ABO, RhD blood typing reagent card, preparation method thereof and antibody diluting solution adopted in preparation method

A technology of antibody diluent and reagent card, which is applied in biological testing, material inspection products, measuring devices, etc., and can solve problems such as inconvenient use, reduced accuracy of ABO blood typing, and large gaps

Active Publication Date: 2012-05-09
苏州苏大赛尔免疫生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0023] At home and abroad, some manufacturers have already produced ABO/RhD blood typing detection reagent cards for ABO blood typing and RhD typing according to the above-mentioned method, but there are two following deficiencies: on the one hand, the gel it adopts is dextran gel , the particles are larger, and the gaps between the gels are larger, which reduces the sensitivity of ABO blood typing; on the other hand, the medium of the suspensio

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] A preparation method for ABO, RhD blood typing reagent card, is characterized in that, comprises the following steps:

[0051] Step 1, the preparation of the antibody diluent, the formula of the antibody diluent is as follows:

[0052] Sodium chloride: 1.5g / L, glycine: 20g / L, disodium hydrogen phosphate: 0.30g / L, sodium dihydrogen phosphate: 0.50g / L, bovine serum albumin: 12g / L, sodium azide: 0.015g / L, Tween 80: 0.005g / L, add water for injection to 1L;

[0053] Step 2: Gel pretreatment

[0054] a. Moist heat sterilization: select propylene dextran gel, add physiological saline suspension gel according to the mass volume ratio of 1:1.2, carry out moist heat sterilization, and set aside after moist heat sterilization;

[0055] b. Gel washing: After damp heat sterilization, add the antibody diluent to the gel according to the mass volume ratio of 1:2, suspend the gel, and let the gel stand for 6 hours to allow the gel to settle naturally and become firm, then pour off the...

Embodiment 2

[0065] A preparation method for ABO, RhD blood typing reagent card, is characterized in that, comprises the following steps:

[0066] Step 1, the preparation of the antibody diluent, the formula of the antibody diluent is as follows:

[0067] Sodium chloride: 2.0g / L, glycine: 10g / L, disodium hydrogen phosphate: 0.50g / L, sodium dihydrogen phosphate: 0.30g / L, bovine serum albumin: 20g / L, sodium azide: 0.005g / L, Tween 80: 0.015g / L, add water for injection to 1L;

[0068] Step 2: Gel pretreatment

[0069] a. Moist heat sterilization: select propylene dextran gel, add physiological saline suspension gel according to the mass volume ratio of 1:2, carry out moist heat sterilization, and use it for later use after moist heat sterilization;

[0070] b. Gel washing: After damp heat sterilization, add the antibody diluent to the gel according to the mass volume ratio of 1:1.2, suspend the gel, and let the gel stand for 7 hours so that the gel can settle naturally and become firm, and ...

Embodiment 3

[0080] A preparation method for ABO, RhD blood typing reagent card, is characterized in that, comprises the following steps:

[0081] Step 1, the preparation of antibody diluent, the formula of described antibody diluent is as follows: sodium chloride: 1.8g / L, glycine: 15g / L, disodium hydrogen phosphate: 0.40g / L, sodium dihydrogen phosphate: 0.45g / L L, bovine serum albumin: 17g / L, sodium azide: 0.010g / L, Tween 80: 0.011g / L, add water for injection to 1L;

[0082] Step 2: Gel pretreatment

[0083] a. Moist heat sterilization: select propylene dextran gel, add physiological saline suspension gel according to the mass volume ratio of 1:1.8, carry out moist heat sterilization, and set aside after moist heat sterilization;

[0084] b. Gel washing: After damp heat sterilization, add the antibody diluent to the gel according to the mass volume ratio of 1:1.7, suspend the gel, and let the gel stand for 8 hours, so that the gel can settle naturally and become firm, and pour off the su...

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Abstract

The invention discloses an ABO, RhD blood typing reagent card, a preparation method thereof and an antibody diluting solution adopted in the preparation method. In the preparation method, the pH value of a system is maintained by virtue of a sodium phosphate buffer system, tween 80 is added, the component of a gel preservation solution is removed, and proper lubrication capability can be obtained when erythrocyte passes through the gap of gel, so that unaggregated erythrocytes have the capability of passing through gaps of gel particles, and aggregated erythrocytes cannot pass; nonspecific adsorption of the gel is eliminated by adding bovine serum albumin to protect the titer of the antibody; microbial contamination is reduced by adopting sodium azide, the preservation period of the product is prolonged, and blood typing error caused by microbial contamination is eliminated; and the gel is screened in the preparation process, and the gel raw material which is obtained by virtue of screening is required to be swelled, wet heat sterilization and cleaning, so that microbes contained in the gel are eliminated, the components of the gel preservation solution and broken gel particles are eliminated, and the product quality is greatly improved.

Description

technical field [0001] The invention relates to the field of blood typing test, in particular to an ABO and RhD blood typing reagent card, a preparation method thereof and an antibody diluent used therefor. Background technique [0002] In clinical treatment, blood transfusion is an important means and measure to save the lives of the wounded and sick. In order to ensure the safety of blood transfusion, it is very important to choose reliable experimental methods and adopt experimental techniques with high specificity and sensitivity. [0003] Blood type is one of the main characteristics of human blood, expressing the genetic traits of various blood component antigens, among which ABO blood type and RhD blood type have become important blood group systems in blood transfusion and organ transplantation. [0004] As early as 1907, Hoktoen first proposed the importance of blood group testing for blood transfusion therapy. In 1908, Ottenberg et al. further proposed the concept...

Claims

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Application Information

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IPC IPC(8): G01N33/80G01N33/531
Inventor 费敏
Owner 苏州苏大赛尔免疫生物技术有限公司
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