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The application discloses an N-Boc-L-phenylalanine molecular imprinting polymer and a preparation method and application thereof. The N-Boc-L-phenylalanine molecular imprinting polymer is prepared by using a bulk polymerization method, taking N-Boc-L-phenylalanine as a template molecule and allylamine as a functional monomer, and under the participation of a crosslinking agent and an initiator. The molecular imprinting polymer has specific recognition ability to N-Boc-L-phenylalanine. The maximum adsorption capacity of the molecular imprinting polymer to N-Boc-L-phenylalanine reaches 14.50 mg / g, and the imprinting factor IF value is as high as 10.33. The adsorption amount of the molecular imprinting polymer to N-Boc-L-phenylalanine is significantly higher than that to N-Boc-D-phenylalanine, and the molecular imprinting polymer has good chiral recognition ability. The molecular imprinting polymer can be used for selective recognition, adsorption and enrichment of N-Boc-L-phenylalanine.