Method for performing immunological test on biomolecules by avidin/streptavidin magnetic composite particles
A technology of composite particles and biomolecules, applied in the field of immunoassays, which can solve the problems of less labeling and inactivation of biomolecules
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Embodiment 1
[0051] (1) Coupling of biotinylated anti-HBsAg antibody to avidin / streptavidin magnetic composite particles
[0052] Dissolve 200 μl biotinylated anti-HBsAg antibody in 400 μl pH=7.4 Tris-HCl coupling buffer, mix well and add to avidin / streptavidin magnetic composite particles, at 37°C, 180rpm, in a shaker React for 20 minutes, remove the magnetic separation after the reaction is complete, discard the supernatant; add 400 μl 1×PBS, magnetically separate, discard the supernatant;
[0053] (2) closed
[0054] Add 1ml of Tris-HCl buffer containing 5% skimmed milk powder and free biotin to the avidin / streptavidin magnetic composite particles, react at 37°C, 180rpm, in a shaker for 1 hour, magnetically separate, and discard the supernatant . Wash 3 times with 2ml pH=7.4, 1×PBS washing buffer, suspend the magnetic particles in 1ml pH=7.4, 0.02M Tris-HCl storage buffer, and store at 4°C for later use.
[0055] See figure 1 shown.
[0056] (3) Combined with the analyte
[0057] ...
Embodiment 2
[0074] (1) Coupling of biotinylated HCV antigen to avidin / streptavidin magnetic composite particles
[0075] Dissolve biotinylated HCV antigen in 400 μl pH=7.4 acetate coupling buffer, mix well, add to avidin / streptavidin magnetic composite particles, react at 37°C, 180rpm, in a shaker for 20min, After the reaction, remove the magnetic separation, discard the supernatant; add 400 μl 1×PBS, magnetic separation, discard the supernatant;
[0076] (2) closed
[0077] Add 1ml of Tris-HCl buffer containing 5% skimmed milk powder and free biotin to the avidin / streptavidin magnetic composite particles, react at 37°C, 180rpm, in a shaker for 1 hour, magnetically separate, and discard the supernatant . Wash 3 times with 2ml pH=7.4, 1×PBS washing buffer, suspend the magnetic particles in 1ml pH=7.4, 1×PBS storage buffer, and store at 4°C for later use.
[0078] (3) Combined with the analyte
[0079] The above-prepared avidin / streptavidin magnetic composite particles coupled with biot...
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