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Thiamphenicol molecularly imprinted electrochemical sensor and preparation method and application thereof

A thiamphenicol, molecular imprinting technology, applied in the direction of material electrochemical variables, scientific instruments, instruments, etc., can solve problems such as human health effects, and achieve the effect of broadening detection channels, improving sensitivity, and improving selectivity

Active Publication Date: 2017-03-22
HONGHE COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, excessive intake can have a great impact on human health

Method used

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  • Thiamphenicol molecularly imprinted electrochemical sensor and preparation method and application thereof
  • Thiamphenicol molecularly imprinted electrochemical sensor and preparation method and application thereof
  • Thiamphenicol molecularly imprinted electrochemical sensor and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] (1) Preparation of porous Pt-Pd NPs: 5.0 mL hexadecylpyridine (HDPC) (4.0 mg mL -1 ), 0.40mLNa 2 PdCl 4 (10mmol L -1 ) and 0.40mL H 2 PtCl 6 (10mmol L -1 ) into a round bottom flask to form a uniform dispersion; then quickly add 0.60mL of freshly prepared AA solution to the above solution, shake gently to make it evenly dispersed, and place the round bottom flask Reaction in an oil bath at 85°C for 3 h; then, the obtained sol was centrifuged and washed with water several times to obtain dendritic Pt-Pd NPs;

[0043] (2) Preparation of porous graphene oxide: under magnetic stirring, 0.5g KMnO 4 Add 100mL 0.5mg mL - 1 GO dispersion and allowed to react for 12 h. Then, 30mL HCl (36%, wt%) and 30mL H 2 o 2 (30%, wt%) was added to the above reaction solution, and the reaction was continued for 3h. After the reaction, the obtained product was centrifuged, washed with water, and dried in a vacuum dryer at 60°C; a porous graphene oxide dispersion was prepared;

[0...

Embodiment 2

[0048] (1) Preparation of porous Pt-Pd NPs: Hexadecylpyridine (HDPC, 10molL -1 ), Na 2 PdCl 4 (10molL -1 ) and H 2 PtCl 6 (10molL -1 ) into a round bottom flask according to the volume ratio of 3:1:1 to form a uniform dispersion; then the freshly prepared ascorbic acid (AA) solution is quickly added to the above solution, the volume ratio of AA solution to HDPC is 1:25, shake gently to make it evenly dispersed, and place the round-bottomed flask in an 80°C oil bath for 2.5h; then, centrifuge the obtained sol and wash it with water several times to obtain dendritic Pt-Pd NPs;

[0049] (2) Preparation of porous graphene oxide:

[0050] Under magnetic stirring, the KMnO 4 Added to the GO dispersion and allowed to react for 6h, wherein, KMnO 4 The mass ratio to GO dispersion is 8:1; then, HCl (36%, wt%) and H 2 o 2 (30%, wt%) was added in the above-mentioned reaction solution, continued reaction 3h, wherein GO dispersion liquid and HCl and H 2 o 2 The volume ratio of ...

Embodiment 3

[0055] (1) Preparation of porous Pt-Pd NPs: Hexadecylpyridine (HDPC, 10molL -1 ), Na 2 PdCl 4 (10molL -1 ) and H 2 PtCl 6 (10molL -1 ) into a round bottom flask according to the volume ratio of 8:1:1 to form a uniform dispersion; then the freshly prepared ascorbic acid (AA) solution is quickly added to the above solution, the volume ratio of AA solution to HDPC is 1:5, shake gently to make it evenly dispersed, and place the round bottom flask in a 90°C oil bath for 3.5h; then, centrifuge the obtained sol and wash it with water several times to obtain dendritic Pt-Pd NPs;

[0056] (2) Preparation of porous graphene oxide:

[0057] Under magnetic stirring, the KMnO 4 Added to the GO dispersion and allowed to react for 12h, wherein, KMnO 4 The mass ratio to GO dispersion is 15:1; then, HCl (36%, wt%) and H 2 o 2 (30%, wt%) was added in the above-mentioned reaction solution, continued reaction 3h, wherein GO dispersion liquid and HCl and H 2 o 2 The volume ratio of ea...

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Abstract

The invention discloses a thiamphenicol molecularly imprinted electrochemical sensor and a preparation method and application thereof. A porous graphene-molybdenum disulfide nano flower-shaped compound is prepared through a hydrothermal one-step method, and the compound is dropped on the surface of an L-type glassy carbon electrode; aminated multi-walled carbon nanotubes and porous Pt-Pd nanoparticles are dispersed so that the imprinting surface area of the electrode can be increased and stability of a modification interface can be enhanced, and accordingly a three-dimensional porous imprinting substrate is obtained; thiamphenicol serves as template molecules, 1,2-diaminobenzene serves as functional monomers, and an imprinted membrane is prepared through cyclic voltammetry; ascorbic acid serves as a photoelectric chemical probe, and the electrochemical sensor for detecting thiamphenicol is built. The sensor has good thiamphenicol responsiveness, the linear range of the sensor is 1.0*10<-9>-3.5*<-6> mol L<-1>, and the detection lower limit is 5.0*10<-9> mol L<-1>. The sensor can be used for detecting thiamphenicol in meat samples and feed samples.

Description

technical field [0001] The invention belongs to the technical field of molecular imprinting, and in particular relates to a thiamphenicol molecular imprinting electrochemical sensor and its preparation method and application. Background technique [0002] Thiamphenicol is a commonly used antibiotic, which belongs to broad-spectrum antibiotics with chloramphenicol and florfenicol, and has good antibacterial effect, so it is widely used in the treatment of human diseases and animal food production, and forms residues. At the same time, under the background that chloramphenicol was banned in the production of animal-derived food by China, Canada, the United States and the European Union, it gradually became a substitute for chloramphenicol in the production of animal-derived food. However, excessive intake can have a great impact on human health. Therefore, countries such as the United States, Canada, and China have banned its use in the production of animal-derived foods and ...

Claims

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Application Information

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IPC IPC(8): G01N27/30
CPCG01N27/308
Inventor 杨光明刘卫徐世娟陈显兰石玲
Owner HONGHE COLLEGE
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