Reagent and test cup used for rapid detection of blood viscoelasticity

A viscoelasticity and reagent technology, applied in the field of inspection, can solve the problems of poor repeatability and unevenness of agglutination reaction, and achieve the effects of reducing blood consumption, avoiding test errors and improving stability

Active Publication Date: 2018-07-13
GUANGZHOU WONDFO BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The ordinary cup of thromboelastography uses kaolin as the activator. After the reagent and the blood sample are activated, there may be a phenomenon of sedimentation, and kaolin is a solid, and there may be unevenness when it is premixed with blood, resulting in poor repeatability of the agglutination reaction.

Method used

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  • Reagent and test cup used for rapid detection of blood viscoelasticity
  • Reagent and test cup used for rapid detection of blood viscoelasticity
  • Reagent and test cup used for rapid detection of blood viscoelasticity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The present embodiment implements a reagent for rapid detection of blood viscoelasticity, which is prepared as follows:

[0034] Add 0.1g NaOH to 1000mL buffer solution (2g MOPS), stir for 10min until the solid dissolves, add 0.1g propyl gallate, and ultrasonicate the mixed solution for 20min to make it fully mixed. Then add 10 g of anhydrous CaCl 2 , 0.01g Tx-100, 0.5g trehalose, 1g glycine, 0.005g BHA and 0.005g sodium azide. Stirring was continued for 30 min until the solid was dissolved, and the pH of the reagent was adjusted to 6.0.

[0035] Then pipette 1uL of the reagent and dispense it on the bottom of the test cup, and place the subpackaged test cup in an oven at 50°C for 30 minutes to dry. The baked test cup is taken out to be a test cup capable of accurately and quickly detecting the viscoelasticity of whole blood.

Embodiment 2

[0037] The present embodiment implements a reagent for rapid detection of blood viscoelasticity, which is prepared as follows:

[0038] Add 0.5g NaOH to 1000mL buffer solution (4g MOPS), stir for 10min until the solid dissolves, add 0.2g propyl gallate, and ultrasonicate the mixed solution for 20min to make it fully mixed. Then add 20 g of anhydrous CaCl 2 , 0.08g Tx-100, 2.5g trehalose, 5g glycine, 0.01g BHA and 0.01g sodium azide. Stirring was continued for 30 min until the solid was dissolved, and the pH of the reagent was adjusted to 7.0.

[0039] Then pipette 5uL of the reagent and dispense it on the bottom of the test cup, and place the subpackaged test cup in an oven at 50°C for 30 minutes to dry. The baked test cup is taken out to be a test cup capable of accurately and quickly detecting the viscoelasticity of whole blood.

Embodiment 3

[0041] The present embodiment implements a reagent for rapid detection of blood viscoelasticity, which is prepared as follows:

[0042] Add 1g NaOH to 1000mL buffer solution (8g MOPS), stir for 10min until the solid dissolves, add 0.5g propyl gallate, and ultrasonicate the mixed solution for 20min to make it fully mixed. Then add 30 g of anhydrous CaCl 2 , 0.1g Tx-100, 5g trehalose, 10g glycine, 0.02g BHA and 0.02g sodium azide. Stirring was continued for 30 min until the solid was dissolved, and the pH of the reagent was adjusted to 9.0.

[0043] Then pipette 20uL of the reagent and dispense it on the bottom of the test cup, and place the subpackaged test cup in an oven at 50°C for 30 minutes to dry. The baked test cup is taken out to be a test cup capable of accurately and quickly detecting the viscoelasticity of whole blood.

[0044] The quality control plasma purchased from Heamoscope was used in the test, and the reference range is shown in the table below:

[0045] ...

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PUM

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Abstract

The invention relates to a reagent and a test cup used for rapid detection of blood viscoelasticity. The reagent comprises solvent water, 2 to 8g/L of a buffering agent, 0.1 to 1g/L of NaOH, 0.1 to 0.5g/L of propyl gallate, and 10 to 30g/L of CaCl2, and the pH value ranges from 6.0 to 9.0. According to the reagent, propyl gallate is taken as an activator, is capable of activating platelet membranephosphatidylserine (PF3, blood clotting catalytic surface) internal and external membrane turnover, activating an endogenous blood clotting system at the same, and starting blood clotting process inthe presence of calcium ions. After treatment, propyl gallate is capable of dissolving in water, so that uniform mixing in reaction can be ensured, and propyl gallate is not influenced by aprotinins .

Description

technical field [0001] The invention relates to the technical field of inspection, in particular to a reagent and a test cup for rapidly detecting blood viscoelasticity. Background technique [0002] Thromboelastography (TEG) can provide all information from coagulation initiation to platelet association formation, fibrin filament formation, clot growth, maximum clot formation, clot degradation to dissolution, and is a new coagulation detection mode based on cytology . TEG has now become an important indicator for perioperative monitoring of coagulation function, and is gradually widely used in liver transplantation, cardiac surgery and other fields, and guides intraoperative blood transfusion, medication, diagnosis, thrombolysis and anticoagulant treatment, and evaluates the risk of deep vein thrombosis . [0003] Thromboelastography is mainly used to detect the physical characteristics of blood clots, that is, the time of the first blood clot formation, the kinetic chara...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/86G01N11/00B01L3/00
CPCB01L3/50G01N11/00G01N33/86
Inventor 周杨美慧娄长春彭统全王继华
Owner GUANGZHOU WONDFO BIOTECH
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