Harmless treatment method for macrolide and fluoroquinolone antibiotics in livestock and poultry manure
A technology of macrolides and fluoroquinolones, which is applied in the field of solid waste treatment, can solve problems such as the lack of harmless treatment methods for antibiotics, biologically produced drug resistance genes, and ecological toxicity, and achieve good promotion and application value and realize Efficient degradation and the effect of reducing the content of antibiotics
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Embodiment 1
[0038] Experimental research on harmless treatment of tylosin phosphate in pig manure, including the following steps:
[0039] (1) dehydrating the pig manure to a moisture content of 45% by mass;
[0040] (2) Prepare three pig manure samples with different tylosin phosphate contents, among which T1: no macrolide antibiotics; T2: tylosin phosphate added at a concentration of 100mg / kg dry weight; T3: tylosin phosphate The added concentration of dimethicin reaches 200mg / kg dry weight;
[0041] (3) Set the concentration to 10 6 CFU / mL bacterial liquid (obtained by mixing Proteus mirabilis and Enterococcus faecalis at a ratio of 1:1) was mixed with 100 g of the above three pig manure samples, and pre-fermented for 48 hours;
[0042] (4) After mixing 2g of black soldier fly larvae with 100g of the above-mentioned three kinds of pre-fermented pig manure samples, place them in an incubator for harmless treatment. addition of water;
[0043] (5) The harmless treatment process conti...
Embodiment 2
[0045] Experimental research on the harmless treatment of enrofloxacin hydrochloride in pig manure, including the following steps:
[0046] (1) dehydrating the pig manure to a moisture content of 50% by mass;
[0047] (2) Prepare three pig manure samples with different enrofloxacin hydrochloride contents, among which T1: no fluoroquinolone antibiotics; T2: tylosin phosphate added at a concentration of 100mg / kg dry weight; T3: enrofloxacin hydrochloride Star addition concentration reaches 200mg / kg dry weight;
[0048] (3) Set the concentration to 10 6 CFU / mL bacterial liquid (obtained by mixing Proteus mirabilis and Enterococcus faecalis at a ratio of 5:2) was mixed with 100 g of the above three pig manure samples, and then pre-fermented for 48 hours.
[0049] (4) After mixing 2g of black soldier fly larvae with 100g of the above three pig manure samples, place them in an incubator for harmless treatment. During the treatment process, add feces and water depending on the feed i...
Embodiment 3
[0052] Experimental research on harmless treatment of tylosin phosphate in chicken manure, including the following steps:
[0053] (1) dehydrating the chicken manure to a moisture content of 60% by mass;
[0054] (2) Prepare three chicken manure samples with different tylosin phosphate contents, among which T1: no macrolide antibiotics; T2: tylosin phosphate added at a concentration of 100mg / kg dry weight; T3: tylosin phosphate The added concentration of dimethicin reaches 200mg / kg dry weight;
[0055] (3) Set the concentration to 10 6 CFU / mL bacterial liquid (obtained by mixing Proteus mirabilis and Enterococcus faecalis at a ratio of 1:1) was mixed with 100 g of the above three pig manure samples, and then pre-fermented for 48 hours.
[0056] (4) After mixing 1g of black soldier fly larvae with 100g of the above-mentioned three kinds of chicken manure samples, place them in an incubator for harmless treatment. Add to;
[0057] (5) The harmless treatment process continued...
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