The invention discloses a preparation process of eyesight protection paper, which adopts a mixture of waste paper and straws as a raw material and comprises the following specific steps: a, cutting the straws into sheets, removing impurities and dehydrating; b, putting the straws into a solvent and mixing; c, cooking and removing impurities to obtain straw unbleached pulp; d, crushing waste paper and adding hot water to prepare paper pulp; e, performing deinking treatment on the paper pulp in the step d to obtain waste paper pulp; f, uniformly mixing the straw unbleached pulp, the waste paper pulp and a porous filler to form mixed pulp; g, subjecting the mixed slurry to hot grinding dissociation, and taking one part of the ground slurry as a surface slurry; h, adding a light absorbing filler into the rest of the ground slurry, carrying out hot grinding dissociation again, and taking the ground slurry as a core layer slurry; i, manufacturing the eyesight protection paper through a papermaking machine, wherein the eyesight protection paper is formed by compounding an upper layer, a middle layer and a lower layer, the outer paper faces of the upper layer and the lower layer are made of surface slurry, and the middle light absorption layer is made of the core layer slurry. The preparation process is environment-friendly, resources are saved, and the prepared paper can protect eyesight.