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Paracoccus denitrificans and application thereof in treatment of livestock farm wastewater

A technology for denitrifying paracoccus and wastewater treatment, applied in the biological field, can solve problems such as ecological environment and human health threats, and achieve the effects of low production cost, low energy consumption and low investment

Active Publication Date: 2017-08-25
WUHAN KEYUAN BIOLOGICAL DEV CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Antibiotics are widely used in livestock and poultry farming to prevent and treat animal diseases, but only a small part of these antibiotics are used by animals, and most of them are excreted through urine and feces. Breeding wastewater is an important source of antibiotic pollution. Antibiotics in the environment Enrichment poses a threat to the ecological environment and human health

Method used

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  • Paracoccus denitrificans and application thereof in treatment of livestock farm wastewater
  • Paracoccus denitrificans and application thereof in treatment of livestock farm wastewater
  • Paracoccus denitrificans and application thereof in treatment of livestock farm wastewater

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Obtaining of Paracoccus denitrificans TDFQ-1:

[0040] Lake water and farm wastewater were collected from Nanhu Lake, a pig farm in Suizhou, Hubei, and a chicken farm in Anlu, Hubei, with (NH 4 ) 2 SO 4 As the only nitrogen source, enrich and cultivate bacteria that can use ammonia nitrogen as a nitrogen source in YW liquid medium, culture at 30°C, 200rpm for 72 hours, enrich and passage continuously in YW medium for 3 times, spread the culture solution on a plate after dilution, Pick a single colony and inoculate it in YW liquid medium for shaking flask culture, and use the griess reagent color method to screen the strains with strong nitrification ability (see figure 1 ).

[0041] Liquid nitrification medium (YW) formula: (NH 4 ) 2 SO 4 2g, MnSO 4 0.01g, MgSO 4 0.03g, Na 2 HPO 4 0.25g, KH 2 PO 4 0.75g, CaCl 2 0.2g, FeSO 4 0.03g, glycerin 6g, pH7.8.

[0042] Identification of strain 16S

[0043] The 7 isolated strains were identified, and the publ...

Embodiment 2

[0048] Preparation of Paracoccus denitrificans TDFQ-1:

[0049] 1) Growth curve of Paracoccus denitrificans TDFQ-1

[0050] Inoculate the activated Paracoccus denitrificans TDFQ-1 into 200ml NB and 200ml fermentation medium respectively at 1% (V / V) inoculum, culture on a shaker at 30°C and 200rpm, and take samples every 2h to measure OD600 Value, with time as the abscissa, and OD600 value as the ordinate to draw the growth curve, see Figure 4 .

[0051] Table 1 Growth curve data of Paracoccus denitrificans TDFQ-1

[0052]

[0053]

[0054] The growth curve represents the growth characteristics of microorganisms. According to the growth curve of microorganisms, the growth law of microorganisms can be clarified, and the time of reaching the stable period can be determined to determine the appropriate fermentation and cultivation time, which has certain guiding significance for production practice.

[0055] The results showed that in the NB medium, TDFQ-1 entered the lo...

Embodiment 3

[0061] Effect of Paracoccus denitrificans TDFQ-1 on removal of ammonia nitrogen and total nitrogen in YW medium

[0062] Inoculate Paracoccus denitrificans TDFQ-1 in 200ml NB liquid medium, culture at 30°C, 200rpm for 18 hours, centrifuge the fermentation liquid to remove the supernatant, add sterilized single distilled water to wash twice, add sterilized Resuspend in single distilled water, adjust the OD600 value of the bacterial cell concentration to 1.5; inoculate the resuspended seed solution in 200ml YW medium at an inoculum size of 5%, culture at 30°C and 200rpm for 54h, take 2ml of culture solution every 6 hours for determination The content of total nitrogen and ammonia nitrogen in the fermentation broth. Total nitrogen was determined by potassium persulfate oxidation photometry, and ammonia nitrogen was determined by Nessler's reagent photometry.

[0063] Total nitrogen and ammonia nitrogen determination standard curve: Figure 5 and Image 6 , the results are show...

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Abstract

The invention relates to the technical field of biology and particularly relates to paracoccus denitrificans and an application thereof in treatment of livestock farm wastewater. The paracoccus denitrificans TDFQ-1 is collected at the China Center for Type Culture Collection (CCTCC), and the collection number is CCTCC NO:M2017238. The bacterial strain has the advantages of being high in growth speed, high in environmental tolerance and good in growth in an organic culture medium. The paracoccus denitrificans has a heterotrophic nitrification capability and an aerobic denitrification capability, and is capable of reducing harmful substances, such as ammonia nitrogen and nitrate in water, especially removing the ammonia nitrogen in the livestock farm wastewater with relatively high organic matter content and improving the breeding environment. The bacterial strain is capable of efficiently degrading fluoroquinolone antibiotics when treating the wastewater, reducing the pollution of the antibiotics to soil and water sources and protecting the human health.

Description

technical field [0001] The invention relates to the field of biological technology, in particular to the isolation, identification and cultivation method of a denitrifying paracoccus and its application in wastewater treatment of livestock and poultry farms. Background technique [0002] With the development of society and economy, the scale of intensive livestock and poultry breeding is increasing, the production efficiency is improved, and the economic benefits are increased. The contradiction has become increasingly prominent, and the excessive concentration of feces and waste water has brought great pressure to the ecological environment. The livestock and poultry breeding industry is one of the leading industries in my country's agriculture, and the pollution from livestock and poultry breeding has become one of the main causes of agricultural non-point source pollution. A large amount of livestock and poultry breeding wastewater has become a source of pollution. If it...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F103/20C02F101/38C02F101/16
CPCC02F3/34C02F2101/16C02F2101/38C02F2103/20C12N1/20C12N1/205C12R2001/01
Inventor 金梅林杨丽滑亚锋马海军康超
Owner WUHAN KEYUAN BIOLOGICAL DEV CO LTD
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