Ureaplasma urealyticum/mycoplasma hominis combined rapid culture and drug sensitivity detection kit

Abstract

Provided is a ureaplasma urealyticum/mycoplasma hominis combined rapid culture and drug sensitivity detection kit. The kit can complete detection in 8-24 h, and meanwhile, drug-resistant detection of various antibiotics can be performed. The combined rapid culture kit is mainly composed of pork stomach digestive juice or lung digestive juice, a beef extracting solution or a beef heart extracting solution, cattle serum or horse serum or other animal serum, sodium chloride, peptone, yeast powder, urea, L-arginine, phenol red indicator or cresol red indicator, and penicillin or other antibiotics. The drug detection kit can detect doxycycline hyclate, roxithromycin, acetylspiramycin, gatifloxacin, clarithromycin, azithromycin, clindamycin, erythromycin, kitasamycin, ofloxacin, levofloxacin hydrochloride, sparfloxacin, ciprofloxacin, doxycycline, erythromycin cydocarbonate and various other antibiotics. By means of the detection kit, not only is the mycoplasma detection time shortened, but also drug-resistant detection can be performed at the same time so that clinical medication can be guided. The detection kit has the beneficial effects of being high in sensitivity, high in specificity, high in detection rate, easy and convenient to operate and the like.

Description

technical field [0001] The invention belongs to the field of medicine, and relates to biological diagnostic reagents, in particular to a combined rapid culture and drug sensitivity detection kit of Ureaplasma urealyticum / Mycoplasma hominis. , has the advantages of high sensitivity, strong specificity, high detection rate, and easy operation, and can be widely used in clinical testing in various hospitals. Background technique [0002] The incidence rate of non-gonococcal urogenital tract inflammation (NGU) reported in 2007 has surpassed that of gonorrhea, ranking first among the eight sexually transmitted diseases in the country. Chlamydia and mycoplasma are the main pathogens that cause NGU. Chlamydia is mainly Chlamydia trachomatis, and mycoplasma is mainly Ureaplasma urealyticum (UU) and Mycoplasma hominis (MH). Due to the limited diagnostic conditions and naming problems. There is no systematic population survey data in China. Mycoplasma can exist in healthy carriers, ...

AI Technical Summary

Problems solved by technology

Mycoplasma cells can be isolated and cultured to obtain mycoplasma, which can be confirmed, which is the "gold standard" of traditional laboratory diagnosis. However, due to the cumbersome operation of this method, it takes about one week to report the results, so it has not been widely used.

The detection of plasmid nucleic acid by PCR method is fast, s