Chlorpyrifos detecting method and device by terahertz wave spectrum combining with biosensing technique

A biosensing and detection method technology, applied in the field of chlorpyrifos detection, can solve problems such as slow speed, difficulty in adapting to rapid detection requirements, complicated operation, etc., and achieve high detection efficiency, high accuracy, and time-saving effects

Active Publication Date: 2014-02-05
ZHEJIANG UNIV
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Problems solved by technology

However, ELISA needs to fix the antibody for the second time after fixing the antigen. The operation is relatively complicated and the speed is slow, and it is difficult to adapt to the growing demand for rapid detection.

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  • Chlorpyrifos detecting method and device by terahertz wave spectrum combining with biosensing technique
  • Chlorpyrifos detecting method and device by terahertz wave spectrum combining with biosensing technique

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Embodiment Construction

[0029] Terahertz waves are generally considered to be electromagnetic waves with a frequency of 0.1-10 THz, and their wavelengths are between microwaves and infrared radiation. Terahertz waves have very low energy and will not cause ionization reactions that are harmful to the human body or detection materials. In general, the vibrational absorption frequency of chemical bonds in organic molecules is mainly manifested in the ordinary infrared band. However, for relatively weak interactions between molecules (such as hydrogen bonds, etc.) Hertz infrared band. Therefore, although the energy of terahertz radiation is very low, a large number of molecules, especially many organic macromolecules (DNA, protein, etc.), show strong absorption and dispersion in this frequency band. Terahertz time-domain spectroscopy (THz-TDS) is a new research and detection technology developed internationally in recent years. So far, terahertz time-domain spectroscopy technology has many applicatio...

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Abstract

The invention relates to a chlorpyrifos detecting method and device by a terahertz wave spectrum combining with a biosensing technique, which have the characteristics of high accuracy and quickness and convenience in detection. According to the technical scheme, the chlorpyrifos detecting method comprises the following steps: (1) cleaning quartz slides; (2) modifying the surfaces of the quartz slides; (3) activating the surfaces of the quartz slides; (4) fixing an antibody; (5) sealing excessive position points on the surfaces of the quartz slides; (6) fixing chlorpyrifos; (7) collecting the terahertz time domain wave spectrums of to-be-detected points and reference points on the quartz slides; and (8) comparing the terahertz time domain wave spectrums of the to-be-detected points and the reference points on the quartz slides. The chlorpyrifos detecting device comprises a terahertz time domain wave spectrum system, a motor and the quartz slides.

Description

technical field [0001] The invention relates to a chlorpyrifos detection method and device, in particular to a chlorpyrifos detection method and device using terahertz spectrum combined with biosensing technology. Background technique [0002] Chlorpyrifos (Chlorpyrifos) is a moderately toxic organophosphorus insecticide, which has contact killing, stomach poisoning and fumigation effects, and can better control aboveground and underground pests of various crops. As one of the ideal varieties to replace highly toxic organophosphate insecticides, chlorpyrifos is widely used in agricultural production practices in my country. Although chlorpyrifos is not very toxic to humans, it is extremely toxic to aquatic organisms and may have long-term adverse effects on the water environment, so it is necessary to test for chlorpyrifos. The traditional chlorpyrifos detection method—enzyme-linked immunoassay (ELISA) is a pesticide residue detection method based on the specific and revers...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/3577
Inventor 应义斌徐文道谢丽娟刘湘江
Owner ZHEJIANG UNIV
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