107 results about "Microbiological contamination" patented technology

The invention provides a puree production line microbial pollution risk real-time monitoring and early warning method, which comprises the following steps: deploying multiple types of sensors, an industrial camera and an operation log interface, collecting environmental temperature and humidity, pH value, dissolved oxygen, image and operation behavior multi-modal data in a fermentation tank in real time, and carrying out edge calculation and standardized preprocessing to obtain the microbial pollution risk of a puree production line. Data missing, abnormity and time sequence difference are eliminated; multi-modal features and a dynamic knowledge graph constructed based on a production process and a pollution event are fused, and a deep learning model and a graph neural network are utilized to realize microbial pollution risk probability intelligent prediction; risk grading and a dynamic weighting algorithm are introduced, grading early warning signals and disposal suggestions are automatically generated, and a production control system is linked to execute response measures; according to the subsequent production state, continuous feedback is carried out, the model and the early warning threshold are adjusted in a self-adaptive mode, and the accuracy, response efficiency and production safety of pollution early warning are remarkably improved.

The invention discloses a food safety risk assessment method and system based on model analysis, and relates to the technical field of food safety production monitoring, a production line virtual model synchronized with a physical production line in real time is constructed based on the structural parameters of a target production line, and the deviation of production line data is corrected through a model fusion algorithm, so that the risk assessment accuracy is improved. And identifying abnormal physical parameter inflection points strongly related to microbial pollution. According to the method, a production line virtual model is constructed through digital twinning, a model fusion algorithm is introduced, time-domain and frequency-domain multi-dimensional comparison and correction are performed on real-time misalignment data and historical steady-state data, instantaneous interference and systematic drifting are effectively filtered out, the corrected production line data are output, and the real-time misalignment data and the historical steady-state data are compared and corrected. And on the basis, abnormal physical parameter inflection points strongly related to microbial pollution are accurately identified, so that weak abnormal signals which are difficult to perceive by a traditional method can be captured in the initial stage of continuous production, and the early pollution risk perception capability for pathogenic bacteria is remarkably improved.

This invention relates to a method for detecting colorectal cancer tumor cell-associated microorganisms, specifically a method for eliminating intestinal fecal microbial contamination and detecting colorectal cancer tumor-associated microorganisms, belonging to the field of biotechnology. This invention initially eliminates fecal microbial contamination from the sample structure by coarsely shearing and enzymatically digesting the intestinal folds and villi; then, it uses a 40μm cell sieve to filter out incompletely digested tissue fragments, further eliminating intestinal fecal bacteria contamination, and collecting a single-cell suspension; subsequently, the collected single-cell suspension is repeatedly washed with 300g centrifugation to remove as many intercellular or loosely bound "transient bacteria" as possible, retaining intracellular and firmly colonized microorganisms. Ultimately, this eliminates the severe contamination from the large and complex fecal microorganisms, allowing the detection of tumor cell-associated microorganisms, which are already present in low biomass. This method is suitable for detecting microorganisms in gastrointestinal tissues that are in direct contact with the environment.

The invention relates to the technical field of seedling raising, and discloses an efficient tissue culture and rapid propagation seedling raising method for xanthoceras sorbifolia bunge, which comprises the following steps: selecting and pretreating explants, selecting current-year robust branches of the xanthoceras sorbifolia bunge, cutting stem segments with axillary buds as the explants, cleaning, trimming and disinfecting the explants, performing surface disinfection on the pretreated explants, and performing tissue culture and rapid propagation seedling raising on the pretreated explants to obtain the xanthoceras sorbifolia bunge. When the Xanthoceras sorbifolia Bunge is subjected to efficient tissue culture and rapid propagation seedling culture, an operation standard considering thorough sterilization and tissue activity protection is established by standardizing an explant selection standard and executing a step-by-step surface disinfection procedure, so that the microbial contamination rate caused by incomplete disinfection of the explant can be reduced, meanwhile, tissue activity damage caused by excessive disinfection is reduced, and the survival rate of the Xanthoceras sorbifolia Bunge is improved. Therefore, sufficient and healthy sterile initial materials are guaranteed to be obtained, the problems of high primary culture pollution rate and induction starting failure are solved, and a reliable foundation is laid for establishment of a whole tissue culture and rapid propagation system.

The present invention relates to a method for sanitising, disinfecting or sterilising microbially contaminated water-containing fluids, comprising treating the microbially contaminated water-containing fluid with an oxidising agent, wherein a fluorescence measurement is carried out on the water-containing fluid before, after and / or during treatment of the microbially contaminated water-containing fluid with the oxidising agent. The fluorescence measurement comprises irradiation with an excitation wavelength characteristic of a fluorescent protein and / or peptide building block and measurement of the corresponding fluorescence signal, and the method is characterised in that treatment with the oxidising agent proceeds in such a way that addition of the oxidising agent is controlled as a function of the fluorescence signal. The invention further relates to a corresponding device for sanitising, disinfecting or sterilising microbially contaminated water-containing fluid.

A water recycle system may be implemented to analyze and selectively recycle wastewater generated by an industrial textile washer. In some examples, wastewater generated by the industrial textile washer undergoes various treatment processes to generate a clarified wastewater that is stored before being recycled for reuse in the textile washer. Microbial contamination can grow in this stored water over time and the water can become odorous, which is undesirable when subsequently reusing the water to wash textiles. Systems and techniques can be implemented to treat the clarified water to control odor and / or microbial contamination, improving the effectiveness and quality of the water recycle system.

The present invention relates to a composition for controlling, containing and combating pest infestation and microbial contamination on surfaces, comprising an inorganic carrier material comprising silicon dioxide, titanium dioxide, silicates and / or phosphates, and a dye configured to absorb electromagnetic radiation of wavelengths between 400 and 800 nm, in particular between 500 and 700 nm.

The invention discloses a method for rapidly predicting microbial contamination of meat based on multi-modal fusion. The method comprises the following steps: acquiring spectrum and smell data on the surface of meat, extracting characteristics related to microbial metabolism, and establishing a quantitative relationship between the characteristics and the content of microorganisms (such as the total number of bacterial colonies) by utilizing a machine learning model, thereby realizing rapid and lossless prediction on microbial pollution. According to the method, the defect of long period of a traditional microorganism detection method is overcome, and an effective and rapid monitoring means is provided for microorganism safety risks in the meat production and circulation process.

The invention provides a method for increasing the germination rate of ancient lotus seeds, and belongs to the technical field of ancient lotus seed reactivation, and the method comprises the following steps: selecting seeds, physically breaking seed coats of the selected seeds, soaking the seeds in water for germination after the seed coats are physically broken, controlling physical conditions, adding a nutrient solution, and regularly checking the germination state of the seeds. After the seeds reach the transplanting standard, transplanting the seeds into a matched matrix culture; the germination success rate can reach 82.7 + / -3.5% (n is equal to 300). All operations need to be carried out in a sterile operation table (the cleanliness is ISO 5 level), and microbial contamination is avoided.

This invention provides an integrated device for controlling microbial contamination in seawater desalination, comprising a base, a high-pressure pump mounted on the left side of the base, an online flow cytometer system mounted on the base, and an RO membrane holder mounted on the right side of the base. The base facilitates the installation of the dosing mechanism, diaphragm pump, and online flow cytometer system. The high-pressure pump pressurizes and delivers seawater for subsequent desalination via the RO membrane. The online flow cytometer system monitors the microorganisms in the seawater delivered by the high-pressure pump and simultaneously controls the diaphragm pump to deliver the bactericide from the dosing mechanism upstream of the cartridge filter, sterilizing the seawater entering the RO membrane and thus extending the cleaning cycle and lifespan of the RO membrane.

The invention discloses a high-throughput detection system for microbial contamination in fermented food, and relates to the technical field of fermented food microbial detection, and the high-throughput detection system comprises a sample pretreatment module for performing differential treatment on pickled vegetables, fermented milk and the like and enriching microorganisms; the nucleic acid extraction module is used for extracting by a paramagnetic particle method; the high-throughput amplification module is used for detecting a sample at a time by using a digital PCR instrument; the detection and analysis module is used for laser fluorescence detection; a data storage and management module, an industrial-grade server and a cloud backup; and the quality control module contains a standard nucleic acid library, synchronously performs blank and negative and positive control, and does not pass sound-light alarm. The method is suitable for complex substrates of fermented food, detection is accurate and efficient, and the detection period is shortened; cross-laboratory data synchronization is supported, emergency response is quick, pollution can be quickly positioned, disposal suggestions can be provided, and fermented food quality supervision and risk prevention and control are assisted.

According to the microbial pollution treatment system and method based on the medium-pressure ultraviolet and the hydrogen peroxide, an intelligent cooperative control strategy with the water inlet flow and the ultraviolet light transmittance as feedforward and the oxidation-reduction potential in front of a membrane as core feedback is constructed, the power of a medium-pressure ultraviolet lamp and the adding amount of the hydrogen peroxide are dynamically adjusted in a linkage mode, and the pollution treatment effect of the medium-pressure ultraviolet and the hydrogen peroxide is improved. The maximization of the advanced oxidation synergistic effect and the precise management and control of the membrane safety risk are realized, so that the technical effects of efficiently inhibiting microbial pollution, absolutely guaranteeing the safety of the reverse osmosis membrane, remarkably reducing the operation energy consumption and the medicine consumption and improving the water quality fluctuation resistance of the system are achieved.

The utility model relates to the technical field of medicine detection, and discloses a microorganism quantitative culture medium platform for medicine detection, which comprises an incubator, a culture cavity is arranged on the right side of the inner wall of the incubator, a separation mechanism is arranged on the inner wall of the culture cavity, and a sealing mechanism is arranged on the front side of the outer wall of the culture cavity. An ultraviolet lamp is fixedly connected to the front side of the top of the inner wall of the culture cavity, adjusting mechanisms are arranged on the periphery of the bottom of the culture box, and a controller is fixedly connected to the left side of the inner wall of the culture box. And the separation mechanism comprises a plurality of placing plates. According to the utility model, the placing plate, the heating wire, the temperature detector and the humidity detector are matched with the controller, the temperature and the humidity are accurately regulated and controlled, the microorganism culture condition is met, the culture dish is independently drawn and pulled, and the torque spring and the baffle are matched, so that the culture dish is prevented from falling off and microorganism pollution is avoided; the problem of microorganism cross contamination caused by cover plate movement in the prior art is solved.

This invention belongs to the field of water softening and sterilization technology, and discloses an ion-selective adsorption electrode, a micro-electric field adsorption system, and their application in water softening and sterilization / algae removal. An ion-selective adsorption electrode comprises polypolycarboxylate ion exchange fibers, activated carbon, a conductive agent, and a binder, wherein the mass percentage of the polypolycarboxylate ion exchange fibers is 5-20%. This invention uses CCEF-modified activated carbon as the cathode, and at the optimal doping level, it effectively removes Ca... 2+ and Mg 2+ The selectivity coefficients were 15.0 and 13.5, respectively, for Ca 2+ The electroadsorption capacity of the electrode is 311 μmol / g, which is much higher than that of the AC electrode. After 50 cycles of stability testing, the Ca... 2+ The electroadsorption capacity remained above 85%, demonstrating good regeneration ability. Activated carbon modified with nano-silver was used as the anode, and under electric field enhancement, the inhibition rates against bacteria and microalgae reached over 99% and 90%, respectively, exhibiting excellent antimicrobial contamination capabilities.

The present invention relates to a method for hygienizing, disinfecting or sterilizing microbiologically contaminated aqueous fluids, comprising treating the microbiologically contaminated aqueous fluid with an oxidizing agent, wherein a fluorescence measurement is performed on the aqueous fluid before, after and / or during the treatment of the microbiologically contaminated aqueous fluid with the oxidizing agent, which comprises irradiation with an excitation wavelength characteristic of a fluorescent protein and / or peptide building block and measuring the corresponding fluorescence signal, characterized in that the treatment with the oxidizing agent is carried out in such a way that the addition of the oxidizing agent is controlled depending on the fluorescence signal.The present invention further relates to a corresponding device for the hygienization, disinfection or sterilization of microbiologically contaminated aqueous fluid.

The invention provides a method for high-pressure propagation and rapid seedling culture of acanthopanax giraldii harms, and belongs to the technical field of rapid propagation of acanthopanax giraldii harms. The method comprises the following steps: 1-3 days before high-pressure propagation in April to August, shading seed trees of acanthopanax giraldii harms; the method comprises the following steps: selecting branches which grow for more than or equal to 1 year on an acanthopanax giraldii harms seed tree, girdling, and scraping away phloem of the girdled part; carbendazim, a rooting solution 1 and a rooting substrate are mixed and filled into a breeding box, the breeding box is pressed at the branch girdling position, a girdling notch is kept in the dead center of the breeding box, and the breeding box is fixed; the relative humidity of the rooting substrate in the breeding box is kept to be 70%-80%, and a rooting promoting nutrient solution is supplemented regularly. According to the method, the problems of microbial contamination and browning in traditional tissue culture are solved, only 4-6 months are needed, the seedling culture time of the acanthopanax giraldii harms is greatly shortened, and the reproduction rate and the branch utilization rate are remarkably increased; the rooting rate and the survival rate both reach 90% or above, and the material cost and the time cost are saved.

The application discloses a mandelonitrile-degrading bacterium SYY40 and a culture method and application thereof, relates to the technical field of microbial pollution degradation and environmental pollution, and provides the mandelonitrile-degrading bacterium SYY40 which is identified as Pseudomonas sp. SYY40 and named Pseudomonas sp. SYY40, and is preserved in the China General Microbiological Culture Collection Center on May 19, 2025, and the preservation number of the strain is CGMCC NO. 34596. The strain can realize efficient removal of mandelonitrile, and the degradation rate of the mandelonitrile reaches 100% within 48 hours.

A tray laying machine for moist heat sterilization of medicine powder comprises a rack, and a sterilization tray placing device, a sterilization tray conveying device and a charging tray laying device are arranged on the rack. Automatic medicine powder charging and tray laying are achieved through the sterilization tray containing device, the sterilization tray conveying device and the charging and tray laying device, closed conveying and accurate operation are achieved through automatic connection, the links of temporary medicine powder storage and manual scooping are omitted, the medicine powder splashing loss and the microbial contamination risk are remarkably reduced, meanwhile, the charging uniformity and the tray laying efficiency are improved, and the labor intensity of workers is lowered. The labor cost and the labor intensity are reduced, and the large-scale production requirement is met.

The invention provides a microbial pollution source tracing method based on Sanger sequencing overlapping peak analysis, which comprises the following steps: analyzing and enumerating multi-base overlapping peaks in a sequencing peak graph to generate all possible base combination sequences, comparing with a microbial database, and screening effective sequences to identify bacterial strain types. And high-threshold homologous comparison is further carried out on the identified mixed strains and a pre-constructed pollution source standard gene sequence library, so that the pollution source is accurately traced. According to the method, original invalid sequencing data is fully utilized, repeated experiments are avoided, and accurate identification of main strains and systematic tracing of pollution sources are realized.

The invention relates to the technical field of environment intelligent detection, in particular to an intelligent detection device for a human settlement environment, and the device comprises an area module which is used for monitoring the environment parameters of a plurality of main areas; the special module is used for dividing the area for placing the shoes in the main area into independent auxiliary areas, and dividing the auxiliary areas into a plurality of independent sub-areas suitable for placing the shoes; after the shoes are placed in a certain sub-area, detecting air environment parameters above the corresponding sub-area; the processing module is used for judging the pollution level of the main area and the pollution level of the auxiliary area, and generating suggestion instructions of the main area and the auxiliary area respectively. According to the invention, the special monitoring of the shoe cabinet area is pertinently set, all-directional coverage monitoring of the conventional environment of the main area and the special pollution of the auxiliary shoe placement area is realized, corresponding wearing guidance suggestions are given in advance, a traditional original judgment mode depending on smell is replaced, and the health risk caused by microbial pollution is effectively reduced.

The present application provides a sericin / guar gum / polyvinyl alcohol (PVA) composite gel and its application as a fever-reducing patch. For the first time, sericin, cationic guar gum and PVA are used as a composite matrix, and sodium periodate and copper sulfate are introduced simultaneously. Through the precise assignment and synergistic effect of functional units, the problems of microbial contamination and insufficient high-temperature stability are systematically solved. The composite gel prepared by the present application has the advantages of initial adhesion (instant fixation), environmental stability (resistant to heat 42℃ / resistant to cold 0℃), good sustained cooling effect and skin safe pH (4.5-7.0), and is very suitable for clinical application.

This invention relates to the field of milk storage tanks, and more particularly to a device for monitoring the stoppage of a milk storage tank's stirring shaft. A drive assembly is fixedly mounted on the top of the milk storage tank body. The drive assembly carries a stirring shaft that extends into the milk storage tank body. A fixed base is fixedly mounted on the top of the stirring shaft, and an observation tube is fixedly mounted on one side of the fixed base. Multiple movable parts are guided on the outer side of the fixed base, and multiple counterweights are vertically movably mounted inside the fixed base. Each movable part is connected to a counterweight via a traction rope, and an identification flag is fixedly mounted on the top of the fixed base. In this invention, the drive assembly drives the stirring shaft to stir within the milk storage tank body. The user can determine whether the stirring shaft has stopped by observing the identification flag and the observation tube, thus achieving continuous monitoring of the stirring shaft. This device does not come into direct contact with the liquid, preventing the risk of microbial contamination and ensuring its service life. Furthermore, this device is inexpensive.

Provided are innovative compositions for tethering blocking an inactivating of airborne respiratory infectious viruses. The compositions comprise bispecific proteins with two different antigen binding regions (ABR), which are typically configured as immunoglobulin “single variable domains” (ISV). A first ISV binds to a surface protein found on an airborne infectious virus. A second ISV binds to a mucin protein, e.g. a mucin protein present on ocular, nasopharyngeal, tracheal and / or oral surfaces of a mammal. The two ISV are joined by a polypeptide linker.

This disclosure provides methods of using an eco-friendly colloidal silver-based composition in preventing and reducing contamination caused by microorganisms in the plant tissue culture processes. The composition comprises colloidal silver, a copolymer, surfactant, a base, and water. The methods allow cultivating plants or explants in a plant tissue culture medium under non-aseptic conditions and thus obviate the need of the cumbersome and laborious procedures to sterilize the culture medium and the culture vessel in order to create aseptic conditions for plant or explant cultivation.

The invention discloses an RNA helicase inhibitor purification process based on solvent extraction, and relates to the technical field of RNA helicase inhibitors, the RNA helicase inhibitor purification process comprises a shell, an extractor, a washer, a first motor, a first delivery pipe, a first discharge pipe, a first rotating rod, a first stirring impeller and a liquid pump; the extractor is fixedly connected to the interior of the shell; the washer is fixedly connected to the right side of the extractor in the shell; an extraction function and a washing function are integrated in the same device through an integrated structural design, and an extracted solvent phase containing a target object can directly enter a washing link in the device without being transferred, so that the problems of microbial pollution, target object activity loss and residue loss caused by a transfer process in a traditional step-by-step process are effectively avoided; the purification yield and the product purity are obviously improved; the operation process is greatly simplified, the production cycle is shortened, the process complexity and the production cost are reduced, and the stability and the practicability of the RNA helicase inhibitor purification process are integrally improved.

The present application relates to the technical field of disinfection device, particularly relates to a kind of storage tank disinfection device for milk processing, including milk tank, the top of milk tank is fixed with the sealing cover for sealing it, the bottom of milk tank is provided with the base for supporting it, the outside of milk tank is also provided with the hot steam sterilizer for disinfecting its inside, the outside of milk tank is also provided with the adjusting mechanism for driving it to overturn;The present application is cooperated by adjusting mechanism, tank mouth wiping mechanism, servo motor, transmission rod, disinfection block, mounting plate and adjusting disc and other mechanisms, drives hot steam sterilizer and disinfection block to carry out orderly rotation operation, by rotation switching to disinfection block, the structure dead angle that traditional disinfection scheme cannot reach, such as accurate covering tank mouth outer surface, thread joint, sealing rubber ring fitting gap, can prevent secondary microbial contamination of milk in disinfected tank from the source.

The invention relates to a fresh-cut lotus root fresh-keeping method and a compound fresh-keeping agent and a fresh-keeping product thereof, and belongs to the technical field of food processing and fresh keeping. According to the method, in order to solve the problem of quality deterioration caused by easy enzymatic browning and microbial contamination in the storage process of the fresh-cut lotus roots, the fresh-cut lotus root slices are sequentially subjected to active electrolyzed water soaking treatment and sodium fumarate solution soaking treatment in a synergistic manner, so that the browning key enzyme activity and microbial growth are effectively inhibited. The method is simple and convenient to operate, can obviously delay the browning process of the fresh-cut lotus roots and reduce the microbial load, so that the appearance color and sensory quality of the fresh-cut lotus roots are maintained, the shelf life is prolonged, and the method is suitable for postharvest fresh-keeping treatment of the fresh-cut lotus roots.

The utility model relates to the technical field of microorganism culture, in particular to a microorganism culture device which comprises a culture box and an upper cover, a stirring shaft is arranged on the upper cover, the lower end of the stirring shaft extends into the culture box, the upper end of the stirring shaft penetrates through the upper cover and then is connected with a driving mechanism, and stirring rods are arranged on the outer wall of the stirring shaft in the radial direction; the stirring rod is hollow; an aeration disc is mounted on the stirring rod; a gas cavity is formed in the stirring shaft; the gas cavity communicates with the stirring rod; a rotary gas supply assembly matched with the gas cavity is arranged at the upper end of the stirring shaft. The design of the stirring shaft and the hollow stirring rod is matched with the aeration disc mounted on the stirring rod, so that the aeration function and the stirring function are integrated, and compared with a traditional complicated aeration device, the internal structure of the incubator is simplified, the cleaning difficulty is reduced, and the nutrient solution residue and microbial pollution risk are reduced.

The present invention relates to a cell culture vessel characterized in that it comprises an inner surface on which one or more lectins are immobilized, the lectins being capable of binding to one or more species of microorganisms. Furthermore, the invention relates to a method for cultivating cells, comprising bringing the cell culture medium containing the cells into contact with a surface bearing immobilized lectin, and to the use of immobilized lectin to reduce microbial contamination in a cell culture brought into contact with such a surface.

Method of reducing the microbial load on a surface contaminated with microbes, the method comprising (a) identifying a surface in need of microbial reduction; and (b) applying an effective amount of an antimicrobial composition to the surface for a contact time and at a temperature sufficient to reduce the microbial load by at least 50%, wherein the antimicrobial composition comprises:(i) an effective amount of an antimicrobial agent consisting of at least one compound according to Formula 1:wherein R1 is a branched or unbranched, saturated or unsaturated, unsubstituted C3 to C5 alkyl chain or a C3 alkyl chain substituted with a methoxy group; and(ii) a diluent, q.s. to 100;and wherein the antimicrobial composition is substantially free of peroxygen compounds, antibiotics, and chloroacetamide.