A strain of low-temperature-resistant multifunctional bacteria and its application in soil and water pollution remediation

The TLT1 strain of the genus Bacillaceae obtained through gradient domestication stabilizes zinc and degrades enrofloxacin at low temperatures, solving the problem of heavy metal and antibiotic pollution in soil and water bodies in cold northern regions and achieving efficient and environmentally friendly remediation effects.

CN117551557BActive Publication Date: 2025-10-17SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI +1
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Patent Information

Application Number
CN202311453800.4
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2023-11-03
Publication Date
2025-10-17
Estimated Expiration
2043-11-03

AI Technical Summary

Technical Problem

Existing technologies make it difficult to effectively stabilize heavy metal zinc and degrade the antibiotic enrofloxacin under different temperature conditions, especially in cold northern regions where livestock and poultry manure contaminates soil and water, causing pollutants to threaten human health through the food chain.

Method used

Provided is a Talcomyces TLT1 strain, which is a low-temperature-resistant strain obtained through gradient domestication. The strain TLT1 can stabilize zinc and degrade enrofloxacin in a low-temperature environment, and can be made into a bacterial agent for soil and water remediation.

Benefits of technology

Under low and normal temperature conditions, strain TLT1 significantly degrades enrofloxacin and stabilized zinc with high degradation efficiency, low cost, and no secondary pollution, making it suitable for the remediation of complex-contaminated soil and water bodies.

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Abstract

The present invention belongs to the technical field of biological treatment of environmental pollutants, and specifically relates to a strain of low-temperature-resistant multifunctional bacteria and its application in soil and water pollution remediation. The low-temperature-resistant multifunctional bacteria is the strain TLT1 of the genus Tularemia, which was deposited in the General Microbiology Center of the China Microorganism Culture Collection Administration on August 9, 2023, with the deposit number: CGMCC No: 40754. The bacterial agent of the present invention can be used to stabilize zinc in the soil environment, which helps to enrich the database of bacterial strains that stabilize heavy metals and have the ability to degrade quinolone antibiotics. The present invention prepares the purified strain into a bacterial suspension, which can effectively stabilize zinc in different environmental media such as water and soil under low-temperature conditions, and has the ability to degrade enrofloxacin in different environmental media such as water and soil. Compared with methods such as chemical oxidation, this method is more efficient, less expensive, and has no secondary pollution, providing an effective biological-non-biological combined remediation technology for the remediation of zinc-enrofloxacin complex-contaminated soil and water.
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