The invention discloses a double-layer touch screen, which comprises transparent cover plate glass, a first transparent impressing adhesive layer, a first conductive layer, a second transparent impressing adhesive layer, a second conductive layer and an anti-reflection layer, wherein the first conductive layer is embedded in the first transparent impressing adhesive layer, and comprises a plurality of first bus-bars, and the first bus-bars extend along a first direction, and are formed by intersecting conductive silk threads; and the second conductive layer is embedded in the first transparent impressing adhesive layer, and comprises a plurality of second bus-bars, and the second bus-bars extend along a second direction, and are formed by intersecting conductive silk threads. Compared with the conventional touch screen, the double-layer touch screen has the advantages that indium tin oxide (ITO) is replaced by the first and second conductive layers formed by a conductive material filled in a first groove and a second groove, an additional etching technology is not required by such a process, the light transmittance and the electrical conductivity are ensured, and meanwhile, the material cost is lower, so that the cost can be lowered. The invention also discloses a preparation method for the double-layer touch screen.