Combined detection test paper of influenza A virus antigen and influenza B virus antigen and preparation method thereof

A technology of influenza A virus and influenza B virus, which is applied in the field of medical testing, can solve the problems of inability to detect influenza A and B influenza viruses at the same time, the high cost of influenza virus detection, and the low sensitivity of using nano-gold to reduce the number of people. The effect of concentrating the risk of infection, saving disposable consumables, and reducing the harm caused by diseases
CN102445537AInactive Publication Date: 2012-05-09GUANGZHOU WONDFO BIOTECH
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
GUANGZHOU WONDFO BIOTECH
Publication Date
2012-05-09
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses combined detection test paper of influenza A virus antigen and influenza B virus antigen and a preparation method thereof. The test paper comprises a sample pad, a fiberglass membrane containing a colloidal gold particle label, a nitrocellulose membrane and water absorbing paper, wherein the nitrocellulose membrane comprises a detection area which is coated with an influenza A virus antibody, a detection area which is coated with an influenza B virus antibody and a control area which is coated with an goat anti-rabbit antibody; the colloidal gold particle label comprises a micro signal amplification system and a colloidal gold labeled rabbit IgG antibody; and the micro signal amplification system is a colloidal gold particle-avidin-biotin-influenza A / B virus antibody. According to the invention, an avidin-biotin microsignal amplification system is added in a double-antibody sandwich detection system, the signal of a target antibody is enlarged, the detection sensitivity is increased, false negative or detection omission due to weak signals can be avoided, simultaneously combined detection can be carried out on the influenza A and B virus antigens, and the detection time, sample and cost can be saved.
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Description

technical field

[0001] The invention belongs to the field of medical testing, and in particular relates to a test paper for combined detection of influenza A virus antigen and influenza B virus antigen and a preparation method thereof. Background technique

[0002] Influenza, commonly called "flu", is an acute respiratory infectious disease caused by influenza virus. It is highly contagious and spreads mainly through coughing and sneezing. It usually breaks out in spring and winter. It is divided into type A (type A) influenza virus, type B (type B) influenza virus and type C (type C) influenza virus. Influenza A virus has a strong variability, followed by B virus, and C virus is very stable, so the detection of A virus and B virus is particularly important.

[0003] According to statistics, there are 600-1200 million influenza cases in the world every year, of which 3-5 million cases are severe influenza, 250-500 thousand people die, and the case fatality rate of severe in...

Claims

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