanosheet composite, ultrasonically in a dispersion of thionine and an ionic liquid, Prepare a thionine functionalized complex; 2) Disperse the prepared complex in an organic solvent, make a suspension and drop-coat it on the electrode, and obtain a complex modified electrode after drying; 3) Prepare the above prepared electrode In the three-electrode system, the electrochemical test is performed in the buffer solution, and DNA solutions of different concentrations are injected for testing, and the electrochemical signal changes on the electrode surface are recorded to construct a DNA electrochemical probe system. The probe of the invention has the advantages of high sensitivity, good stability, wide linear range, low detection limit, etc., can be used for detecting DNA in serum, and has broad application prospects in the fields of biological analysis and clinical diagnosis.
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Description

technical field

[0001] The invention belongs to the technical field of analytical chemistry, and in particular relates to a disulfide dot / nano sheet composite DNA electrochemical probe and a preparation method and application thereof. Background technique

[0002] Deoxyribose nucleic acid (Deoxyribonucleic acid, abbreviated as DNA) is the basic genetic material of all organisms. DNA detection plays a vital role in the fields of medical diagnosis, bioengineering and environmental protection. As a new type of detection method, DNA electrochemical bioprobe has many advantages such as simple equipment required, fast and sensitive detection, and related technologies are developing very rapidly. In recent years, the development of nanoscience and technology is changing with each passing day, which provides a new and effective way for the development of new biological probes with high sensitivity, high stability, low cost and good safety. At present, DNA bioprobes based on nanomat...

Claims

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