Method for removing fluoroquinolones antibiotics in poultry and livestock excrement
A technology of fluoroquinolones and antibiotics, which is applied in the field of removal of antibiotics in poultry and animal manure organic fertilizers, can solve the problems of less research on removal methods, large energy consumption, and large floor area, and achieves easy operation and promotion, and low economic cost Effect
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Embodiment 1
[0025] The present invention removes the method for fluoroquinolone antibiotics in poultry manure, and its steps are as follows:
[0026] Step 1: Activate the Cellulomonas flavogenes stored on the LB medium, inoculate 2% of the Cellulomonas flavogenes into the selective medium, and shake continuously for 72 hours at a constant temperature of 60°C.
[0027] LB medium: peptone: 10g; yeast extract: 5g; NaCI: 10g; distilled water 1000ml; PH: 7.0.
[0028] Selective medium: KH 2 PO 4 : 0.5g, NaCI: 0.5g, Peptone: 0.5g, MgCI 2 : 0.5g, (NH 4 ) SO 4 : 0.5g, NH 4 NO 3 : 1.0g, K 2 HPO 4 : 1.5g, distilled water 1000ml, pH 6.8~7.2.
[0029] Step 2: Mix fresh chicken manure with a moisture content of about 65.0% and an organic carbon content of about 20% with sawdust and distilled water to control the C / N ratio at 20:1 and the moisture content between 55% and 60%. , the amount of bacteria agent is about 5% of the volume of the compost material.
[0030] Step 3: The initial materi...
Embodiment 2
[0038] According to the proportioning of materials and the method of operation of Example 1, the experiment was carried out again. The difference between Example 1 and this Example 2 is: the initial concentration of fluoroquinolone antibiotics in the chicken manure is different. Through three parallel tests, the fluoroquinolone antibiotics The degradation effect is shown in Table 2.
[0039]
[0040] Show from above two examples, through adopting the present invention to process fluoroquinolone antibiotics in chicken manure, make the fluoroquinolone antibiotics of 61.09~82.82% in chicken manure be removed, reduce the harm of chicken manure as organic fertilizer, make wherein fluorine The content of quinolone antibiotics reached the safe limit.
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