189 results about "Microbiological contamination" patented technology

The invention provides a puree production line microbial pollution risk real-time monitoring and early warning method, which comprises the following steps: deploying multiple types of sensors, an industrial camera and an operation log interface, collecting environmental temperature and humidity, pH value, dissolved oxygen, image and operation behavior multi-modal data in a fermentation tank in real time, and carrying out edge calculation and standardized preprocessing to obtain the microbial pollution risk of a puree production line. Data missing, abnormity and time sequence difference are eliminated; multi-modal features and a dynamic knowledge graph constructed based on a production process and a pollution event are fused, and a deep learning model and a graph neural network are utilized to realize microbial pollution risk probability intelligent prediction; risk grading and a dynamic weighting algorithm are introduced, grading early warning signals and disposal suggestions are automatically generated, and a production control system is linked to execute response measures; according to the subsequent production state, continuous feedback is carried out, the model and the early warning threshold are adjusted in a self-adaptive mode, and the accuracy, response efficiency and production safety of pollution early warning are remarkably improved.

The invention discloses a food safety risk assessment method and system based on model analysis, and relates to the technical field of food safety production monitoring, a production line virtual model synchronized with a physical production line in real time is constructed based on the structural parameters of a target production line, and the deviation of production line data is corrected through a model fusion algorithm, so that the risk assessment accuracy is improved. And identifying abnormal physical parameter inflection points strongly related to microbial pollution. According to the method, a production line virtual model is constructed through digital twinning, a model fusion algorithm is introduced, time-domain and frequency-domain multi-dimensional comparison and correction are performed on real-time misalignment data and historical steady-state data, instantaneous interference and systematic drifting are effectively filtered out, the corrected production line data are output, and the real-time misalignment data and the historical steady-state data are compared and corrected. And on the basis, abnormal physical parameter inflection points strongly related to microbial pollution are accurately identified, so that weak abnormal signals which are difficult to perceive by a traditional method can be captured in the initial stage of continuous production, and the early pollution risk perception capability for pathogenic bacteria is remarkably improved.

The present invention provides improved and / or shortened methods for expanding TILs and producing therapeutic populations of TILs, including novel methods for expanding TIL populations in a closed system that lead to improved efficacy, improved phenotype, and increased metabolic health of the TILs in a shorter time period, while allowing for reduced microbial contamination as well as decreased costs. Such TILs find use in therapeutic treatment regimens.

Compositions, testing chambers and methods for testing a fuel sample for microbial contamination (including fuels treated with a biocide) are provided, which comprise: a quantity of hydrocarbon fuel; a microbial contamination wherein the microbial contamination further comprises nucleic acid in the form of both DNA, RNA or a combination thereof, and an analyzing solution; wherein the analyzing solution comprises at least six (6) primer pairs for amplification of at least one target locus, wherein at least one primer of each pair of primers is labeled with a fluorescent dye and wherein at least one of the primer pair binds to the nucleic acid of the microbial contamination.

The application provides a rapid microbial contamination detection method and system based on ATP fluorescence detection. The optimal reaction conditions of different types of microbial samples are determined, a to-be-detected sample and an ATP fluorescence detection reagent are mixed and reacted to generate a reaction mixture with maximum ATP release efficiency; the gain level is automatically adjusted according to the preliminarily measured fluorescence signal intensity, the fluorescence signal intensity value of the reaction mixture is measured to generate an optimized fluorescence signal intensity value; the preliminary prediction result of the microbial contamination degree is generated by comparing and analyzing the optimized fluorescence signal intensity value with a preset microbial ATP content standard curve; the credibility of the detection result is evaluated, and the detection result is sent to a remote monitoring platform to realize real-time monitoring and early warning of the microbial contamination situation. The technical scheme provided by the application improves the accuracy and response speed of microbial contamination detection.

The invention relates to the technical field of food preservation, in particular to a double-layer fiber membrane for food preservation and a preparation method and application of the double-layer fiber membrane. The first layer of the double-layer fiber membrane for food preservation is composed of sericin / pullulan, and the second layer is a polylactic acid-based membrane doped with FA-coated Ag-ZIF-8 nanoparticles. The double-layer fiber membrane realizes high selectivity to oxygen and carbon dioxide, realizes regulation and control of microenvironment gas atmosphere, and inhibits respiration, so that an excellent fresh-keeping effect is achieved. The double-layer fiber membrane has antibacterial and antioxidant functions, and can inhibit oxidative degradation and microbial contamination of perishable fruits. According to the technology, dual mechanisms of gas regulation and control and biological protection are integrated, an innovative scheme is provided for solving the rotting problem of perishable fruits, and the technology shows important potential in the aspects of promoting development of a food fresh-keeping technology and optimizing a sustainable packaging scheme.

The invention discloses a computer vision-based drug surface microbial contamination detection method, which comprises the following steps of: S1, acquiring a multi-angle drug surface image, and preprocessing to generate a standardized multi-modal image sample set; s2, constructing a phase feature modeling network based on improved PV-Net, and extracting a phase response diagram and a structure texture diagram; s3, constructing a candidate pollution region mask based on the phase response diagram and the structure texture diagram, and executing multi-modal region extraction; s4, executing channel alignment and boundary completion operations, and fusing to generate a cross fusion tensor; s5, inputting the cross fusion tensor into a pollution characteristic discrimination network, and outputting a pollution mask, a type label and a pollution level score; and S6, image rendering and pollution area visual output are completed according to a detection result, and structured detection data recording is supported. According to the invention, accurate identification and efficient detection of drug micro-pollution are realized, and the detection accuracy and the online application efficiency are improved.

Compositions, testing chambers and methods for testing a fuel sample for microbial contamination (including fuels treated with a biocide) are provided, which comprise: a quantity of hydrocarbon fuel; a microbial contamination wherein the microbial contamination further comprises nucleic acid in the form of both DNA, RNA or a combination thereof, and an analyzing solution; wherein the analyzing solution comprises at least six (6) primer pairs for amplification of at least one target locus, wherein at least one primer of each pair of primers is labeled with a fluorescent dye and wherein at least one of the primer pair binds to the nucleic acid of the microbial contamination.

This invention relates to a method for detecting colorectal cancer tumor cell-associated microorganisms, specifically a method for eliminating intestinal fecal microbial contamination and detecting colorectal cancer tumor-associated microorganisms, belonging to the field of biotechnology. This invention initially eliminates fecal microbial contamination from the sample structure by coarsely shearing and enzymatically digesting the intestinal folds and villi; then, it uses a 40μm cell sieve to filter out incompletely digested tissue fragments, further eliminating intestinal fecal bacteria contamination, and collecting a single-cell suspension; subsequently, the collected single-cell suspension is repeatedly washed with 300g centrifugation to remove as many intercellular or loosely bound "transient bacteria" as possible, retaining intracellular and firmly colonized microorganisms. Ultimately, this eliminates the severe contamination from the large and complex fecal microorganisms, allowing the detection of tumor cell-associated microorganisms, which are already present in low biomass. This method is suitable for detecting microorganisms in gastrointestinal tissues that are in direct contact with the environment.

The invention relates to the technical field of seedling raising, and discloses an efficient tissue culture and rapid propagation seedling raising method for xanthoceras sorbifolia bunge, which comprises the following steps: selecting and pretreating explants, selecting current-year robust branches of the xanthoceras sorbifolia bunge, cutting stem segments with axillary buds as the explants, cleaning, trimming and disinfecting the explants, performing surface disinfection on the pretreated explants, and performing tissue culture and rapid propagation seedling raising on the pretreated explants to obtain the xanthoceras sorbifolia bunge. When the Xanthoceras sorbifolia Bunge is subjected to efficient tissue culture and rapid propagation seedling culture, an operation standard considering thorough sterilization and tissue activity protection is established by standardizing an explant selection standard and executing a step-by-step surface disinfection procedure, so that the microbial contamination rate caused by incomplete disinfection of the explant can be reduced, meanwhile, tissue activity damage caused by excessive disinfection is reduced, and the survival rate of the Xanthoceras sorbifolia Bunge is improved. Therefore, sufficient and healthy sterile initial materials are guaranteed to be obtained, the problems of high primary culture pollution rate and induction starting failure are solved, and a reliable foundation is laid for establishment of a whole tissue culture and rapid propagation system.

The invention relates to the technical field of baijiu production, in particular to a process for brewing tricholoma matsutake baijiu with distilled water, which comprises the following steps: firstly, selecting raw materials for pretreatment, using distilled water in the whole process, fermenting according to the flavor type requirement, distilling to obtain a stock solution, blending and ageing, and finally filling and packaging to obtain a finished product, and each link ensures the quality and flavor. By controlling the chloride ion concentration and the ferrous ion concentration in the solution, chlorides in the molten salt slag are completely contained in waste acid, insoluble substances are almost non-toxic and can be directly stacked, the distilled water white spirit brewing process can improve the purity of the white spirit, reduce impurities and peculiar smell, ensure stable quality, show fine and mellow taste and meet the hygienic standard, and the distilled water white spirit brewing process is suitable for large-scale popularization and application. The microbial pollution risk is reduced, the health is guaranteed, the requirements of different aroma types can be met, respective styles and characteristics are highlighted, the brand image can be enhanced, the storage life of the white spirit is prolonged, consumers are more relieved and satisfied, and products with higher quality are brought to the white spirit industry.

The present invention relates to a method for sanitising, disinfecting or sterilising microbially contaminated water-containing fluids, comprising treating the microbially contaminated water-containing fluid with an oxidising agent, wherein a fluorescence measurement is carried out on the water-containing fluid before, after and / or during treatment of the microbially contaminated water-containing fluid with the oxidising agent. The fluorescence measurement comprises irradiation with an excitation wavelength characteristic of a fluorescent protein and / or peptide building block and measurement of the corresponding fluorescence signal, and the method is characterised in that treatment with the oxidising agent proceeds in such a way that addition of the oxidising agent is controlled as a function of the fluorescence signal. The invention further relates to a corresponding device for sanitising, disinfecting or sterilising microbially contaminated water-containing fluid.

A water recycle system may be implemented to analyze and selectively recycle wastewater generated by an industrial textile washer. In some examples, wastewater generated by the industrial textile washer undergoes various treatment processes to generate a clarified wastewater that is stored before being recycled for reuse in the textile washer. Microbial contamination can grow in this stored water over time and the water can become odorous, which is undesirable when subsequently reusing the water to wash textiles. Systems and techniques can be implemented to treat the clarified water to control odor and / or microbial contamination, improving the effectiveness and quality of the water recycle system.

The invention discloses an edible fish collagen-based antibacterial active membrane as well as a preparation method and application thereof. The preparation method comprises the following steps: preparing silver carp skin collagen; the preparation method comprises the following steps: preparing an epsilon-polylysine / sulfobutyl-beta-cyclodextrin / carvacrol inclusion compound; the preparation method comprises the following steps: dissolving an epsilon-PL / SBE-beta-CD / CAR inclusion compound and silver carp skin collagen in ultrapure water, carrying out a stirring reaction at a certain temperature, adding glycerin, and carrying out stirring so as to obtain a membrane casting solution; and performing ultrasonic degassing on the membrane casting solution, injecting the membrane casting solution into a mold, storing the mold in a constant-temperature and constant-humidity box with the temperature of 26 DEG C and the relative humidity of 50% for 48 hours, and uncovering the membrane to obtain the edible fish collagen-based antibacterial active membrane. The preparation method is simple in technological process, and the prepared edible fish collagen-based antibacterial active film is high in antibacterial performance and good in mechanical performance. The invention provides an effective prevention and treatment strategy for the problem of microbial pollution.

The invention relates to the technical field of intelligent disinfection, and discloses an automatic disinfection microbial culture medium refrigeration generation method and system.The method comprises the steps that data normalization is conducted on multi-source environment data of a refrigeration mechanism, and an environment parameter sequence of the refrigeration mechanism is obtained; according to the environmental parameters in the environmental parameter sequence, performing weighted fusion on the contribution value of the microbial pollution risk in the microbial culture medium to obtain the current pollution risk value of the refrigeration mechanism in the current environment; dynamically adjusting a disinfection trigger threshold value of the refrigeration mechanism according to the accumulated operation time and the historical disinfection frequency of the refrigeration mechanism; when the current pollution risk value continuously exceeds a disinfection trigger threshold value, performing targeted disinfection treatment on the microbial culture medium; after disinfection treatment, recovering and maintaining the preset refrigeration temperature of the microbial culture medium, and returning to the step S3 to carry out closed-loop monitoring on the refrigeration mechanism; the refrigeration and disinfection effects of the microbial culture medium can be improved.

The present invention relates to a composition for controlling, containing and combating pest infestation and microbial contamination on surfaces, comprising an inorganic carrier material comprising silicon dioxide, titanium dioxide, silicates and / or phosphates, and a dye configured to absorb electromagnetic radiation of wavelengths between 400 and 800 nm, in particular between 500 and 700 nm.

The utility model relates to the technical field of food processing, and discloses a crushing and grinding device for food processing, which comprises a feed hopper, an ultraviolet lamp, a discharge hopper, a second electrically operated gate, a rolling device, a grinding device, a screening device, a stirring device and a pushing device. The cabinet body is arranged on the four supporting frames, the feeding hopper is arranged at the top of the cabinet body, the ultraviolet lamp is arranged in the cabinet body, the discharging hopper is arranged at the bottom of the cabinet body, the second electric door is arranged in the discharging hopper, the rolling device is arranged on the cabinet body, the grinding device is arranged on the cabinet body, the screening device is arranged on the cabinet body, and the stirring device is arranged on the cabinet body. The pushing device is arranged on the cabinet body. Due to the closed design and the sterilization effect of the ultraviolet lamp, microbial pollution can be prevented, and pollution of the environment to the raw materials is reduced.

The invention discloses a method for rapidly predicting microbial contamination of meat based on multi-modal fusion. The method comprises the following steps: acquiring spectrum and smell data on the surface of meat, extracting characteristics related to microbial metabolism, and establishing a quantitative relationship between the characteristics and the content of microorganisms (such as the total number of bacterial colonies) by utilizing a machine learning model, thereby realizing rapid and lossless prediction on microbial pollution. According to the method, the defect of long period of a traditional microorganism detection method is overcome, and an effective and rapid monitoring means is provided for microorganism safety risks in the meat production and circulation process.

The invention provides a method for increasing the germination rate of ancient lotus seeds, and belongs to the technical field of ancient lotus seed reactivation, and the method comprises the following steps: selecting seeds, physically breaking seed coats of the selected seeds, soaking the seeds in water for germination after the seed coats are physically broken, controlling physical conditions, adding a nutrient solution, and regularly checking the germination state of the seeds. After the seeds reach the transplanting standard, transplanting the seeds into a matched matrix culture; the germination success rate can reach 82.7 + / -3.5% (n is equal to 300). All operations need to be carried out in a sterile operation table (the cleanliness is ISO 5 level), and microbial contamination is avoided.

This invention provides an integrated device for controlling microbial contamination in seawater desalination, comprising a base, a high-pressure pump mounted on the left side of the base, an online flow cytometer system mounted on the base, and an RO membrane holder mounted on the right side of the base. The base facilitates the installation of the dosing mechanism, diaphragm pump, and online flow cytometer system. The high-pressure pump pressurizes and delivers seawater for subsequent desalination via the RO membrane. The online flow cytometer system monitors the microorganisms in the seawater delivered by the high-pressure pump and simultaneously controls the diaphragm pump to deliver the bactericide from the dosing mechanism upstream of the cartridge filter, sterilizing the seawater entering the RO membrane and thus extending the cleaning cycle and lifespan of the RO membrane.

The invention discloses a high-throughput detection system for microbial contamination in fermented food, and relates to the technical field of fermented food microbial detection, and the high-throughput detection system comprises a sample pretreatment module for performing differential treatment on pickled vegetables, fermented milk and the like and enriching microorganisms; the nucleic acid extraction module is used for extracting by a paramagnetic particle method; the high-throughput amplification module is used for detecting a sample at a time by using a digital PCR instrument; the detection and analysis module is used for laser fluorescence detection; a data storage and management module, an industrial-grade server and a cloud backup; and the quality control module contains a standard nucleic acid library, synchronously performs blank and negative and positive control, and does not pass sound-light alarm. The method is suitable for complex substrates of fermented food, detection is accurate and efficient, and the detection period is shortened; cross-laboratory data synchronization is supported, emergency response is quick, pollution can be quickly positioned, disposal suggestions can be provided, and fermented food quality supervision and risk prevention and control are assisted.

According to the microbial pollution treatment system and method based on the medium-pressure ultraviolet and the hydrogen peroxide, an intelligent cooperative control strategy with the water inlet flow and the ultraviolet light transmittance as feedforward and the oxidation-reduction potential in front of a membrane as core feedback is constructed, the power of a medium-pressure ultraviolet lamp and the adding amount of the hydrogen peroxide are dynamically adjusted in a linkage mode, and the pollution treatment effect of the medium-pressure ultraviolet and the hydrogen peroxide is improved. The maximization of the advanced oxidation synergistic effect and the precise management and control of the membrane safety risk are realized, so that the technical effects of efficiently inhibiting microbial pollution, absolutely guaranteeing the safety of the reverse osmosis membrane, remarkably reducing the operation energy consumption and the medicine consumption and improving the water quality fluctuation resistance of the system are achieved.

The utility model relates to the technical field of medicine detection, and discloses a microorganism quantitative culture medium platform for medicine detection, which comprises an incubator, a culture cavity is arranged on the right side of the inner wall of the incubator, a separation mechanism is arranged on the inner wall of the culture cavity, and a sealing mechanism is arranged on the front side of the outer wall of the culture cavity. An ultraviolet lamp is fixedly connected to the front side of the top of the inner wall of the culture cavity, adjusting mechanisms are arranged on the periphery of the bottom of the culture box, and a controller is fixedly connected to the left side of the inner wall of the culture box. And the separation mechanism comprises a plurality of placing plates. According to the utility model, the placing plate, the heating wire, the temperature detector and the humidity detector are matched with the controller, the temperature and the humidity are accurately regulated and controlled, the microorganism culture condition is met, the culture dish is independently drawn and pulled, and the torque spring and the baffle are matched, so that the culture dish is prevented from falling off and microorganism pollution is avoided; the problem of microorganism cross contamination caused by cover plate movement in the prior art is solved.

The present invention relates to a bio-based microbial retardant composition and a method of preparation thereof The composition comprises lactic acid (2-20% v / v), glutaraldehyde (0.5-10% v / v), Polysorbate 80 (Tween 80) (1-15% v / v), Melia Azadirachta Seed Oil (Neem oil) (0.05-5.5% v / v), and Methylchloroisothiazolinone (Preservative DB20) (0.01-1% v / v). This composition is designed to maintain an acidic pH of 1.5 to 3.5, ensuring immediate microbial retardation. The lactic acid stabilizes the glutaraldehyde, preventing polymerization, while Polysorbate 80 emulsifies Neem oil to enhance antimicrobial action. The preservative inhibits initial microbial contamination, providing immediate suppression of microbial load. The composition is readily biodegradable, exhibiting over 70% degradation within 28 days and is capable of broad-spectrum antimicrobial activity, effective against gram-negative bacteria, fungi, and algae. The composition shows sustained antimicrobial efficacy across multiple generations without inducing resistance. A method of preparing the composition is also provided, involving a sequence of mixing steps for optimal performance.

This invention belongs to the field of water softening and sterilization technology, and discloses an ion-selective adsorption electrode, a micro-electric field adsorption system, and their application in water softening and sterilization / algae removal. An ion-selective adsorption electrode comprises polypolycarboxylate ion exchange fibers, activated carbon, a conductive agent, and a binder, wherein the mass percentage of the polypolycarboxylate ion exchange fibers is 5-20%. This invention uses CCEF-modified activated carbon as the cathode, and at the optimal doping level, it effectively removes Ca... 2+ and Mg 2+ The selectivity coefficients were 15.0 and 13.5, respectively, for Ca 2+ The electroadsorption capacity of the electrode is 311 μmol / g, which is much higher than that of the AC electrode. After 50 cycles of stability testing, the Ca... 2+ The electroadsorption capacity remained above 85%, demonstrating good regeneration ability. Activated carbon modified with nano-silver was used as the anode, and under electric field enhancement, the inhibition rates against bacteria and microalgae reached over 99% and 90%, respectively, exhibiting excellent antimicrobial contamination capabilities.

The utility model relates to cod intestine processing technical field discloses a kind of cod intestine heating cutting integrated machine, including rack, the bottom end of rack is fixedly connected with base, the feeding end of rack is communicated with feeding hopper, the discharge end of rack is communicated with heating box, the bottom end of heating box is provided with discharge port, the inside of rack is provided with for the push cutting mechanism that compact extrusion and equidistant cutting of cod intestine are carried out, the device utilizes push cutting mechanism, equidistantly the compact extrusion of cod intestine can be carried out automatic cutting, ensure that the length of every section cod intestine is close to consistent, realize the standardization of product specification, this not only improves the aesthetic degree and efficiency of packaging link, makes subsequent automatic metering, bagging process smooth and unobstructed, more reduces inherent length error of manual cutting, uneven section and the problem of low efficiency, reduces material loss, simultaneously avoids the microbial contamination risk of personnel frequent contact food, and prevents the safety hazard caused by the mis-cutting of cutting tool.

The present invention relates to a method for hygienizing, disinfecting or sterilizing microbiologically contaminated aqueous fluids, comprising treating the microbiologically contaminated aqueous fluid with an oxidizing agent, wherein a fluorescence measurement is performed on the aqueous fluid before, after and / or during the treatment of the microbiologically contaminated aqueous fluid with the oxidizing agent, which comprises irradiation with an excitation wavelength characteristic of a fluorescent protein and / or peptide building block and measuring the corresponding fluorescence signal, characterized in that the treatment with the oxidizing agent is carried out in such a way that the addition of the oxidizing agent is controlled depending on the fluorescence signal.The present invention further relates to a corresponding device for the hygienization, disinfection or sterilization of microbiologically contaminated aqueous fluid.

The present invention discloses a wet type cooked beef and beefsteak production process, and relates to the technical field of high-end production of beef. Compared with the prior art, the scheme has the advantages that superior raw material screening is more accurate, A4 / A5 and cattle are adopted, IMF and pH values are detected through filing, and it is ensured that the initial quality is controllable; acid discharge and ripening environment parameters are digitized, the temperature fluctuation is less than 0.5 DEG C, the humidity is accurate to 5%, and the quality fluctuation caused by the fact that a traditional process depends on experience is avoided; the residual oxygen content in high-vacuum packaging is less than or equal to 0.5%, and the microbial pollution risk is reduced by 80% in combination with metal detection; the stacking spacing and the number of layers are strictly controlled, the ripening uniformity is improved by 40% in cooperation with an air circulation system, and standardized production is achieved through regular sampling inspection of the pH value and tenderness. Through the improvements, the tenderness of the beef steak is improved by 30%, the accumulation of flavor substances is increased by 25%, the shelf life is prolonged to 45 days, and meanwhile, the processing loss rate is reduced to 3% or below.

The invention discloses a pemetrexed disodium liquid composition as well as a preparation method and application thereof. The pemetrexed disodium liquid composition provided by the invention comprises a pharmaceutical active ingredient and a stabilizer, the active pharmaceutical ingredient is one or more of pemetrexed disodium, a pharmaceutically acceptable complex of the pemetrexed disodium, salt, a solvate and a hydrate; the stabilizer is one or more of an organic solvent, a pH regulator and an antioxidant. The pemetrexed disodium liquid composition disclosed by the invention has the advantages of simple preparation process, good physical and chemical stability, convenience in clinical use, no need of redissolution, low microbial pollution risk in the preparation process, suitability for industrial production and the like.

The invention provides a method for high-pressure propagation and rapid seedling culture of acanthopanax giraldii harms, and belongs to the technical field of rapid propagation of acanthopanax giraldii harms. The method comprises the following steps: 1-3 days before high-pressure propagation in April to August, shading seed trees of acanthopanax giraldii harms; the method comprises the following steps: selecting branches which grow for more than or equal to 1 year on an acanthopanax giraldii harms seed tree, girdling, and scraping away phloem of the girdled part; carbendazim, a rooting solution 1 and a rooting substrate are mixed and filled into a breeding box, the breeding box is pressed at the branch girdling position, a girdling notch is kept in the dead center of the breeding box, and the breeding box is fixed; the relative humidity of the rooting substrate in the breeding box is kept to be 70%-80%, and a rooting promoting nutrient solution is supplemented regularly. According to the method, the problems of microbial contamination and browning in traditional tissue culture are solved, only 4-6 months are needed, the seedling culture time of the acanthopanax giraldii harms is greatly shortened, and the reproduction rate and the branch utilization rate are remarkably increased; the rooting rate and the survival rate both reach 90% or above, and the material cost and the time cost are saved.