The invention relates to application of ozonized oil in small surgical incisions or other wound such as mucosal ulcer, diabetic feet, cervical erosion, anal fissure, anus fistula, soft tissue injury,fall damage wound surface, recurrent wound, abscess, acne, arthritis, joint degeneration, cystitis, furunculosis, neurological disorder osteomyelitis, gangrene, ulcus cruris, synovitis, gout, sinusitis, spondylitis, scapulohumeral periarthritis, lumbocrural pain, periostitis and stomatitis. Through the modes of oral administration, intraperitoneal injection, clyster, gavage, intravenous injection,subcutaneous injection, intramuscular injection, external application, drop and suppository, the diseases can be effectively prevented and treated.