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Pyemia early diagnosis liquid phase chip and method for producing the same

An early diagnosis, liquid-phase chip technology, applied in measurement devices, instruments, scientific instruments, etc., can solve the problems of inability to distinguish bacterial and viral infections, low sensitivity and specificity of sepsis, and achieve a simple and easy preparation method. , high sensitivity and specificity

Inactive Publication Date: 2008-08-20
SUREXAM BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0014] The purpose of the present invention is to provide a method for detecting, prognosing and evaluating the efficacy of sepsis, which has low sensitivity and specificity, cannot distinguish between bacterial and viral infections, and can only detect one marker in one reaction. Liquid chip for early diagnosis of sepsis with joint detection of four markers

Method used

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  • Pyemia early diagnosis liquid phase chip and method for producing the same
  • Pyemia early diagnosis liquid phase chip and method for producing the same
  • Pyemia early diagnosis liquid phase chip and method for producing the same

Examples

Experimental program
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Effect test

Embodiment 1

[0061] Example 1 Preparation of Liquid Chip for Early Diagnosis of Sepsis and Detection of Antigen

[0062] The capture antibody of the present invention is a monoclonal antibody that can specifically bind to CRP, PCT, IL-6, and neopterin respectively;

[0063] The CRP capture antibody used in this example is the monoclonal antibody of CRP, the clone number is C2, the detection antibody is the monoclonal antibody of CRP, the clone number is C6, all purchased from Hytest Company; the PCT capture antibody is the monoclonal antibody of PCT, The clone number is 14C12, the detection antibody is the monoclonal antibody of PCT, the clone number is 38F11, all purchased from Hytest Company; the IL-6 capture antibody is the monoclonal antibody of IL-6, the clone number is 6708, purchased from R&D Company, and the detection antibody The antibody is a polyclonal antibody of IL-6, purchased from abcam company; the capture antibody of neopterin is a monoclonal antibody of neopterin, the clo...

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PUM

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Abstract

The invention discloses a sepsis early diagnosis liquid chip which mainly includes the following components: microsphere coated with PCT capturing antibody, microsphere coated with CRP capturing antibody, microsphere coated with IL-6 capturing antibody, microsphere coated with Neopterin capturing antibody, these microspheres have codes with different colors; biotin-labeled test antibody; avidin-linked phycoerythrin. The invention sepsis early diagnosis liquid chip has advantages of high detection efficiency, a small amount of sample, high specificity and high sensitivity. And at the same time, the invention can test four kinds of specific markers of sepsis simultaneously, improve sensitivity and specificity of sepsis early diagnosis, distinguish sepsis caused by bacterial and viral, judge severity and poor prognosis of sepsis and monitor continuously for judging reflection of patient to certain kind of treatment.

Description

technical field [0001] The invention relates to medical in vitro diagnostic technology, in particular to a sepsis early diagnosis liquid phase chip and a preparation method thereof. Background technique [0002] Sepsis is a common complication after severe infection, severe trauma (burn), shock, and surgery. It is a severe systemic inflammation induced by microorganisms (such as bacteria, viruses, fungi, parasites, etc.) invading the human body. A set of clinical manifestations of pathophysiological processes that respond and are damaging to tissues. Severe sepsis can lead to septic shock and multiple organ dysfunction (MODS), which poses a great threat and challenge to human health and economic development, and has become the main cause of non-cardiac death. [0003] Currently, sepsis has three high phenomena: high morbidity, high mortality, and high treatment costs. According to statistics, the prevalence of sepsis is about 0.3% of the total population, and the total num...

Claims

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Application Information

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IPC IPC(8): G01N33/543
Inventor 许嘉森林一群谢建平
Owner SUREXAM BIO TECH
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