Combined detection test paper of influenza A virus antigen and influenza B virus antigen and preparation method thereof

A technology of influenza A virus and influenza B virus, which is applied in the field of medical testing, can solve the problems of inability to detect influenza A and B influenza viruses at the same time, the high cost of influenza virus detection, and the low sensitivity of using nano-gold to reduce the number of people. The effect of concentrating the risk of infection, saving disposable consumables, and reducing the harm caused by diseases

Inactive Publication Date: 2012-05-09
GUANGZHOU WONDFO BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The purpose of the present invention is to solve the defects in the prior art that the detection cost of influenza virus is high, the detection period is long, the sensitivity of using nano gold is slightly

Method used

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  • Combined detection test paper of influenza A virus antigen and influenza B virus antigen and preparation method thereof
  • Combined detection test paper of influenza A virus antigen and influenza B virus antigen and preparation method thereof
  • Combined detection test paper of influenza A virus antigen and influenza B virus antigen and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1 Joint inspection test paper of influenza A virus antigen and influenza B virus antigen of the present invention

[0039] Such as figure 1 Shown is a test strip for joint detection of influenza A virus antigen and influenza B virus antigen according to the present invention. Comprising a sample pad 1, a glass fiber membrane 2 tightly connected to one end of the sample pad 1 containing colloidal gold particle markers, a nitrocellulose membrane 3 closely connected to the other end of the glass fiber membrane 2, and the fiber The other end of the plain film 3 is closely connected to the absorbent paper 4, the sample pad 1, the glass fiber membrane 2, the nitrocellulose membrane 3 and the absorbent paper 4 are all arranged on the bottom plate 5, and the nitrocellulose membrane 3 includes a coated There is a detection zone 6 of influenza A virus antibody, a detection zone 7 coated with influenza B virus antibody and a control zone 8 coated with goat anti-rabbit ...

Embodiment 2

[0041] The preparation method of the test paper of embodiment 2 embodiment 1

[0042] Colloidal gold particle labeling is used in the present invention. Among them, the particle diameter of colloidal gold particles is nanometer (30-50nm). The adsorption mechanism of colloidal particles is to use its negatively charged properties under alkaline conditions to combine with positively charged groups of protein molecules by electrostatic attraction. Immunodiagnostic reagents. The preparation steps of the joint inspection test paper of influenza A virus antigen and influenza B virus antigen are as follows:

[0043] 1. Preparation of Influenza A / B Antibody

[0044] Use conventional methods to immunize mice with recombinant influenza A / B antigens or inactivated influenza antigens. After the mice immunized with influenza A / B antigens are fused with myeloma cells, they are identified by ELISA. The identified hybridomas are in Culture in ascites, screen positive clones, and isolate an...

Embodiment 3

[0055] Embodiment 3 Utilize the test paper in embodiment 1 to detect the method for type A / B influenza virus antigen in secretion fluid

[0056] In this example, the test paper of Example 1 is used to detect the influenza A / B virus antigen in the secretion fluid sample.

[0057] Include the following steps:

[0058] (1) Sample collection: Samples were collected using polyester sponge swabs with sterile PP (polypropylene) rods.

[0059] Nasal secretion collection method: When collecting nasal secretions, insert the swab into the place with the most secretions in the nasal cavity, gently rotate and push the swab toward the inside of the nasal cavity until the turbinate (about 2.0cm to 2.5cm from the nostril) is blocked, and stick it Rotate the swab three times on the nasal wall and remove the swab;

[0060] Throat secretion collection: Insert the swab from the mouth completely into the throat, and center on the throat wall and the reddened part of the palate tonsil, wipe the b...

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Abstract

The invention discloses combined detection test paper of influenza A virus antigen and influenza B virus antigen and a preparation method thereof. The test paper comprises a sample pad, a fiberglass membrane containing a colloidal gold particle label, a nitrocellulose membrane and water absorbing paper, wherein the nitrocellulose membrane comprises a detection area which is coated with an influenza A virus antibody, a detection area which is coated with an influenza B virus antibody and a control area which is coated with an goat anti-rabbit antibody; the colloidal gold particle label comprises a micro signal amplification system and a colloidal gold labeled rabbit IgG antibody; and the micro signal amplification system is a colloidal gold particle-avidin-biotin-influenza A/B virus antibody. According to the invention, an avidin-biotin microsignal amplification system is added in a double-antibody sandwich detection system, the signal of a target antibody is enlarged, the detection sensitivity is increased, false negative or detection omission due to weak signals can be avoided, simultaneously combined detection can be carried out on the influenza A and B virus antigens, and the detection time, sample and cost can be saved.

Description

technical field [0001] The invention belongs to the field of medical testing, and in particular relates to a test paper for combined detection of influenza A virus antigen and influenza B virus antigen and a preparation method thereof. Background technique [0002] Influenza, commonly called "flu", is an acute respiratory infectious disease caused by influenza virus. It is highly contagious and spreads mainly through coughing and sneezing. It usually breaks out in spring and winter. It is divided into type A (type A) influenza virus, type B (type B) influenza virus and type C (type C) influenza virus. Influenza A virus has a strong variability, followed by B virus, and C virus is very stable, so the detection of A virus and B virus is particularly important. [0003] According to statistics, there are 600-1200 million influenza cases in the world every year, of which 3-5 million cases are severe influenza, 250-500 thousand people die, and the case fatality rate of severe in...

Claims

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Application Information

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IPC IPC(8): G01N33/569
Inventor 王继华张健郭诗静
Owner GUANGZHOU WONDFO BIOTECH
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