Method for cultivating rice breeding material with broad spectrum and lasting spike blast resistance

A technology for resistance breeding and panicle blast, applied in the field of rice molecular breeding, to achieve the effects of reducing detection steps, improving breeding selection efficiency, and slowing down the mutation rate

Active Publication Date: 2015-10-14
JIANGSU LIXIAHE REGION AGRI RES INST
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

, but there is no research yet to report whether the aggregation of these two genes has the effect of panicle blast resistance

Method used

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  • Method for cultivating rice breeding material with broad spectrum and lasting spike blast resistance
  • Method for cultivating rice breeding material with broad spectrum and lasting spike blast resistance
  • Method for cultivating rice breeding material with broad spectrum and lasting spike blast resistance

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Experimental program
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Embodiment 1

[0026] (1) Gumei 4, the donor parent carrying the resistance gene Pigm (Yiwen Deng, et al. Genetic characterization and fine mapping of the blast resistance locus Pigm(t) tightly linked to Pi2 and Pi9 in a broad-spectrum resistant Chinese variety .Theoretical and Applied Genetics,2006,113(4):705-713) and the donor parent K3 carrying the resistance gene Pi54 (Yamada M, et al.Proposal of a new method for differentiating races of Pyricularia oryzae Cavara in Japan. Ann Phytopathol Soc Jpn,1976,42(2):216-219) were crossed with the recurrent parent Yangdao 6 respectively to obtain F 1 , respectively with F 1 - Pigm, F 1 -Pi54 said.

[0027] (2) planting F 1 -Pigm and F 1 -Pi54 seeds, the recurrent parent Yangdao No. 6 was backcrossed at flowering stage to obtain 1 generation of backcross, respectively with BC 1 f 1 -Pigm, BC 1 f 1 -Pi54 said.

[0028] (3) planting BC 1 f 1 -Pigm, BC 1 f 1 -Pi54 seeds, each BC was extracted at the seedling stage 1 f 1 Isolate the DNA ...

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Abstract

The invention discloses a method for cultivating a breeding material with broad spectrum and lasting spike blast resistance, belonging to the technical field of rice molecular breeding. The method is characterized in that with a parent of conventional rice or hybrid rice with large production extension area and excellent comprehensive characters as a recurrent parent, broad-spectrum anti-disease genes, Pigm and Pi54, are polymerized in the breeding material through continuous backcross and convergent cross and with combination of marker-assisted selection; meanwhile, representative strains are selected for performing artificial inoculation identification of spike blast, natural inducedidentification in rice blast serious areas and basic agronomic character evaluation during a total growth period, and an advanced line with excellent spike blast resistance is obtained. The agronomic characters of the advanced line bred through the method are similar to or consistent with those of the recurrent parent, and the broad spectrum and lasting resistance to spike blast is enhanced obviously.

Description

technical field [0001] The invention belongs to the technical field of rice molecular breeding, and relates to a method for cultivating broad-spectrum and long-lasting panicle blast-resistant rice breeding materials. Background technique [0002] Rice blast caused by the fungus Magnaporthe oryzae is one of the most serious diseases of rice, which seriously threatens the sustained high and stable yield of rice. According to statistics, the global annual output loss due to rice blast is 10%-30%, the direct economic loss is about 5 billion US dollars, and the lost grain is enough to feed 60 million people. In my country, rice blast occurs to varying degrees every year. Especially in recent years, it has continued to occur in the southwestern, middle and lower reaches of the Yangtze River, and Northeast. kg, which has brought huge hidden dangers to the safe production of rice in my country. Practice has proved that using disease-resistant genes to breed resistant varieties is o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/04
Inventor 李爱宏吴云雨戴正元余玲潘存红肖宁李育红张小祥周长海黄年生赵步洪季红娟刘晓静蒋敏
Owner JIANGSU LIXIAHE REGION AGRI RES INST
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