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Magnetic immunity detection method

An immunodetection method and magnetic technology, which can be used in measurement devices, instruments, scientific instruments, etc., can solve the problems of complex and bulky microplate readers, cumbersome detection steps, and expensive enzymes, and achieve portability, detection sensitivity and accuracy. The effect of improving and speeding up detection

Inactive Publication Date: 2010-05-12
UNIV OF SCI & TECH BEIJING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to propose a brand-new magnetic immunoassay method for the defects of the above enzyme-linked immunoassay method such as low sensitivity, cumbersome detection steps, expensive enzyme price, complex and bulky microplate reader, etc.

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0051] The hepatitis C antibody is coupled to the surface aminated magnetic methyl methacrylate microsphere in advance, and the hepatitis C antibody is also coupled to the inner surface of the microwell plate. Add 50uL serum samples containing hepatitis C antigen at a certain concentration into the microwell plate, react at 37°C for 5 minutes, wash 5 times with 0.02MPBST (Tween20, 0.5%), and wash away the hepatitis C antigen that does not react with the hepatitis C antibody on the microwell plate. Add 20 uL of magnetic methyl methacrylate microsphere solution (concentration: 35mg / mL) coupled with hepatitis C antibody, react at 37°C for 5min, wash 5 times with 0.02MPBST (Tween20, 0.5%), and wash away the microwell plate bound magnetic microspheres. Extend the magnetic head of the giant magnetoresistance biosensor developed by Sun Yat-sen University into the microwell plate, and determine the presence and concentration of magnetic microspheres on the surface of the microwell pla...

Embodiment 2

[0053]The hepatitis A antibody is coupled to the surface aminated magnetic methyl acrylate microsphere in advance, and the hepatitis A antibody is also coupled to the magnetoresistance film surface of the tunnel magnetoresistance sensor. Take 3 drops (about 0.9uL) of a certain concentration of serum samples containing hepatitis A antigen with a micro-sampler and drop them on the surface of the sensor magnetoresistance film, react at 37°C for 5 minutes, wash 5 times with 0.02MPBST (Tween20, 0.5%), wash Remove the hepatitis A antigen that has not reacted with the hepatitis A antibody on the membrane surface. Then take 3 drops (about 0.9uL) of magnetic methyl acrylate microsphere solution (concentration: 30mg / mL) with a micro-sampler, add it dropwise on the surface of the magnetic resistance film of the sensor, react at 37°C for 5min, and use 0.02MPBST (Tween20, 0.5%) was washed 5 times to wash away the magnetic microspheres not coupled to the hepatitis A antibody on the membrane...

Embodiment 3

[0055] Hepatitis B antibody was coupled to the surface aminated magnetic polystyrene microspheres in advance, and hepatitis B antibody was also coupled to the surface of the giant magnetoresistive biosensor magnetic head developed by Sun Yat-sen University. Add 50uL of a certain concentration of serum containing hepatitis B antigen to 20uL of magnetic polystyrene microsphere solution coupled with hepatitis B antibody (concentration: 40.5mg / mL), react at 37°C for 5min, and magnetically separate off the surface of the magnetic microsphere that is not connected to the surface of the magnetic microsphere. HBV antigen for HBV antibody response. The magnetic head of the magnetoresistive sensor was inserted into the magnetic microsphere solution, reacted at 37°C for 5 minutes, washed 5 times with 0.02MPBST (Tween20, 0.5%), and the magnetic microspheres not coupled to the hepatitis C antibody on the surface of the magnetic head were washed away. The existence and concentration of the ...

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Abstract

The invention relates to a magnetic immunity detection method for antigens (antibodies), which comprises the steps: (1) respectively coupling the antibodies (antigens) to the surfaces of magnetic polymer microspheres and a micro-pore plate; (2) instilling the detected antigens into the micro-pore plate; (3) cleaning the micro-pore plate and then adding the magnetic polymer microspheres in the micro-pore plate; and (4) cleaning the micro-pore plate once again and stretching the magnetic head of a magnetoresistive sensor into the pore of the micro-pore plate, and determining the number of the magnetic polymer microspheres on the surface of the micro-pore plate according to the variation between former output voltage and latter output voltage of the magnetic reluctance sensor, thus further determining the presence and the concentration of the virus antigens (antibodies). The method has the characteristics of few detection steps, high speed, high sensitivity and accuracy, low cost and the like.

Description

technical field [0001] The invention relates to an immunological detection method of an antigen (antibody), in particular to a magnetic immunological detection method for detecting the antigen (antibody) by using the principle of magnetoresistance. Background technique [0002] Immunological detection technology refers to the establishment of various detection and analysis technologies based on the principle of specificity of immune response. The immune response refers to various specific reactions that occur when antigens are combined with antibodies. Current immunoassay labeling technologies include enzymes, colloidal gold, fluorescence, and chemiluminescence. [0003] Enzyme immunoassay is an immunological technique developed in the early 1970s. The enzyme immunoassay method organically combines the high efficiency of the enzymatic reaction with the high specificity of the immune reaction to achieve the purpose of measuring many substances. Enzyme immunoassay is an imm...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/546G01N33/576
CPCY02A50/30
Inventor 赖仙红官月平谢立刘刚戈莹
Owner UNIV OF SCI & TECH BEIJING
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