New application of quinolone compounds in prevention and treatment of plant bacterial diseases such as citrus canker

A technology of citrus canker and citrus canker, applied in plant growth regulators, botanical equipment and methods, applications, etc., can solve the problems of quinolone compounds that have not yet been reported, and achieve excellent antibacterial activity

Pending Publication Date: 2020-10-16
LANZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The prior art discloses that oxolinic acid (CN 201910307325), ciprofloxacin (CN 201510069315), levofloxacin (CN201810599892) and nafloxacin (CN201811479010) have good control effects on bacte

Method used

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  • New application of quinolone compounds in prevention and treatment of plant bacterial diseases such as citrus canker
  • New application of quinolone compounds in prevention and treatment of plant bacterial diseases such as citrus canker

Examples

Experimental program
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Effect test

Embodiment

[0010] Embodiment: Indoor antibacterial activity assay and result

[0011] Micro-broth dilution method was used: X. citri was inoculated in 20 mL of MH medium, and then placed in a constant temperature shaker at 37° C. for 12 hours at a rotational speed of 150 rpm. Take an appropriate amount of bacterial culture solution and use a microplate reader to measure the absorbance at 600nm, and use the culture solution without bacteria as a blank control. 600 0.1 is equivalent to a concentration of 1×10 8 cfu / mL, the bacterial suspension was diluted to a concentration of 1×10 with MH medium 6 cfu / mL bacterial solution, set aside.

[0012] Use a row gun to draw 50 μL of the diluted bacterial solution into a 96-well plate, and then add 50 μL of the compound solution in sequence. The blank control is to add the same volume of PBS solution, and each concentration is set for 3 replicates. After the sample was incubated in a constant temperature incubator at 28°C for 24 hours, its OD va...

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Abstract

The invention discloses a new application of quinolone compounds as bactericides in prevention and treatment of bacterial diseases and citrus canker of crops. The quinolone compounds comprise floroxacin, enofloxacin, gatifloxacin, moxifloxacin hydrochloride, enrofloxacin, marbofloxacin, floxacin, mononorfloxacin mesylate, prulifloxacin, Balofloxacin, pazufloxacin mesylate, pipemidic acid, sparfloxacin, difloxacin hydrochloride, lomefloxacin hydrochloride, pefloxacin, tosufloxacin mesylate, Cinoxacin, galafloxacin, besifloxacin hydrochloride, ofloxacin, nalidixic acid, Clinafloxacin and Sitafloxacin. The quinolone compounds can be used for preventing and treating bacterial diseases caused by citrus canker pathogens, especially gatifloxacin, moxifloxacin hydrochloride, mononorfloxacin mesylate, sparfloxacin, tosufloxacin mesylate, clinafloxacin and sitafloxacin, has excellent bacteriostatic activity on citrus canker pathogens, and can be used for preventing and treating bacterial diseases of crops.

Description

technical field [0001] The invention relates to the field of agricultural fungicides, in particular to the new application of quinolone compounds as fungicides in preventing and treating plant bacterial disease citrus canker. Background technique [0002] In recent years, bacterial diseases have become the second most common diseases in my country's agricultural production after fungal diseases. Plant bacterial diseases and the induced complications seriously threaten the healthy development of agriculture and food security, and have become one of the biggest problems in agriculture that should be solved immediately. However, at present, the types and quantities of pesticides for the prevention and treatment of bacterial diseases in my country are limited, and there are few new varieties. The long-term use of these medicaments has led to the increasingly serious drug resistance of germs and the decline in the control effect of existing medicaments. Therefore, the research ...

Claims

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Application Information

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IPC IPC(8): A01N43/90A01N43/42A01N43/46A01N43/60A01P1/00
CPCA01N43/42A01N43/46A01N43/60A01N43/90
Inventor 刘映前张智军贺颖慧赵文斌吴天琳杜莎莎杨程杰
Owner LANZHOU UNIVERSITY
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