Myclobutanil colloidal gold test strip and preparation method and application thereof

A technology for detecting test paper and myclobutanil, which is applied in the field of myclobutanil colloidal gold detection test strip and its preparation, can solve the problems of many interfering substances, being easily affected by impurity interference, long detection time, etc., and achieving accuracy and precision High degree of accuracy, easy to promote and use, and the effect of short detection time

Inactive Publication Date: 2019-02-01
YUNNAN ACAD OF TOBACCO AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, ultra-high performance liquid chromatography-tandem mass spectrometry and gas chromatography-tandem mass spectrometry detection are susceptible to interference from impurities, requiring complex sample pretreatment processes, a large amount of solvents used in the detection process are likely to pollute the environment, and the required detection time is long, which poses a threat to the detection personnel. High professional ability requirements
Due to the complexity of tobacco components, such as nicotine content can reach 1 to 3%, and the protein content in tobacco leaves is also very high, generally up to 15%, up to 17%, resulting in many interfering substances, the detection methods in the prior art cannot Effectively remove impurities such as nicotine and protein in tobacco leaves, but the existence of nicotine and protein is easy to form interference peaks, which affects the detection accuracy of the target object, so it is not fully suitable for the determination of tobacco

Method used

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  • Myclobutanil colloidal gold test strip and preparation method and application thereof
  • Myclobutanil colloidal gold test strip and preparation method and application thereof
  • Myclobutanil colloidal gold test strip and preparation method and application thereof

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preparation example Construction

[0026] The preparation method of the myclobutanazole colloidal gold detection test strip of the present invention comprises the preparation of myclobutanazole antigen, the preparation of myclobutanazole polyclonal antibody, the preparation of colloidal gold, the preparation of myclobutanazole polyclonal antibody-colloidal gold marker, the preparation of sheep antibody Rabbit anti-antibody preparation, colloidal gold detection test strip assembly steps, specifically include:

[0027] A. Myclobutanil antigen preparation:

[0028] The synthesis route of myclobutanazole antigen is as follows:

[0029]

[0030] A. Myclobutanil antigen preparation:

[0031] A1. Weigh 1.45 g of myclobutanil and dissolve it in 2 mL of dichloromethane, then add 2.5 mL of 30wt% hydrogen peroxide aqueous solution, 0.34 g of tetrabutylammonium bisulfate and 1.9 mL of 20wt% sodium hydroxide aqueous solution, and react The mixture was stirred at room temperature at 1000-2000 rpm for 24 h, then diluted ...

Embodiment

[0055] Example: Preparation of Myclobutanil Colloidal Gold Test Strips

[0056] 1. Preparation of myclobutanazole antigen, that is, the preparation of myclobutanazole immune antigen and myclobutanazole-coated antigen:

[0057] 1.1. Weigh 1.45 g of myclobutanil and dissolve it in 2 mL of dichloromethane, then add 2.5 mL of 30wt% hydrogen peroxide aqueous solution, 0.34 g of tetrabutylammonium bisulfate and 1.9 mL of 20wt% sodium hydroxide aqueous solution, and react The mixture was stirred at room temperature at 1000-2000 rpm for 24 h, then diluted with 5 mL of dichloromethane, separated by TLC, washed with 5×2 mL of brine, and the precipitated white solid I was collected.

[0058] 1.2. Dissolve 0.7 g of white solid I in 0.96 mL of concentrated sulfuric acid, and heat to 70 °C, then add 0.3 mL of 0.7 g / mL sodium nitrite solution, continue to heat up to 105 °C and stir for 3 h, then cool to At room temperature, add 0.5mL of 0.01moL / L sodium hydroxide solution, extract 3 times w...

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Abstract

The invention discloses a myclobutanil colloidal gold test strip and a preparation method and application thereof. A sample absorbing mat, a conjugate releasing mat and a reaction film of the test strip are sequentially combined on one face of a PVC bottom plate from left to right and from top to bottom. The conjugate releasing mat is overlapped with one end of the reaction film, and the water absorbing mat is combined at the other end of the reaction film. The reaction film covers a detecting line composed of myclobutanil antigens and a quality control line composed of second antibodies, andthe conjugate releasing mat covers a myclobutanil antibody-colloidal gold marker. The preparation method includes the steps of myclobutanil antigen preparation, myclobutanil multi-clonal antibody preparation, colloidal gold preparation, myclobutanil multi-clonal antibody-colloidal gold marker preparation, goat anti-rabbit antibody preparation and colloidal gold test strip assembling. The myclobutanil colloidal gold test strip is applied to detect myclobutanil in plant tissues. The myclobutanil colloidal gold test strip is simple, rapid and accurate in detection, can simultaneously detect a large batch of samples, and meets the requirement for rapid detection of myclobutanil residues in plant tissues such as tobaccos.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and in particular relates to a myclobutanil colloidal gold detection test strip and a preparation method and application thereof. Background technique [0002] Myclobutanil, common name: Myclobutanil, is a class of systemic triazole fungicides with protective and therapeutic activity. Rohm and Haas developed myclobutanazole in 1988 and used it for the fungicidal control of a series of crops. Myclobutanazole mainly inhibits the biosynthesis of ergosterol of pathogenic bacteria, and has good control effects on Ascomycetes and Basidiomycetes. It has the characteristics of strong systemic absorption, high efficacy, and long-lasting effect. Disease, rust, scab, gray spot, brown spot, smut. my country is a big tobacco country. With the continuous increase of tobacco varieties and quantities, the occurrence of tobacco diseases and insect pests is also increasing. Myclobutanil is widely us...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/94G01N33/558G01N33/531G01N33/532
CPCG01N33/531G01N33/532G01N33/558G01N33/9446
Inventor 逄涛李勇师君丽晋艳卢秀萍罗贵昆孔光辉
Owner YUNNAN ACAD OF TOBACCO AGRI SCI
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