The invention relates to a melamine colloidal gold test card as well as a production and application method thereof, belonging to the technical field of immunology. The rapid diagnosis card comprises a shell and a test paper strip and is characterized in that the shell is provided with a sample application hole and an observation window, the test paper strip is arranged in the shell, a cellulose nitrate film is superposed in the middle part of a polyvinyl chloride (PVC) liner plate, a water-absorbing pad and a sample pad are superposed at both ends of the PVC liner plate, the cellulose nitrate film overlaps with the water-absorbing pad and the sample pad, a length of film containing a melamine monoclonal antibody immune colloidal gold compound is attached to a sample area, and the cellulose nitrate film is provided with a test tape containing melamine coating antigens and a quality control tape containing goat-anti-mouse IgG. The test card of the invention is a rapid, accurate and sensitive diagnoses card, does not need any special instrument, has the advantage of simple operation and can meet the test requirements of food safety, animal husbandry and testing organization.