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Method for efficiently removing antibiotic residues in livestock manure through normal-temperature fermentation

A technology of livestock and poultry manure, normal temperature fermentation, applied in chemical instruments and methods, biological sludge treatment, animal husbandry wastewater treatment and other directions, can solve problems such as inability to withstand high temperature, and achieve enhanced degradation, high removal efficiency, and simple temperature control. Effect

Active Publication Date: 2016-04-20
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, many antibiotic-degrading bacteria screened are normal temperature microorganisms and cannot tolerate high temperature

Method used

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  • Method for efficiently removing antibiotic residues in livestock manure through normal-temperature fermentation

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Experimental program
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Effect test

Embodiment 1

[0025] Test material: pig manure; the type is free-range pigs raised by farmers; the appearance is dark brown, relatively wet, and the smell is very strong; the original pH is 7.4; 5 kinds of sulfonamides, 5 kinds of quinolones, 3 Four kinds of tetracyclines and three kinds of macrolide antibiotics, of which the total residual concentration of tetracyclines per kilogram of dry pig manure is 77.4mg, the total residual concentration of quinolones is 35.9mg, the total residual concentration of sulfonamides is 15.1mg and macrolides The total residual concentration is 12.8mg.

[0026] A method for efficiently removing antibiotics in livestock and poultry manure by normal temperature fermentation, the method consists of the following steps:

[0027] (1) Add concentrated sulfuric acid with a mass ratio of 1% to 500g of fresh pig manure, stir well, and place it at room temperature around 25°C for 1-2 hours;

[0028] (2) Use 10M sodium hydroxide solution to adjust the pH of the pig ma...

Embodiment 2

[0035] Test material is the same as embodiment 1.

[0036] A method for efficiently removing antibiotics in livestock and poultry manure by normal temperature fermentation, the method consists of the following steps:

[0037] (1) Add concentrated sulfuric acid with a mass ratio of 1% to 500g of fresh pig manure, stir well, and place it at room temperature around 25°C for 1-2 hours;

[0038] (2) Using 10M sodium hydroxide solution to adjust the pH of the pig manure treated in step (1) to 5.4;

[0039] (3) Spread the livestock and poultry feces treated in step (2) on an 18cm petri dish, and place them in a light incubator with 24 hours of light and 0 hours of darkness. The light source is LED energy-saving light source, and cultivated at 30°C for 4 -5 days.

[0040] In this example, the control treatment is to spread the livestock and poultry feces treated in step (2) on an 18cm petri dish, use newspapers for shading culture, place them in a light incubator, and cultivate them...

Embodiment 3

[0042] Test material is the same as embodiment 1.

[0043] A method for efficiently removing antibiotics in livestock and poultry manure by normal temperature fermentation, the method consists of the following steps:

[0044](1) According to the weight percentage of 10% and 20%, wood chips are mixed with fresh pig manure;

[0045] (2) Add concentrated sulfuric acid with a mass ratio of 3% to the pig manure sawdust mixture treated in step (1), stir evenly, and place it at room temperature around 25°C for 1-2 hours;

[0046] (3) Use 10N sodium hydroxide solution to adjust the pH of the sample treated in step (2) to 5.4;

[0047] (4) Spread the sample treated in step (3) on an 18cm petri dish and culture it at 30°C for 4-5 days.

[0048] In this example, after the fermented pig manure samples were freeze-dried, the antibiotic content was determined by high performance liquid chromatography-tandem mass spectrometry. In the present embodiment, after 4-5 days of solid-state ferme...

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Abstract

The invention provides a method for efficiently removing antibiotic residues in livestock manure through normal-temperature fermentation. The method includes the following steps that 1, concentrated sulfuric acid with the mass ratio of 1%-3% is added into the livestock manure, and acid regulating treatment is carried out for 1-2 hours; 2, pH is adjusted to range from 4.5 to 6.0; 3, the treated manure is spread to a sealed or semi-sealed container, the temperature is controlled to range from 28 DEG C to 35 DEG C, and solid state fermentation is carried out for 4-6 days. A light source is arranged in a fermenting container for illumination cultivation, and wood shavings are added into the livestock manure, so that degradation of antibiotics is accelerated. The antibiotic pollutants can be any one of tetracycline antibiotics, sulfonamide antibiotics and fluoroquinolone antibiotics or a mixture of several of tetracycline antibiotics, sulfonamide antibiotics and fluoroquinolone antibiotics, and the method is particularly suitable for removing tetracycline antibiotics and sulfonamide antibiotics. Compared with an existing technology for removing antibiotics in the livestock manure, it can be achieved that the content of the antibiotics in the livestock manure is reduced in a short time under the normal temperature condition, and the method does not depend on the high temperature condition.

Description

technical field [0001] The invention belongs to the technical field of environmental protection, and in particular relates to a method for efficiently removing residual antibiotics in livestock and poultry feces by normal temperature fermentation. Background technique [0002] The contamination of antibiotics in manure from livestock and poultry farming has aroused great concern. According to reports, about 6,000 tons of veterinary antibiotics are used in livestock and poultry feed additives in my country every year, accounting for 50% of the global antibiotic feed additive usage. The use of these antibiotics can promote the growth of livestock and poultry, prevent the occurrence of diseases, and promote the rapid development of the breeding industry to a large extent. The poultry manure is excreted and eventually enters the environment through fertilization and other methods. Previous studies have shown that vegetable crops such as cucumbers and lettuces have significant ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F11/00C02F11/02C02F103/20
CPCC02F11/00C02F11/02C02F2103/20
Inventor 林辉马军伟孙万春符建荣叶静俞巧钢王强邹平姜丽娜
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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