Microfluidic chip and circulating tumor cell capture method using same

Abstract

The invention provides a microfluidic chip and a circulating tumor cell capture method using the same. The microfluidic chip comprises a separating cavity, a micro-pillar array and an external magnetic field assembly, wherein a sample injection opening is formed in the middle of a first side of the separating cavity; a to-be-detected particle enrichment part and a sample outflow opening are respectively arranged at the upper part and the lower part of a second side; the micro-pillar array is arranged in the separating cavity and between the sample injection opening and the sample outflow opening; the external magnetic field assembly is used for forming a magnetic field inside the separating cavity from the bottom up; among samples injected from the sample injection opening, to-be-detected particles of which the surfaces are connected with magnetic beads move upwards under the application force of the magnetic field, and are enriched at the to-be-detected particle enrichment part or attached onto the top wall of the separating cavity, and to-be-detected particles of which the surfaces are not connected with magnetic beads are intercepted and captured by the micro-pillar array; besides the to-be-detected particles, other components in the samples penetrate through the micro-pillar array under the gravity action. According to the method, two capture modes are combined, so that high capture rate and high throughput are realized, and high-purity circulating tumor cells are captured.

Description

technical field [0001] The invention relates to the technical field of microfluidic chips and medical detection, in particular to a microfluidic chip and a method for capturing circulating tumor cells using it. Background technique [0002] With the progress of society, the problems of life and health are increasingly attracting people's attention. Cancer is a major problem affecting human life and health. How to overcome this difficult problem and make people treat cancer like a cold is the subject and direction that human beings are striving for. Traditional methods of surgery, chemotherapy, and radiotherapy are costly, time-consuming, and complicated, and bring great pain to patients. Circulating tumor cells (CTCs) are tumor cells that survive in the blood circulation system during tumor metastasis. The detection of circulating tumor cells (CTCs) is helpful for the early detection of tumor micrometastases. And the number of CTCs is closely related to the severity of c...

AI Technical Summary

Problems solved by technology

This method also has the defects of semi-automatic, high cost and low efficiency

The false positive rate is high, the enriched CTCs have no biological activity, only positive for EpCAM, and cannot detect malignant CTCs that do not express EpCAM

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