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Disulfide dot/nanosheet composite dna electrochemical probe and its preparation method and application

A disulfide and nanosheet technology, applied in the field of analytical chemistry, can solve the problems that transition metal disulfide quantum dots/nanosheet composite bioelectrochemical probes have not been seen, and achieve high sensitivity, broad application prospects, Wide linear range effect

Active Publication Date: 2016-02-03
QINGDAO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Up to now, transition metal disulfide quantum dots (especially titanium disulfide and tantalum disulfide) have been less studied in the field of biosensing. Bioelectrochemical probes have not yet been reported in domestic and foreign literature and related patents

Method used

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  • Disulfide dot/nanosheet composite dna electrochemical probe and its preparation method and application
  • Disulfide dot/nanosheet composite dna electrochemical probe and its preparation method and application
  • Disulfide dot/nanosheet composite dna electrochemical probe and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031]Weigh 200mg of molybdenum disulfide powder and disperse it in 10mL of organic solvent N-methylpyrrolidone. After ultrasonic stripping for 3 hours, let the dispersion stand for 10 minutes, collect most of the dispersion in the upper layer, and remove the large particles that are not completely peeled off at the bottom to obtain a single layer or several layers of molybdenum disulfide nanosheet dispersion. Stir the dispersion liquid vigorously at 135°C for 7 hours to obtain a composite solution of molybdenum disulfide quantum dots / nanosheets, add acetone to the above composite solution, and the composite aggregates to the top layer of the solution, separate and dry at room temperature to obtain Composite of molybdenum disulfide quantum dots / nanosheets. Take 100mg of the molybdenum disulfide quantum dot / nanosheet composite prepared above and dissolve it in 10mg·mL -1 In the mixed solution of thionine acetate and ionic liquid 1-butyl-3-methylimidazolium hexafluorophosphate,...

Embodiment 2

[0036] Weigh 200mg of titanium disulfide powder and disperse it in 10mL organic solvent N,N-dimethylformamide. After ultrasonic stripping for 6 hours, let the dispersion stand for 10 minutes, collect most of the dispersion in the upper layer, and remove the large particles that are not completely peeled off at the bottom. , to obtain a single-layer or several-layer titanium disulfide nanosheet dispersion. Vigorously stir the dispersion at 125°C for 8 hours to obtain a composite solution of titanium disulfide quantum dots / nanosheets, add chloroform to the above composite solution, the composite gathers to the top layer of the solution, separate, and dry at room temperature to obtain Composite of titanium disulfide quantum dots / nanosheets. Take 200mg of the titanium disulfide quantum dot / nanosheet composite prepared above and dissolve it in 10mg·mL -1 In the mixed solution of thionine acetate and ionic liquid 1-butyl-3-methylimidazolium hexafluorophosphate, the above mixture is...

Embodiment 3

[0040] Weigh 200mg of tantalum disulfide powder and disperse it in 10mL organic solvent N,N-dimethylformamide. After ultrasonic stripping for 9 hours, let the dispersion stand for 10 minutes, collect most of the dispersion in the upper layer, and remove the large particles that are not completely peeled off at the bottom. , to obtain a single-layer or several-layer molybdenum disulfide nanosheet dispersion. Vigorously stir the dispersion at 140°C for 6 hours to obtain a composite solution of tantalum disulfide quantum dots / nanosheets, add acetone to the above composite solution, the composite gathers to the top layer of the solution, separate, and dry at room temperature to obtain Composites of tantalum disulfide quantum dots / nanosheets. Take 100 mg of the tantalum disulfide quantum dot / nanosheet composite prepared above and dissolve it in 10 mg·mL -1 In the mixed solution of thionine acetate and ionic liquid 1-butyl-3-methylimidazolium hexafluorophosphate, the above mixture ...

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Abstract

The invention relates to a preparation method of a disulfide dot / nano sheet composite DNA electrochemical probe. The method specifically includes the following steps: 1) ultrasonically stripping the transition metal disulfide powder, high-temperature electromagnetic stirring to prepare a transition metal disulfide quantum dot / nanosheet composite, ultrasonically in a dispersion of thionine and an ionic liquid, Prepare a thionine functionalized complex; 2) Disperse the prepared complex in an organic solvent, make a suspension and drop-coat it on the electrode, and obtain a complex modified electrode after drying; 3) Prepare the above prepared electrode In the three-electrode system, the electrochemical test is performed in the buffer solution, and DNA solutions of different concentrations are injected for testing, and the electrochemical signal changes on the electrode surface are recorded to construct a DNA electrochemical probe system. The probe of the invention has the advantages of high sensitivity, good stability, wide linear range, low detection limit, etc., can be used for detecting DNA in serum, and has broad application prospects in the fields of biological analysis and clinical diagnosis.

Description

technical field [0001] The invention belongs to the technical field of analytical chemistry, and in particular relates to a disulfide dot / nano sheet composite DNA electrochemical probe and a preparation method and application thereof. Background technique [0002] Deoxyribose nucleic acid (Deoxyribonucleic acid, abbreviated as DNA) is the basic genetic material of all organisms. DNA detection plays a vital role in the fields of medical diagnosis, bioengineering and environmental protection. As a new type of detection method, DNA electrochemical bioprobe has many advantages such as simple equipment required, fast and sensitive detection, and related technologies are developing very rapidly. In recent years, the development of nanoscience and technology is changing with each passing day, which provides a new and effective way for the development of new biological probes with high sensitivity, high stability, low cost and good safety. At present, DNA bioprobes based on nanomat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/327
Inventor 王宗花赵春芹桂日军金辉杨敏夏延致
Owner QINGDAO UNIV
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