30results about "Magnetic immunoreagent carriers" patented technology

Compositions, methods, and systems for analyzing the protein corona are described herein, as well as its application in the discovery of advanced diagnostic tools as well as therapeutic targets.

Disclosed are systems, methods, and computer software for determining a conformational change in a structure of a protein. One method includes delivering a magnetic nanoparticle-nanobody (MNP-NB) complex to a sample containing a protein, where the MNP-NB complex will bind to the protein in the sample. An external magnetic field is applied to the sample with a magnetic field generation system. Signals are detected from the MNP-NB complex that reflect a response to the external magnetic field and a conformational change in a structure of the protein in the sample is determined from the signals.

The present invention discloses a magnetic quantum dot-encoded microsphere, a preparation method thereof, and a device for high-throughput detection. The preparation method includes the following steps: S1. Prepare a continuous phase solution and a dispersed phase solution; the dispersed phase includes a dispersed phase doped with quantum dot particles and a dispersed phase doped with magnetic nanoparticles; S2. Prepare uniform droplets through a microfluidic chip; push the continuous phase and the dispersed phase at a set speed, and the dispersed phase doped with quantum dot particles and the dispersed phase doped with magnetic nanoparticles always maintain laminar flow. After the dispersed phase is sheared into droplets, an obvious demarcation line in the middle of the droplets can be seen; S3. Add a photoinitiator so that the droplets can be rapidly cured into microspheres with a Janus structure after being irradiated by ultraviolet light in the channel; S4. Introduce functional groups on the surface of the microspheres for specifically binding to the antigen to be detected. The microspheres prepared by the method in the present invention can be covalently coupled with specific antibodies, used for specifically binding to the antigen to be detected, and can encapsulate more magnetic materials.

To provide compositions, methods, and systems for protein corona analysis and their uses.SOLUTION: Compositions, methods, and systems for analyzing protein corona are described, as well as their application in the discovery of advanced diagnostic tools as well as therapeutic targets. A panel of nanoparticles for detection of a wide range of diseases and disorders and determination of disease states in a subject is provided. Creating and characterizing protein corona has also been performed in the field, but most of the experimentation has been done using nonmagnetic particles, such as liposome and polymeric nanoparticles, or other particle types that may be used for targeted drug delivery.SELECTED DRAWING: None

Disclosed herein are aspects of magnetic beads that include functional groups suitable for binding biological molecules, such as peptides, polypeptides, proteins, RNA and / or DNA. The magnetic beads may comprise a vinyl azlactone-based crosslinked polymer and a magnetic material dispersed in the bead. In some aspects, the magnetic beads may be used to isolate and / or separate biological molecules, such as from a lysate, or for cleaning up biological molecules suitable for analysis, such as by mass spectrometry.

The present invention relates to a method for detecting cellular or molecular analytes, which method uses two types of engineered phages: a "capture" phage (bait phage) and a "signal"phage (reporter phage). Through this method it is possible to selectively bind a target analyte and thus recognize said target analyte.

The present invention relates to diagnostic test and technology. In particular, the present invention relates to a method for determining an analyte suspected to be present in a sample comprising contacting said sample with a sensor element comprising an anchor layer which is present on a solid support, a first binding agent which is capable of specifically binding to the analyte, which is anchored in the anchor layer and which comprises at least one magnetic label, wherein said at least one magnetic label is located within the anchor layer, a second binding agent which is capable of specifically binding to the analyte when bound to the first binding agent and which is immobilized on the solid support, and a magnetic tunnel junction in functional proximity to the second binding agent which generates a signal elicited in proximity to the at least one magnetic label of the first binding agent, for a time and under conditions which allow for specific binding of the analyte suspected to be present in the sample to the first binding agent and specific binding of the second binding agent to the analyte bound to the first binding agent and detecting the formation the complex of first binding agent, analyte and second binding agent based on the signal which is generated by the magnetic tunnel junction whereby the analyte is determined. Moreover, provided is a device for determining an analyte suspected to be present in a sample and the use thereof for determining an analyte suspected to be present in a sample in said sample. Moreover, the present invention contemplates a kit for determining an analyte suspected to be present in a sample.

This invention provides a novel synthesis method for producing spherical monodisperse polymer beads that have adjustable diameter, good rigidity and good resistance to organic solvents, and contain sufficient hydrophilic functional groups to promote the uploading of a high percentage of superparamagnetic Fe3O4NP, enabling the production of new monodisperse superparamagnetic polymer beads in the 1-5 μm diameter range for IVD applications. [Solution] A monodisperse superparamagnetic bead having a core-shell structure. The bead includes: a core portion formed from a polystyrene polymer matrix material encapsulating a first batch of superparamagnetic Fe3O4 nanoparticles; a first shell portion directly located on the top of the core portion and formed from a crosslinked polymer matrix material composed of a styrene monomer, a crosslinkable monomer, and a first functional monomer having a functional group; and a second shell portion directly located on the top of the first shell portion.

Provided herein are coded microcarriers for analyte detection in multiplex assays. The microcarriers are coded with an analog code for identification and include a capture agent for analyte detection and a substantially transparent magnetic polymer. The analog code is generated by a two-dimensional shape of a substantially opaque layer. Also provided are methods of making the coded microcarriers disclosed herein. Further provided are methods and kits for performing multiplex assays using the microcarriers described herein.

Disclosed herein are aspects of magnetic beads that include functional groups suitable for binding biological molecules, such as peptides, polypeptides, proteins, RNA and / or DNA. The magnetic beads may comprise a vinyl azlactone-based crosslinked polymer and a magnetic material dispersed in the bead. In some aspects, the magnetic beads may be used to isolate and / or separate biological molecules, such as from a lysate, or for cleaning up biological molecules suitable for analysis, such as by mass spectrometry.

Provided herein are coded microcarriers for analyte detection in multiplex assays. The microcarriers are coded with an analog code for identification and include a capture agent for analyte detection and a substantially transparent magnetic polymer. The analog code is generated by a two-dimensional shape of a substantially opaque layer. Also provided are methods of making the coded microcarriers disclosed herein. Further provided are methods and kits for performing multiplex assays using the microcarriers described herein.

Provided herein are encoded microcarriers for analyte detection in multiplex assays. The microcarriers are encoded with an analog code for identification and comprise a capture agent for analyte detection and a substantially transparent magnetic polymer. The analog code is generated by a two-dimensional shape of a substantially non-transparent layer. Also provided are methods of making the encoded microcarriers disclosed herein. Further provided are methods and kits for conducting a multiplex assay using the microcarriers described herein.

The invention relates to methods for conducting binding assays in an assay device that includes one or more storage and use zone. The storage zones of the assay device are configured to house one or more reagents used in an assay conducted in the use zone of the device.

Disclosed herein are various aspects of magnetic beads, including functional groups suitable for binding biomolecules, such as peptides, polypeptides, proteins, RNA, and / or DNA. The magnetic beads may include a vinyl azalactone-based cross-linked polymer and a magnetic material dispersed in the magnetic beads. In certain aspects, the magnetic beads may be used to separate biomolecules, such as from lysates, or to purify biomolecules suitable for analysis, such as mass spectrometry.

The invention relates to methods for conducting binding assays in an assay device that includes one or more storage and use zone. The storage zones of the assay device are configured to house one or more reagents used in an assay conducted in the use zone of the device.

The present invention provides a method that makes it possible to efficiently harvest extracellular vesicles, the method being an alternative to conventional techniques. More specifically, the present invention provides an extracellular vesicle harvesting method that includes the following steps (a) and (b). Step (a): Mixing (i) an extracellular vesicle-containing specimen, (ii) particles with a substance immobilized thereon, the substance having affinity with extracellular vesicles, and (iii) a polymer, to obtain a mixed solution that includes (i') target particles bonded to extracellular vesicles via said substance and (ii') the polymer. Step (b): Isolating the target particles from the mixed solution.　It is preferable that a step for lowering the viscosity of the mixed solution is further included between the steps (a) and (b).

This detection method involves: forming a complex by binding a target substance (11) and dielectric particles (12a) modified with a single domain antibody that binds to the target substance (11); separating the complex and unbound particles (12) that are dielectric particles (12a) not forming the complex by means of dielectrophoresis in a liquid; and detecting the target substance (11) included in the separated complex by using an imaging element (140).

The present invention addresses the problem of providing: a method for simply and accurately detecting a specific protein contained in extracellular vesicles; and a membrane permeabilization treatment agent that can be used in the method. A method for detecting a specific protein contained in extracellular vesicles, the method comprising (A) a step for capturing extracellular vesicles using a carrier capable of binding to an extracellular vesicle-specific marker which exists on the surface of extracellular vesicles, (B) a step for performing a membrane permeabilization treatment on extracellular vesicles captured by the carrier using a membrane permeabilization treatment agent, and (C) a step for introducing a reagent capable of detecting a specific protein contained in extracellular vesicles, into the extracellular vesicles subjected to the membrane permeabilization treatment, wherein the step (B) is carried out such that the specific protein is prevented from leaking off the extracellular vesicles, and the reagent can be introduced into the extracellular vesicles.

The present invention relates to the isolation of selected populations of exosomes with high specificity, thereby enabling accurate determination of exosomal protein content useful for predicting and identifying subjects with Parkinson's disease, and distinguishing Parkinson's disease from atypical parkinsonism, including MSA.

The present invention relates to the use of serum exosomal proteins, alpha-synuclein and clusterin, as biomarkers in the prediction and identification of subjects with Parkinson's disease, and provides methods for measuring their levels. The biomarkers are also useful in monitoring, preventing, and / or treating Parkinson's disease, and in distinguishing Parkinson's disease from atypical parkinsonism, including MSA.

Multiplex analysis methods and systems for accurately detecting and quantitating multiple analytes in a sample are disclosed. The sample is contacted with an analyte capturing agent and immobilized on a magnetic particle, followed by magnetic separation and washing of the particle and bound analyte. This complex is then contacted with a detection agent labeled with an oligonucleotide barcode specific to the analyte target, followed by quantitative measurement of the barcode by qPCR or NGS. The combination of a plurality of analyte capture particles and cognate detection probes allows multiple analytes to be assayed simultaneously and in a multiplex manner.

Providing compositions, methods, and systems for protein corona analysis, as well as their uses. [Solution] Compositions, methods, and systems for analyzing protein coronas are described herein, along with their applications to the discovery of therapeutic targets as well as advanced diagnostic tools. The present invention provides a panel of nanoparticles for the detection of a wide range of diseases and disorders in a subject and for the determination of disease conditions. While the creation and characterization of protein coronas have been carried out in the art, the majority of experiments have been performed using non-magnetic particles, such as liposomes and polymer nanoparticles, or other particle types that can be used for targeted drug delivery.

The present invention relates to diagnostic tests and diagnostic techniques. In particular, the present invention relates to a method for determining an analyte suspected to be present in a sample, the sample being subjected to a sensor element comprising an anchor layer present on a solid support, a first binding agent anchored to the anchor layer and comprising at least one magnetic label and capable of specifically binding to the analyte, the at least one magnetic label being located within the anchor layer, a second binding agent immobilized on the solid support and capable of specifically binding to the analyte when bound to the first binding agent, and a second binding agent induced in proximity to the at least one magnetic label of the first binding agent. The present invention relates to a method for determining an analyte suspected to be present in a sample, comprising contacting a sensor element comprising a magnetic tunnel junction in functional proximity to a second binding agent that generates a signal corresponding to the first binding agent, for a time and under conditions that allow specific binding of an analyte suspected to be present in the sample to the first binding agent and specific binding of the second binding agent to the analyte bound to the first binding agent, and detecting the formation of a complex of the first binding agent, the analyte and the second binding agent based on a signal generated by the magnetic tunnel junction, whereby the analyte is determined. Further provided is an apparatus for determining an analyte suspected to be present in a sample and its use in said sample for determining an analyte suspected to be present in the sample. Furthermore, the present invention contemplates a kit for determining an analyte suspected to be present in a sample.

Compositions, methods, and systems for analyzing the protein corona are described herein, as well as its application in the discovery of advanced diagnostic tools as well as therapeutic targets.

Provided herein are methods for detecting and identifying disease-specific biomarkers, such as target antigens associated with infection.

Compositions, methods, and systems for analyzing the protein corona are described herein, as well as its application in the discovery of advanced diagnostic tools as well as therapeutic targets.

The present invention relates to a method for detecting cellular or molecular analytes, which uses two types of artificial phages, a "capture" phage (bait phage) and a "signal" phage (reporter phage), that are capable of selectively binding and thus recognizing the target analyte.

The invention relates to the field of biomolecule detection methods, in particular to magnetic gold nanoparticles and a green preparation method and application thereof. The preparation method comprises the following steps: adding water into tea leaves and heating; filtering and collecting filtrate; preparing a thiol modified Fe2O3 suspension liquid; preparing magnetic gold nanoparticle turbid liquid; the application method comprises the following steps: adding the capture antibody into magnetic gold nanoparticle turbid liquid; adsorbing with a magnet, and removing supernate; adding confining liquid; washing is performed; adding biomolecules to be detected; adsorbing with a magnet, and removing supernate; adding a detection antibody; adsorbing with a magnet, and removing supernate; streptavidin marked with enzyme is added; adsorbing with a magnet, and removing supernate; adding a chemical fluorescent substrate; and adding a stop solution, and detecting the absorbance of the solution. The method not only solves the problems of more impurities, larger particle size change and complicated centrifugal treatment in ELISA (enzyme-linked immuno sorbent assay) detection of the non-magnetic gold nanoparticles prepared by the existing method, but also can be further applied to the future chip ELISA biomolecular detection.