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Water-soluble cationic conjugated microporous polymer phosphorescent probe and preparation method thereof

A technology of water-soluble cations and conjugated micropores, which is applied in the field of phosphorescence chemical probes, can solve the problems of unused metal iridium and achieve good phosphorescence emission, long luminescence life, and easy-to-obtain raw materials

Inactive Publication Date: 2012-08-08
SHANGHAI NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, prior art has not used metal iridium for nucleic acid dyes

Method used

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  • Water-soluble cationic conjugated microporous polymer phosphorescent probe and preparation method thereof
  • Water-soluble cationic conjugated microporous polymer phosphorescent probe and preparation method thereof
  • Water-soluble cationic conjugated microporous polymer phosphorescent probe and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] (1) Weigh 0.1mmol IrCl 3 ·3H 2 O and 0.24mmol 2-(4-bromophenyl)pyridine were added to the three-necked flask, and then a mixed solution of 2-ethylene glycol ether and water with a volume ratio of 4:1 was added; then the reaction mixture was heated to 110°C , stirred and reacted for 30 hours; after the reaction was finished, cooled to room temperature, a yellow precipitate was generated; the precipitate was washed with water and ethanol for 3 to 5 times, and dried in vacuo to obtain a bright yellow solid 2-(4-bromophenyl)pyridine iridium Dichloro Bridge: Ir 2 (Brppy) 4 Cl 2 ;

[0032] (2) Weigh 0.1mmol 2-(4-bromophenyl)pyridine iridium iridium dichloro bridge: Ir 2 (Brppy) 4 Cl 2 and 0.24mmol of 3,8-dibromophenanthroline were added to the three-necked flask; 10mL of the mixed solution of dichloromethane and methanol was added, and the volume ratio of dichloromethane and methanol was 2:1; heated to 45°C , stirred and reacted for 26 hours; after the reaction, the r...

Embodiment 2

[0039] Step (1)~(2) is the same as embodiment 1

[0040](3) Weigh 0.1mmol 3,8-dibromophenanthroline iridium 2-(4-bromophenyl)pyridine complex and 0.1mmol AOT, add 0.024mmol bis(1,5-cyclooctadiene) nickel (O); add 0.24mmol 2,2'-bipyridine; add 0.06mL of COD and 50mL of DMF, heat to 80°C, and stir for 48 hours; after the reaction, add 2M hydrochloric acid and the turbidity does not increase, stir for 30min , centrifuged, washed to pH = 7, and freeze-dried to obtain a brown-red solid.

Embodiment 3

[0042] Spectral Measurement of Water-Soluble Cationic Conjugated Microporous Polymer with Phosphorescent Probe

[0043] The product obtained in Example 1 is dissolved in acetonitrile, ethanol, DMF, THF, and the emission spectrum is measured. The experimental results show that under the excitation of 365nm, the maximum emission peak of the material is at 650nm, and the emission wavelength is not affected in different solvents. ,Such as image 3 shown.

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Abstract

The present invention discloses a water-soluble cationic conjugated microporous polymer phosphorescent probe and a preparation method thereof. 2-(4-bromophenyl) pyridine iridium dichloride bridged complex Ir2(Brppy)4Cl2, 3,8-dibromo phenanthroline and 3,8-dibromo phenanthroline iridium 2-(4-bromophenyl) complex are sequentially prepared to prepare an objective polymer. The polymer chemical probe prepared through the above technical scheme has good water solubility and can bind with nucleic acid through electrostatic interaction; the probe has good phosphorescence emission in water and long luminescence lifetime; and a time-resolved fluorescence technique can effectively eliminate interference of the background fluorescence and improve detection sensitivity. The preparation preparation method has the advantages of simple operation and easily available raw materials, and is beneficial to reduce the cost.

Description

technical field [0001] The invention belongs to phosphorescent chemical probe technology, in particular to a water-soluble cationic conjugated microporous polymer phosphorescent probe and a preparation method thereof. Background technique [0002] As important macromolecules in organisms, nucleic acids are the material basis for studying the phenomena and essence of life. Through the study of their physical and chemical properties, replication and transcription, expression and regulation, etc., we can understand the essential laws of life. Therefore, the research on nucleic acid is of great significance. But nucleic acid is invisible, and its research is mainly to observe its shape, size, and expression level with the help of certain instruments, the most common and simplest of which is agarose gel electrophoresis. EB (Ethidium Bromide, ethidium bromide) is the most commonly used nucleic acid dye in the laboratory. It has the characteristics of simplicity, speed, and high s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/06C07F15/00G01N21/64
Inventor 杨红丁亮秦丽洁周治国石少娟王亚培陈冬梅汪娟娟杨仕平
Owner SHANGHAI NORMAL UNIVERSITY
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