Micro-flow controlled chip used for detecting erythrocyte osmotic fragility

Abstract

The invention relates to a micro-fluidic chip used for testing erythrocyte osmotic fragility, which comprises a chip main-body, wherein, two solution inlets and a blood sample inlet are formed on the chip main-body; and a plurality of detection pools arranged inside the chip main-body are communicated with the blood sample inlet through a blood sample microchannel and communicated with the two solution inlets through a microchannel network that generates concentration gradient automatically. Based on the micro-fluidic chip, a test method of erythrocyte osmotic fragility comprises the steps asfollows: (1) blood samples are introduced into the different detection pools in the micro-fluidic chip through the blood sample inlet; (2) two NaCl solutions with different concentrations are introduced into the micro-fluidic chip through the two solution inlets at the same flow speed at the same time, and mixed with the blood samples in the detection pools; (3) erythrocytes in the detection pools are observed through a microscope and are shoot; and (4) the intact erythrocytes in each detection pool are distinguished in erythrocyte pictures and counted. The micro-fluidic chip reduces use of blood, reduces manual intervention, and has high detection speed and objective and accurate detection results.

Description

technical field [0001] The invention relates to a red blood cell osmotic fragility testing technology, in particular to a microfluidic chip capable of automatically generating concentration gradients and a microfluidic chip-based red blood cell osmotic fragility testing method. Background technique [0002] The characteristic of hemolysis of red blood cells in hypotonic saline solution is called osmotic fragility of red blood cells, which is an important clinical hematology test index. Under normal conditions, the osmotic pressure in red blood cells is approximately equal to the plasma osmotic pressure, and red blood cells can maintain normal size and shape when placed in isotonic solution (0.9% NaCl); if red blood cells are placed in hypertonic solution (>0.9%) , the water will escape out of the cell, and the cells will shrink due to the loss of water; if the red blood cells are placed in a hypotonic solution (<0.9%), water will enter the cells, and the red blood cell...

AI Technical Summary

Problems solved by technology

The conventional inspection operation method needs to manually prepare 12 different concentrations of NaCl solutions, and use normal human blood samples and patient blood samples for comparison tests. After standing for 2 hours, the inspection results can be obtained by naked eye observation.

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