Quantum dot immunochromatography detection card and detection method for detecting ibuprofen by double antibody sandwich immunocompetitive method

Abstract

The invention relates to a quantum dot immunochromatographic detection card and a detection method for detecting ibuprofen by a double-antibody sandwich immunological competition method. The detection card includes a liner, a detection antigen release pad, a quantum dot probe release pad, a chromatographic membrane and a water-absorbent pad; the detection antigen release pad contains a detection antigen coupled by ovalbumin and ibuprofen; the quantum dot probe The needle release pad contains quantum dot probes labeled with anti-ovalbumin antibody; the chromatographic membrane is provided with a detection line and a quality control line, the detection line is fixed with anti-ibuprofen antibody, and the quality control line is fixed with ovalbumin antigen. The detection antigen dissolves into the detection system from the release pad, forms a double-antibody sandwich immune complex with the detection antibody and the labeled antibody, and generates a fluorescent detection signal. Under the action of the detection reference of the quality control line, it is judged whether the sample contains ibuprofen according to the fluorescent signal on the detection line, which increases the signal strength and stability of the detection, and improves the detection sensitivity and quantitative precision.

Description

technical field [0001] The invention relates to the field of food and drug safety detection, in particular to a method of applying immunochemical detection technology to the detection of illegally added chemicals in health care products and Chinese patent medicines, especially the quantum dot immunochromatography for the detection of ibuprofen by the double-antibody sandwich immunocompetition method Test card and test method. Background technique [0002] The chemical name of ibuprofen is 2-methyl-4-(2-methylpropyl)phenylacetic acid, which belongs to non-steroidal anti-inflammatory drugs, has anti-inflammatory, analgesic and antipyretic effects, and is used for rheumatoid arthritis , Adhesive spondylitis, non-inflammatory joint pain, arthritis, non-articular rheumatism, pain caused by non-articular inflammation, various neuralgia, cancer pain, post-traumatic pain and fever caused by various inflammations, etc., but There are certain side effects, which are clinically prescr...

AI Technical Summary

Problems solved by technology

The "binary system immunocompetition method" has the following disadvantages: 1. It is necessary to label the signal substance for each detection antibody separately, so that the signal substance cannot be prepared uniformly; 2. The detection antibody is in the liquid phase and has insufficient stability; 3. The detection signal intensity and less sensitive

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