50results about How to "Use less blood" patented technology

The invention provides a microfluidic chip and a circulating tumor cell capture method using the same. The microfluidic chip comprises a separating cavity, a micro-pillar array and an external magnetic field assembly, wherein a sample injection opening is formed in the middle of a first side of the separating cavity; a to-be-detected particle enrichment part and a sample outflow opening are respectively arranged at the upper part and the lower part of a second side; the micro-pillar array is arranged in the separating cavity and between the sample injection opening and the sample outflow opening; the external magnetic field assembly is used for forming a magnetic field inside the separating cavity from the bottom up; among samples injected from the sample injection opening, to-be-detected particles of which the surfaces are connected with magnetic beads move upwards under the application force of the magnetic field, and are enriched at the to-be-detected particle enrichment part or attached onto the top wall of the separating cavity, and to-be-detected particles of which the surfaces are not connected with magnetic beads are intercepted and captured by the micro-pillar array; besides the to-be-detected particles, other components in the samples penetrate through the micro-pillar array under the gravity action. According to the method, two capture modes are combined, so that high capture rate and high throughput are realized, and high-purity circulating tumor cells are captured.

The invention belongs to the technology field of the cellular immunology, and particularly relates to a method for the dominant amplification of in vitro peripheral blood nature killer cells with a large amount. The method comprises the steps as follows: (1) separating single nucleus cell from human or animal peripheral blood; (2) suspending the single nucleus cell in an RPMI 1640 culture solution containing 5%-15% autologous serum and pretreating with methyl-Beta-cyclodextrin with an effective concentration of 1-4mmol/L for 36-60 hours; and (3) adding recombined interleukin 2, and amplification-culturing in an RPMI 1640 culture solution containing 5% of new-born calf serum and 5% of autologous serum for above 10 days. The method has the advantages that the blood sample amount is small; the cost is low; the operation is convenient; and the amplification multiple is high, and the nature killer cells can be amplified by 392-1752 times in a short term.

The invention discloses a kit for detecting TORCH IgM antibodies. The kit comprises a solid carrier which is coated with an antibody combined with the TORCH IgM antibodies, a TORCH antigen which is marked with lanthanide, a TORCH IgM antibody calibrator, a sample diluent, an experiment buffering solution, a concentration cleaning solution and an enhancement solution. The kit can simultaneously detect multiple pathogene IgM antibodies in TORCH pathogene under the same detection condition and is high in sensitivity, high in specificity, good in precision, high in accuracy, little in blood consumption amount, rapid, convenient and favorable for early diagnosis on the infection of the TORCH pathogene. The method has the characteristic that the detection linear range is wide, the diluting times or diluting ratio of a high-value sample can be reduced, and the accuracy of a detection result can be improved.

The invention relates to a newborn baby TRECs and KRECs gene copy number detection kit using a digital PCR technology. The kit comprises a forward primer, a reverse primer and a probe used for detecting a TRECs gene, a forward primer, a reverse primer and a probe used for detecting a KRECs gene, and a forward primer, a reverse primer and a probe used for detecting a reference gene TRAC. The detection of the project can be completed only through hole forming to drill a round dried blood spot (with the blood quantity about 3mul) with the diameter being 3mm; the blood consumption is low; the kitis very suitable for the newborn baby screening unsuitable for great blood sampling quantity. Two rounds of sequential detection strategy are used; in the first round of detection, only the synchronous detection on the TRECs and KRECs gene copy number by single hole is needed; the two results are quality control reference indexes for each other. The second round of reference gene TRAC copy numberdetection is needed only when the TRECs and KRECs gene copy numbers are lower than the threshold values at the same time; whether the detection control loss due to sample quality problem occurs or notis determined.

The invention belongs to the technical field of medical instruments, and particularly relates to a disposable hard connecting band collection container and an anti-freezing vacuum blood taking needle of a bleeding opening. The disposable hard connecting band collection container is formed in the following mode that: an anticoagulant coating layer is arranged on an inner cavity wall of a circular connector; one end of the connector is provided with a venous blood taking needle; the rear part of the connector is provided with a vacuum valve; the other end of the connector is provided with the blood collection sample bottle needle; the blood collection sample bottle needle is connected with a blood collection sample bottle cap; the blood collection sample bottle cap is connected with a rubber plug; the rubber plug is connected with a blood collection sample bottle; and one side on the blood collection sample bottle close to the blood collection sample bottle cap is provided with one secondary bleeding opening. The disposable hard connecting band collection container and the anti-freezing vacuum blood taking needle of the bleeding opening have the advantages that: the anticoagulant coating layer is added on the basis of the conventional hard connection type blood taking needle so that blood flowing out of a body can contact an anticoagulant; a blood sample is ensured to generate no cruor and hemolysis so as to ensure the accuracy of the test result; and the secondary bleeding opening is added so that a clinical inspector can flexibly control the blood dosage during the test to ensure repeated tests at any time or add new testing items.

The invention belongs to the field of molecular cytogenetics and relates to an amplified composition for fast gene detection of microdeletion of Y chromosomes, a kit containing the same and an application thereof. The amplified composition comprises a group A and a group B, wherein each group comprises a pair of primers respectively in AZFa, AZFb and AZFc areas and two pairs of internal control primers ZFY and SRY; the primers in the group A have the nucleotide sequence shown in SEQ ID No.1-1O; the primers in the group B have the nucleotide sequence shown in SEQ ID No.11-2O. The amplified composition, the kit and the application have the advantages that on the basis of the prior art, multiple PCR (Polymerase Chain Reaction) buffering liquid and Tag polymerase are improved, and trace whole-blood amplification is directly adopted due to less blood consumption, so that the pain of a patient is reduced; and the procedure of extracting DNA is omitted, so that the time and the labor are saved and the economic expenditure is greatly reduced.

The invention discloses a reagent for detecting anti-platelet surface receptor-specific autoantibodies and a preparation method and application thereof. The reagent has high specificity and sensitivity. The method comprises expressing a human platelet-specific receptor membrane glycoprotein GP Ib-IX and/or GP IIb/IIIa receptor through CHO cells, carrying out co-incubation on the specific membraneglycoprotein receptor expression-positive CHO cells and serum to be detected and adding a fluorescein-labeled anti-human immunoglobulin polyclonal antibody (secondary antibody) for bonding. If there is an anti-platelet receptor-specific antibody in serum or plasma, the CHO-membrane glycoprotein specific antibody-fluorescein-labeled anti-human immunoglobulin polyclonal antibody complex structure isformed, has high CHO cell fluorescence intensity and can be used for flow cytometry or fluorescence microscope examination. The method is easy to operate, has enough specific membrane glycoprotein expression positive CHO cell sources, high specificity and sensitivity and high diagnosis efficiency and can be used for basic research and clinical examination.

The invention relates to a real-time fluorescent quantitative PCR kit for detecting TRECs and KRECs genes. The kit comprises: 1) a DNA extraction reagent; 2) a standard product containing a TRECs geneinsertion sequence, a KRECs gene insertion sequence and a TRAC gene insertion sequence; 3) a fluorescent PCR reaction solution I containing fluorescent PCR primers and detection probes of the TRECs gene and the TRAC gene; and 4) a fluorescent PCR reaction solution II containing fluorescent PCR primers and detection probes of the KRECs gene and the TRAC gene. The kit has the advantages of high sensitivity, good specificity, simple and rapid detection method, reliable experimental result, realization of screening of immunodeficiency diseases mainly including SCID and antibody deficiency, provision of clinically relevant indications for other primitive immunodeficiency diseases related to T cell and B cell development or other systemic diseases, and facilitation of early diagnosis and treatment.

The invention relates to a reagent and a test cup used for rapid detection of blood viscoelasticity. The reagent comprises solvent water, 2 to 8g/L of a buffering agent, 0.1 to 1g/L of NaOH, 0.1 to 0.5g/L of propyl gallate, and 10 to 30g/L of CaCl2, and the pH value ranges from 6.0 to 9.0. According to the reagent, propyl gallate is taken as an activator, is capable of activating platelet membranephosphatidylserine (PF3, blood clotting catalytic surface) internal and external membrane turnover, activating an endogenous blood clotting system at the same, and starting blood clotting process inthe presence of calcium ions. After treatment, propyl gallate is capable of dissolving in water, so that uniform mixing in reaction can be ensured, and propyl gallate is not influenced by aprotinins .

The invention relates to a real-time fluorescent quantitative PCR (polymerase chain reaction) kit for one-step quantitative detection of KRECs gene and its application. The kit comprises a real-time fluorescent quantitative PCR reaction system based on real-time fluorescent PCR technique. The fluorescent PCR reaction system comprises forward and reverse primers specific to KRECs and beta-actin genes, and a specific fluorescent probe. The kit allows quick screening of neonatal immune system B-cell level, has high sensitivity and stability and provides excellent reproducibility, and this method is applicable to the quantitative detection of KRECs and to the functional screening of the neonatal immune system and is worthy of practical clinical application.

The invention discloses a device of extracorporally imitating blood pressure fluctuation rising and an application method and an application, and relates to the field of medical research tools. An imitation blood pressure pump and an imitation blood pressure fluctuation pump are respectively arranged, so that imitation blood pressure and blood pressure fluctuation change are suffered from the adjustment of different regulating units. Separating adjusting effects of the imitation blood pressure and blood pressure fluctuation change are achieved. The device of extracorporally imitating blood pressure fluctuation rising solves the problem that no proper extracorporal model for studying the blood pressure fluctuation rising exists.

The invention provides a device for connecting artificial blood vessels with aortas and innominate artery blood vessels in operations. The device comprises a connecting device body which is of a tubular structure, the inner side wall of the connecting device body is smooth, and a groove is formed in the outer side of the tubular structure along the circumference. According to the device, a traditional suturing technology can be replaced, the operation and deep hypothermic circulatory arrest time can be effectively shortened, and complications such as anastomosis bleeding are avoided, so that the blood consumption is greatly reduced, and the operation survival rate is increased.

The invention discloses an electrochemical biosensor. The electrochemical biosensor comprises a substrate layer, an electrode output end is arranged at the upper end of the substrate layer, a reactionelectrode and an auxiliary electrode are arranged at the lower end of the substrate layer, a conductive circuit is connected between the reaction electrode, the auxiliary electrode and the electrodeoutput end, a hydrophilic film layer is arranged on a reaction channel at the lower end of the substrate layer, and a to-be-detected liquid inlet is formed in the front end of the hydrophilic film layer; air holes are respectively formed in the hydrophilic film layer at the rear end of the reaction channel; and reagent layers are arranged on the reaction electrode and the auxiliary electrode. Theinvention also discloses a method for measuring a blood impedance phase angle by using the electrochemical biosensor.

The embodiment of the invention discloses a sample analyzer. The sample analyzer comprises a sample collection and distribution part, a reagent supply part, a test solution preparation part, a detection part, an analysis part and a controller, the embodiment of the invention further discloses a sample analysis method which is applied to the sample analyzer and is used for calculating and determining the target sample amount needing to be sucked according to the detection item or the detection mode input by the user, so that the blood sample sucked according to the constant amount is prevented from being wasted, and the blood use amount in the blood sample analysis process is reduced; the invention further discloses an animal blood sample analyzer applying the scheme.

The invention discloses a microscale serum insulin content detection device which includes a gas-control unit, a microfluidic integrated chip and a detection apparatus. The microfluidic integrated chip includes a glass base layer, a gas driving layer and a liquid flowing layer which are installed together in a successive manner from bottom to top. A mixer, a plurality of gas channel pipes, a plurality of structure gas chambers and a plurality of pneumatic micropumps are arranged on the gas driving layer and are communicated with each other. The gas channel pipes are communicated with the gas-control unit. A mixing pool, a plurality of fluid channels and a plurality of micro liquid storage pools are arranged on the liquid flowing layer, wherein the number of the fluid channels and the number of the micro liquid storage pools are corresponding to the number of the structure gas chambers. Each fluid channel is communicated with each micro liquid storage pool. The detection apparatus includes a photomultiplier. The device is small in size, little in blood consumption, low in cost, high in detection speed and high in sensitivity. The device has great significances of achieving popularized diabetes screening and early-stage diagnosis and medical treatment and reducing harms of large-scale epidemic diseases on human society.