Method for preparing ionic rare earth mine tailing in-situ remediation plant nursing system
An ion-type rare earth and in-situ repair technology, applied in the biological field, can solve the problems of high cost and unfavorable large-scale application of plant growth-promoting bacteria agents, and achieve the effects of small environmental impact, improvement of soil physical and chemical conditions, and growth promotion
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[0020] 1) Activate the strain of Bacillus amyloliquefaciens H47 with the preservation number CCTCCNO: M2013283 and prepare it into a plant growth promoting agent:
[0021] (1) Take one ring of the slant bacterial species, streak the hyphae on the PDA plate, and cultivate it at 30° C. for 48 hours in an incubator;
[0022] (2) pick single bacterium colony on the PDA plate and insert the single bacterium colony on the plate into the 250ml Erlenmeyer flask that 50ml nutrient solution is housed, prepare 9 with rotating speed 200rpm on the shaker, the condition constant temperature culture of temperature 30 ℃ for 5 days, obtain Plant growth promoter.
[0023] 2) Biomass carrier pretreatment: Take 3 kg of rice husk, mix according to the mass ratio: 60% rice husk, 20% waste molasses, and 3% bean cake powder, add water to the water content of 40%, adjust the pH to 7.0, and install it in a high-temperature resistant Sterilize at 121°C for 30 minutes in a plastic bag.
[0024] 3) Add ...
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