Preparation method of renewable electrochemical immunosensor for sCD40L detection

An immunosensor, electrochemical technology, applied in the direction of electrochemical variables of materials, scientific instruments, instruments, etc., can solve the problems of uneven dispersion and easy agglomeration, and achieve the effects of firm bonding, enhanced conductivity, and fast detection speed.
CN106483281AInactive Publication Date: 2017-03-08CHONGQING MEDICAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
CHONGQING MEDICAL UNIVERSITY
Publication Date
2017-03-08
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a preparation method of a renewable electrochemical immunosensor capable of being used for detecting a biomarker for forecasting and diagnosing an acute coronary syndrome, i.e., a soluble CD40 ligand (sCD40L), and belongs to the technical field of electrochemical detection. The preparation method is characterized by comprising the following step: immobilizing an sCD40L antibody through a carboxyl functionalized multi-wall carbon nano tube-polyethyleneimine-gold nanoparticle nano compound (c-MWCNTs-PEI-AuNPs) serving as a substrate material to realize capturing of sCD40L and further quantitatively detecting the sCD40L. A c-MWCNTs-PEI-AuNps nano compound is easy to prepare, high in conductivity, relatively high in stability and relatively large in specific surface area, so that a large quantity of antibodies can be firmly immobilized; furthermore, by the use of specificity identification of the antibodies and antigens, the built electrochemical immunosensor is relatively high in specificity. The electrochemical immunosensor has the advantages of high sensitivity, high specificity, convenience, quickness and capability of being repetitively used for multiple times; a new method is supplied to detection of the sCD40L, and available information is supplied to clinical forecasting and diagnosis of the acute coronary syndrome.
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Description

[0001] Technical field:

[0002] The present invention relates to a method for the preparation of an electrochemical immunosensor for the detection of soluble CD40 ligand (sCD40L), which can be used as a good and reliable biochemical marker for prediction and diagnosis of acute coronary syndrome, based on carboxyl-functionalized multi-walled carbon Direct regenerative immunosensors of nanotubes (c-MWCNTs), polyethyleneimine (PEI), and gold nanoparticles (AuNPs) nanocomposites for the detection of sCD40L belong to the field of electrochemical detection.

[0003] Background technique:

[0004] At present, cardiovascular disease has become a major cause of human death due to its high morbidity, disability and mortality, threatening human health, and acute coronary syndrome is a common and serious cardiovascular disease in clinical practice. . sCD40L is a member of the tumor necrosis factor superfamily and belongs to type I transmembrane glycoprotein, which can promote cell expans...

Claims

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