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Harmless treatment method for macrolide and fluoroquinolone antibiotics in livestock and poultry manure

A technology of macrolides and fluoroquinolones, which is applied in the field of solid waste treatment, can solve problems such as the lack of harmless treatment methods for antibiotics, biologically produced drug resistance genes, and ecological toxicity, and achieve good promotion and application value and realize Efficient degradation and the effect of reducing the content of antibiotics

Active Publication Date: 2020-12-25
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Once antibiotics enter the environment, they will continue to diffuse in the soil, water, and atmosphere. They mainly undergo a series of biotransformation processes such as adsorption, hydrolysis, photolysis, and microbial degradation in the environment. They will directly produce ecotoxicity to the environment and exist in the environment. Antibiotics may lead to resistance genes in various organisms, and also cause direct or indirect harm to humans
[0006] The prior art lacks a harmless treatment method for antibiotics in poultry manure

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Experimental research on harmless treatment of tylosin phosphate in pig manure, including the following steps:

[0039] (1) dehydrating the pig manure to a moisture content of 45% by mass;

[0040] (2) Prepare three pig manure samples with different tylosin phosphate contents, among which T1: no macrolide antibiotics; T2: tylosin phosphate added at a concentration of 100mg / kg dry weight; T3: tylosin phosphate The added concentration of dimethicin reaches 200mg / kg dry weight;

[0041] (3) Set the concentration to 10 6 CFU / mL bacterial liquid (obtained by mixing Proteus mirabilis and Enterococcus faecalis at a ratio of 1:1) was mixed with 100 g of the above three pig manure samples, and pre-fermented for 48 hours;

[0042] (4) After mixing 2g of black soldier fly larvae with 100g of the above-mentioned three kinds of pre-fermented pig manure samples, place them in an incubator for harmless treatment. addition of water;

[0043] (5) The harmless treatment process conti...

Embodiment 2

[0045] Experimental research on the harmless treatment of enrofloxacin hydrochloride in pig manure, including the following steps:

[0046] (1) dehydrating the pig manure to a moisture content of 50% by mass;

[0047] (2) Prepare three pig manure samples with different enrofloxacin hydrochloride contents, among which T1: no fluoroquinolone antibiotics; T2: tylosin phosphate added at a concentration of 100mg / kg dry weight; T3: enrofloxacin hydrochloride Star addition concentration reaches 200mg / kg dry weight;

[0048] (3) Set the concentration to 10 6 CFU / mL bacterial liquid (obtained by mixing Proteus mirabilis and Enterococcus faecalis at a ratio of 5:2) was mixed with 100 g of the above three pig manure samples, and then pre-fermented for 48 hours.

[0049] (4) After mixing 2g of black soldier fly larvae with 100g of the above three pig manure samples, place them in an incubator for harmless treatment. During the treatment process, add feces and water depending on the feed i...

Embodiment 3

[0052] Experimental research on harmless treatment of tylosin phosphate in chicken manure, including the following steps:

[0053] (1) dehydrating the chicken manure to a moisture content of 60% by mass;

[0054] (2) Prepare three chicken manure samples with different tylosin phosphate contents, among which T1: no macrolide antibiotics; T2: tylosin phosphate added at a concentration of 100mg / kg dry weight; T3: tylosin phosphate The added concentration of dimethicin reaches 200mg / kg dry weight;

[0055] (3) Set the concentration to 10 6 CFU / mL bacterial liquid (obtained by mixing Proteus mirabilis and Enterococcus faecalis at a ratio of 1:1) was mixed with 100 g of the above three pig manure samples, and then pre-fermented for 48 hours.

[0056] (4) After mixing 1g of black soldier fly larvae with 100g of the above-mentioned three kinds of chicken manure samples, place them in an incubator for harmless treatment. Add to;

[0057] (5) The harmless treatment process continued...

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PUM

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Abstract

The invention belongs to the technical field of solid waste treatment, and discloses a harmless treatment method for macrolide and fluoroquinolone antibiotics in livestock manure, which realizes resourceful treatment of the livestock manure by adopting microbial fermentation and hermetia illucens culture, and realizes harmless treatment of the livestock manure by virtue of the biological decomposition and biological conversion effects of microorganisms and hermetia illucens, which realizes efficient degradation of the residual antibiotics, does not need to add any chemical substances in the whole process, does not need to hold various reaction conditions such as temperature, saves the livestock manure treatment cost, reduces the treatment energy consumption, and greatly reduces the antibiotic content of the treated livestock manure, and finally, no waste is generated in the whole livestock and poultry manure treatment process, growth of hermetia illucens is achieved after hermetia illucens is bred, the bred hermetia illucens has certain economic value, good economic benefits can be generated, and therefore the method has good application and popularization value.

Description

technical field [0001] The invention relates to the technical field of solid waste treatment, in particular to a harmless treatment method for macrolides and fluoroquinolone antibiotics in poultry manure. Background technique [0002] With the rapid development of large-scale livestock and poultry breeding in China, the harmless treatment and resource utilization of farm manure are of great significance to reduce environmental pressure. Moreover, the State Council issued the "Opinions on Accelerating the Resource Utilization of Livestock and Poultry Breeding Wastes", which clearly stated that by 2020, the comprehensive utilization rate of livestock and poultry manure in the country will reach more than 75%, which shows that it is imperative to implement the resource utilization of livestock and poultry manure. In line with the principle of "reduction, harmlessness, and resource utilization", the resource utilization of livestock and poultry manure can not only turn waste int...

Claims

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Application Information

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IPC IPC(8): C05F3/00C05F17/50A01K67/033
CPCC05F3/00C05F17/50A01K67/033Y02W30/40
Inventor 胡文锋梅瀚杰李雪玲庞旭李楚君徐键
Owner SOUTH CHINA AGRI UNIV
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