Method for extracting and separating anthocyanidin and polysaccharide from blueberry pomace

A technology for separating anthocyanins and blueberry pomace, which is applied in the field of extracting and separating anthocyanins and polysaccharides, can solve the problems of complex process, laborious, difficult large-scale production, etc., and achieves simple process operation, high added value of products, and organic saving. Effects of Reagents

Inactive Publication Date: 2013-06-19
NORTHEAST FORESTRY UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although enzyme-assisted extraction of anthocyanins and polysaccharides from blueberry fruit has been reported, the methods employed are time-consuming, laborious or not suitable for scale-up applications
The anthocyanin products obtained by preliminary extraction are usually of low quality and poor stability, so it is necessary to further separate and purify them
In order to obtain high-purity anthocyanins, it is often necessary to separate and purify twice or even multiple times. The process is relatively complicated, the investment is large, and it is difficult to produce on a large scale, which also makes high-purity anthocyanins extremely expensive.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Take 50g of blueberry pomace, add 300mg of cellulase and 500mL of distilled water adjusted to pH 3.0 with hydrochloric acid, carry out negative pressure cavitation enhanced extraction twice at -0.07MPa negative pressure and 40°C, each time for 15min, filter and combine The filtrate was concentrated under reduced pressure to 1 / 7 of the total volume of the extraction solvent used, and flowed through an NKA-9 macroporous adsorption resin column with a diameter-to-height ratio of 1: 20 for adsorption loading with a flow rate of 3BV / h. The loading volume was 5BV. After adsorption equilibrium, wash the resin column with 3BV of distilled water at a flow rate of 4BV / h, and elute anthocyanins with 6BV of 10% ethanol solution. The unadsorbed part solution and the distilled water washing part were combined with polysaccharide solution, and the eluted part of 10% ethanol solution was collected to obtain anthocyanin solution, concentrated under reduced pressure and freeze-dried to ob...

Embodiment 2

[0021] Take 50g of blueberry pomace, add 200mg of compound enzyme composed of cellulase and pectinase according to the mass ratio of 1:1 and 350mL of distilled water adjusted to pH4. Intensify the extraction by cavitation twice, each time for 10 minutes, filter, combine the filtrates, concentrate under reduced pressure to 1 / 7 of the total volume of the extraction solvent used, and flow through D14 macropores with a diameter-to-height ratio of 1:15 at a flow rate of 1.5BV / h The adsorption resin column was used for adsorption and loading, and the loading volume was 3BV. After adsorption equilibrium, the resin column was washed with 2BV distilled water at a flow rate of 6BV / h, and the anthocyanins were eluted with 5BV 20% ethanol solution. The unadsorbed part solution and the distilled water washing part were combined with polysaccharide solution, and the eluted part of 20% ethanol solution was collected to obtain anthocyanin solution, concentrated under reduced pressure and freez...

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PUM

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Abstract

The invention relates to a method for extracting and separating anthocyanidin and polysaccharide from blueberry pomace. According to the method, blueberry pomace is used as raw material, enzyme-water solution is used as extraction solvent, and negative pressure cavitation strengthening extraction technology and macroporous resin enrichment and separation technology are applied to obtain anthocyanidin and polysaccharide from the blueberry pomace. Content of anthocyanidin in the obtained product is 26%-40%, and content of polysaccharide is 2.6%-13.8%. The method has the advantages that by-products of blueberries are utilized comprehensively, equipment is simple, economization and environmental protection. Further, the method fits with current trend of development and is suitable for industrialization.

Description

technical field [0001] The invention relates to a method for extracting and separating anthocyanins and polysaccharides from blueberry pomace, belonging to the fields of natural products and food. Background technique [0002] Blueberry (Blueberry) is a perennial deciduous or evergreen fruit tree of the Rhododendronaceae Vaccinium genus, and is one of the rare blue foods on earth. The fruit is a small berry, blue-purple when ripe, spherical or oval, with hoarfrost on the skin. It is mainly produced in the Daxinganling and Xiaoxinganling forest areas in my country, especially the central Daxinganling, and it is all pure wild. The fruit is rich in various functional factors such as flavonoids, polyphenols and polysaccharides. It has high health value, so it is popular all over the world. It is one of the five healthy fruits recommended by the World Food and Agriculture Organization. [0003] Anthocyanin is a natural water-soluble pigment belonging to flavonoids. Research ins...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/62C08B37/00
Inventor 付玉杰魏作富高畅王微祖元刚
Owner NORTHEAST FORESTRY UNIVERSITY
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