Preparation method of tetracycline carbon dot fluorescent molecular imprinting material

A carbon dot fluorescence and molecular imprinting technology is applied in the field of preparation of carbon dot molecularly imprinted fluorescent composite materials, which can solve the problems of complex and expensive instruments and equipment, and achieve the effects of easy control of reaction conditions, simple experimental operation and good stability.

Inactive Publication Date: 2019-01-08
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in these methods, complex and expensive instruments and equipment or complex sample pretreatment

Method used

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  • Preparation method of tetracycline carbon dot fluorescent molecular imprinting material
  • Preparation method of tetracycline carbon dot fluorescent molecular imprinting material
  • Preparation method of tetracycline carbon dot fluorescent molecular imprinting material

Examples

Experimental program
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Example Embodiment

[0032] Example 1

[0033] The invention provides a preparation method of tetracycline carbon dot fluorescent molecular imprinted composite material, and the specific preparation route is as follows figure 1 Shown:

[0034] (1) Dissolve 10g citric acid (CA) in 40ml deionized water, add 4.2g magnesium hydroxide (Mg(OH) 2 ) And 5ml of ethylenediamine (EDA), stir until the solution is colorless and clear, and react in a reactor at 200°C for 3 hours. The resulting product is dialyzed for 48h and freeze-dried for 24h through a dialysis bag to obtain high fluorescent carbon dots ( CDs), protected from light, sealed and stored in a refrigerator at 4°C.

[0035] (2) Add 3 mg of carbon dots (CDs) to a solution of 4.5 ml of methanol and 0.5 ml of deionized water, and ultrasonically dissolve and disperse them evenly. Then add 44.443mg of tetracycline (TC), 0.8mmol of methacrylic acid (MAA), sonicate, then add 7mmol of ethylene glycol dimethacrylate (EGDMA) and 0.07mmol of azobisisobutyronitrile...

Example Embodiment

[0042] Example 2

[0043] Study Example 1 to obtain the adsorption performance of the fluorescent composite material (such as Image 6 Shown). Put 1mg of fluorescent molecularly imprinted composite material in a 2ml centrifuge tube, add 2ml of different concentrations (0, 0.05, 5, 20, 40, 60, 80, 100, 140, 180μg L -1 ) TC standard solution, oscillate 3.5h at room temperature, and measure the fluorescence intensity value with a fluorescence spectrophotometer (λ=360nm). At the same time, the adsorption experiment of non-imprinted composite materials for TC was done in parallel. From Image 6 It can be seen that as the TC concentration increases, the fluorescence intensity decreases, and finally keeps the balance gradually. At the same concentration, the fluorescence quenching amount of CDs@MIP is greater than that of CDs@NIP.

Example Embodiment

[0044] Example 3

[0045] Study Example 1 to obtain the adsorption kinetic performance of the fluorescent composite material (such as Figure 7 Shown). Weigh 1mg of fluorescent molecularly imprinted and non-imprinted composite materials into a 2ml centrifuge tube, add 2ml 100μg L -1 The TC standard solution was shaken at room temperature for 0, 30, 60, 90, 120, 150, 180, 210, 240, 270, 300, 330 min, and the fluorescence intensity was measured with a fluorescence spectrophotometer. Such as Figure 7 As shown, the adsorption of the imprinted composite material to the target almost reached equilibrium at 210 min.

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Abstract

The invention provides a preparation method of a tetracycline carbon dot fluorescent molecular imprinting material. The method comprises the following steps: adopting CDs as a carrier, adopting methanol/water as a solvent, and adding tetracycline, methacrylic acid, ethylene glycol dimethacrylate and azodiisobutyronitrile to form the tetracycline carbon dot fluorescent molecular imprinting material. The method has the advantages that green and stable carbon dots are taken as fluorescence detection signals, and are combined with the selectivity of a molecular imprinting technology, so that the fluorescent molecular imprinting composite material CDs@MIP with high selectivity on tetracycline is synthesized; the preparation technology is scientific, the cost is low, the method is simple to operate, and reaction conditions are easy to control; and the prepared tetracycline adsorption function material is applicable to adsorption detection on trace tetracycline in water environment, and has an excellent application prospect.

Description

technical field [0001] The invention belongs to the research field of polymer optical sensing material preparation technology and antibiotic detection method, and in particular relates to a preparation method of a carbon dot molecularly imprinted fluorescent composite material and its application in recognition and detection of tetracycline. Background technique [0002] Tetracyclines (TCs) have been widely used in veterinary medicine because of their good oral absorption capacity, low toxicity, low cost and broad-spectrum antibacterial activity. However, there are certain safety hazards in the residual tetracycline: consumption of animal meat and other products may cause allergic reactions in people, in addition, the residue may also increase the resistance of bacteria to antibiotics, and what is worse, water and soil environments are also will be polluted. Due to the potential toxicity to human health, many countries have restricted the use of tetracyclines and made stric...

Claims

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Application Information

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IPC IPC(8): C08F222/14C08F220/06C09K11/06G01N21/64B01J20/281
CPCB01J20/281C08F222/10C09K11/06C08F222/102G01N21/6428G01N2021/6432C08F220/06
Inventor 方国臻赵悦清胡雪莲赵鹏王硕
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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