144results about How to "Improve positive detection rate" patented technology

The invention relates to a performance test device for a water immersion focusing probe and a test method thereof. A steel ball is placed on a reference reflection seat of the performance test device placed in a water tank, the corresponding upside of the steel ball is provided and aligned with a probe to be tested, the probe to be tested generates ultrasonic signals to the steel ball through an ultrasonic gauge, and the performance difference of the probe is tested through the variation of reflection signals. A three-dimensional guide adjusting device is adjusted to test the position and variable quantity of the probe in real time so that reflection echo signals of the probe on the steel ball reaches maximum. Effective reflected wave is received by the probe and displayed on a display screen of the ultrasonic gauge in a form of wave height; scale numerical values on X axis, Y axis and Z axis of a graduated scale are recorded respectively; and various performance indexes of the probe are determined by observing the variation of the wave height. The performance test device for the water immersion focusing probe and the test method thereof are simple and practical, and can test the indexes such as focus, sound width, focus diameter, sound offset angle, sensitivity surplus, sensitivity deflection of a multi-chip probe and the like without depending on special laboratory equipment.

The invention belongs to the field of biomedicine examination, and particularly relates to a kit and a method for detecting mycobacterium tuberculosis infection and application. The invention discloses a novel mycobacterium tuberculosis detection reagent by screening specific T cell epitope of mycobacterium tuberculosis, wherein the reagent contains polypeptide or analog thereof represented by SEQ ID No.1-10. The method detects cell factors released from T cells by using single or more SEQ ID No.1-10 polypeptides to contact the T cells of mycobacterium tuberculosis infected individuals. The method can effectively detect active tuberculosis or latent tuberculosis infection, and is free from disturbance of Bacilli Calmette Guerin (BCG) inoculation vaccines. The invention also discloses a diagnostic kit and other application based on the polypeptide and the method. Compared with the gamma interferon release experiments in the prior art, the method can obviously improve the detection rate without reducing the specificity and has high clinical application value.

The invention discloses a kit for colorectal cancer detection and an application thereof. The kit comprises a primer composition, and a probe composition. The primer composition comprises BS-S9-F, BS-S9-R, BS-N4-F, BS-N4-R, BS-SDC2-F, BS-SDC2-R, BS-ACT-F and BS-ACT-R. The probe composition comprises BS-S9-P, BS-N4-P, BS-SDC2-P and BS-ACT-P. The kit of the invention can be applied to the detectionof colorectal cancer, and has the advantages of high sensitivity and high specificity.

The invention belongs to the technical field of moving target detection of sequence images, and discloses a moving target rapid detection method based on sequence images, a computer vision system andpreprocessing of the sequence images. The method comprises the following steps of in the acquisition process of the sequence images, adopting image graying, binaryzation and median filtering for processing; for the low-illumination image, adopting Gamma transformation to enhance the image; detecting a moving target in a static environment by combining a detection method of background difference and edge inter-frame difference; and detecting a moving target in a dynamic environment based on a detection method of SIFT algorithm feature matching. The positive detection rate reaches 96.3%, the false detection rate is 1.3%, and compared with a background difference method and an inter-frame difference method, the positive detection rate and the false detection rate are greatly improved; the performance is obviously improved in three aspects of positive detection rate, false detection rate and processing time. Wherein the positive detection rate of the method disclosed by the invention is 92.7%; the false detection rate is 1.9%; wherein the treatment time is 7.2 s.

The invention discloses a preservation solution for exfoliated cell and a preparation method which uses the preservation solution to process cell thin layer smear. The preservation solution for exfoliated cell comprises the components which are prepared by the volume proportion of 10 to 15 portions of methanol, 10 to 15 portions of alcohol, 1 to 2 portions of glycerin, 1 to 2 portions of glycol polyethylene, 10 to 12 portions of formaldehyde, 0.5 to 1 portions of glacial acetic acid, 10 to 20 portions of sodium chloride with 0.85 percent to 0.9 percent of mass concentration and 33 percent to 68.5 percent of phosphate buffer. The steps of the preparation method for cell thin layer smear are that: 1) the collected sample is put in the preservation solution for exfoliated cell for shaking; the sample is transferred to a centrifuge for centrifugal treatment after standing; 2) the supernatant is removed until 1ml to 2ml residue is left in the centrifuge tube; the cell suspension is obtained after uniform vibration; 3) 0.5ml to 1ml cell suspension is put into a smear preparation cup which is made into cell thin layer smear by a cell smear preparation machine.

The invention discloses a method for detecting HBVDNA through combination of PCR and CRISPR. The method comprises the steps of (1) amplifying nucleic acid of samples to be detected through a pair of upstream and downstream specific primers, wherein a sequence which can be recognized and transcribed by T7RNA polymerase is arranged at a 5' end of the downstream primer; and (2) detecting whether a targeting sequence exists or not in amplification products of the nucleic acid of the samples to be detected in a detection system containing crRNA, T7RNA polymerase, Cas13a protein and report RNA for recognizing an HBVDNA target sequence, wherein the section of the HBVDNA target sequence is 803<rd>-829nt. The invention further discloses crRNA capable of targeting HBVDNA specific sites and a reagent kit containing the crRNA. The method provided by the invention is simple, convenient and quick during detection of the content of the HBVDNA, and has extreme high sensitivity and specificity, single-copy HBV DNA is differentiated within 15 minutes after PCR amplification, and higher positive detection rate can be shown for serum samples low in virus load.

The embodiment of the invention relates to clinical in-vitro detection, in particular to a kit for quickly and accurately detecting a novel coronavirus IgM antibody and a preparation method of the kit. The kit provided by the invention comprises: a horseradish peroxidase labeled novel coronavirus antigen; and magnetic particles coupled with an anti-human IgM antibody, the novel coronavirus antigencomprises an N protein antigen derived from a novel coronavirus N protein and an S protein antigen derived from a novel coronavirus S protein. According to the kit provided by the invention, the newcoronavirus IgM antibody is used as a detection object, so that early auxiliary diagnosis on a patient is facilitated; n protein and S protein fragments are jointly used as antigens for combined use,so that the sensitivity of related products can be improved on the basis of not influencing the specificity; complementation with nucleic acid detection can be formed, and the new coronal pneumonia detection rate is increased; the positive detection rate of the kit is obviously higher than that of similar new coronavirus IgM detection products sold in the market, and the kit can more accurately serve the field of in-vitro diagnosis.

The invention belongs to the field of cell treating fluid, and particularly relates to alkali solution based cell preservation treating fluid and a preparation method thereof. The alkali solution based cell preservation treating fluid is mainly composed of an egg white dissolution agent, a red blood cell cracking agent, an alkaline substance, a corrosion remover and distilled water. The alkali solution based cell preservation treating fluid is mainly used in cooperation with a membrane type liquid based thin-layer cell slide preparation machine and can enhance the cell adhesion capability of a modified glass slide, promote cervical cell enrichment so as to firmly attach cervical cells to the specially-prepared glass slide and effectively reduce the phenomenon of leak detection. Meanwhile, the novel alkali solution based cell preservation treating fluid has the advantages of being free of toxicity, environmentally friendly, capable of rapidly and thoroughly achieving sterilization, low in cost, convenient to configure, safe and efficient.

The invention belongs to the technical field of biotechnologies and DNA detection, and particularly relates to a DNA methylation qPCR kit for lung cancer detection and a using method thereof, in particular to a kit using plasma free DNA to conduct methylation of qCPR to determine the methylation states of one or more gene targets (such as SHOX2 and SOX17) for lung cancer detection or screening anda using method of the kit. The kit can significantly increase the positive detection rate of early lung cancer and improve specificity of the early lung cancer through an experimental test, detectionsensitivity of biomarkers can be improved to picogram/nanogram level DNA molecules, and improvement of the detection sensitivity is realized by optimizing specific nucleotide sequence primers and DNAprobes and improving a hydrosulphite treatment method of the plasma free DNA.

The invention relates to a cardiac muscle biopsy system with electrophysiology standard measurement and three-dimensional positioning functions. The cardiac muscle biopsy system comprises a conduit of which the far end has an adjustable bending degree, a pair of forceps at the far end of the conduit, an operating handle at the near end of the conduit, a tail wire connected with the handle and an electrophysiology standard measurement and three-dimensional positioning device, wherein the pair of forceps at the far end of the conduit are made of metal with high electrical conductivity so as to form a far end standard measurement electrode; the near ends of the metal forceps are provided with a plurality of annular electrodes which are made of the metal with the high electrical conductivity;the metal forceps and the annular electrodes are connected to an electrophysiology standard measurement and three-dimensional positioning system by the tail wire; and the electrophysiology standard measurement and three-dimensional positioning system comprises an electrical signal processing device, a positioning signal processing device, a work station and an output device. By the system, normaland abnormal cardiac muscles are identified through electrophysiology standard measurement, the success rate of biopsy is increased, and complicating diseases are reduced.

The invention discloses a novel coronavirus 2019-nCoV nucleic acid kit and a virus nucleic acid acquisition method. The method comprises the following steps: according to novel coronavirus 2019-nCoV ORF1ab and an N gene as amplification target areas, designing specific primers and fluorescent probes for detecting novel coronavirus 2019-nCoV nucleic acid in samples; and performing Digital PCR (polymerase chain reaction) absolute quantification on 2019-nCoV nucleic acid RNA (ribonucleic acid) by using a one-step method by using a prepared Super premix reaction liquid A together with Super premixreaction liquids B and C, and performing Digital PCR detection on RNA of a diluted positive virus sample. The kit and the method have the beneficial effects that by using the novel virus nucleic acidacquisition method, and together with a Digital PCR probe method, the nucleic acid positive detection rate of infection of the novel coronavirus 2019-nCoV can be increased, the process is ensured tobe specified and standardized, the influence of artificial non-standard operation upon detection results can be reduced, result reliability can be ensured, and meanwhile, false negative results causedby detection of sputum and throat swab sampling together with real-time fluorescent PCR methods can be avoided.

The invention discloses a detecting device for Brucella infection. The detecting device comprises a test strip; a Brucella antigen detection area and a Brucella antibody detection area are arranged on a chromatographic membrane of the test strip. According to the detection device, a Brucella antigen and a Brucella antibody in a sample can be detected simultaneously; various outer membrane proteins are applied in a combined manner, so that the occurrence of false positive results can be reduced effectively; a double-antigen sandwich method and a double-antibody sandwich method are adopted, so that the sensitivity and the specificity of detection can be higher than those obtained according to the conventional indirect method, and the rapid detection of brucellosis is realized; the problems of high interference, and high false positive result or false negative result occurrence rate caused by long detection period, poor specificity and low sensitivity in the conventional brucellosis detection process are solved.

The invention discloses a method for detecting hantaan virus strain genome. The method has the following steps: (1) two pairs of hantaan virus general primers are designed; (2) the hantaan virus detection method comprises the following steps that: first, viral specific RNA extraction kit is utilized to extract the total RNA in a serum sample; second, a nucleic acid analyzer is adopted to detect the RNA content, and the RNA is taken to perform the RT-PCR reaction; third, RT-PCR:RT uses HV group-specific primers such as P0, PCR uses HV general primers such as P1P2 and P3P4; fourth, viral nucleic acid amplified results are observed through agarose gel electrophoresis, and a specific nucleic acid band appears. The method is intuitionistic and predominant, the sensibility is good, and the effects are good; the serum detection rate in recent ten years is 79.2 percent, and the serum detection rate in twenty years ago can still reach to 70.5 percent; the method is applied to the laboratory detection of hantaan virus infection and the molecular epidemiological survey.

The related detection method for bacillus tubercle specific secretory antigen comprises: culturing or using bio-engineering method to obtain ESAT-6, MPT53, MPT64, MPT63 and MPT70, preparing opposite antigen, and realizing synchronous detection for five antigens. This invention improves detection sensitivity and can provide effective evaluation for diagnosis.

The invention discloses a liquid-based cell sheet producing system which comprises a rack, a sheet producing platform fixed on the rack, a plurality of sheet producing plates fixed on the sheet producing platform, a moving device perpendicularly connected with a bearing frame of the rack, a mechanical arm perpendicularly and slidingly connected with the moving device, a cantilever perpendicularly and slidingly connected with the mechanical arm, a reagent bottle connected with the cantilever by a catheter, and a microcomputer system controlling the moving device, the mechanical arm and the cantilever, wherein a control window is arranged on the rack, and controls the microcomputer system; and the reagent bottle comprises a waste liquid barrel, a flushing fluid reagent bottle part and a buffer fluid reagent bottle part. A liquid-based cell sheet producing method adopts the liquid-based cell sheet producing system. With the adoption of the technical scheme, the defects that a system operation mode is single, a procedure is solidified, a pipeline cannot be flushed automatically, a requirement on the quantity of specimens is strict, flux is low, energy consumption and reagent consumption material loss are great, a failure rate is high, maintenance is difficult, and equipment is costly are overcome.

The invention discloses an accurate screening system for a cervical cancer. The system comprises a digital electronic colposcope, an expander, an intelligent terminal and a cervical disease managementplatform; the digital electronic colposcope can be fixedly arranged at the rear end of the expander and is used for observing a cervix uteri and transmitting an observed cervix uteri image to the intelligent terminal through a network; the intelligent terminal is provided with a colposcope examination APP, and is used for receiving and displaying the cervix uteri image observed by the digital electronic colposcope and guiding accurate sampling of the cervix uteri under a visual condition, and the cervix uteri image is further uploaded to the cervix uteri disease management platform; and the cervical disease management platform comprises a cervical disease cloud database and is used for remotely sharing screened cervical images by medical experts. According to the accurate screening systemfor the cervical cancer, the accuracy of the accurate screening of the cervical cancer is remarkably improved, and meanwhile comprehensive management of screening, diagnosis and follow-up visit of female cervical diseases is achieved.

The invention provides a hydrothorax and ascite malignant cell embedding method. The method comprises the following steps: putting hydrothorax and ascite in a plurality of 50-ml centrifugal tubes respectively, adding analytically pure ethyl alcohol into the centrifugal tubes respectively, and uniformly mixing and standing for 2-4h to obtain sediments; sucking up moisture in the sediment, wrapping a specimen by using a fixing mold and putting into a material drawing box; dehydrating the obtained specimen with the ethyl alcohol, and transparentizing the dehydrated specimen by adopting a transparent agent-xylene; putting the transparentized specimen in a 70-DEG C constant-temperature box for wax dipping, wherein an embedding agent is paraffin wax; and embedding the specimen after wax dipping with paraffin in an embedding workstation, pouring the specimen and the paraffin into an embedding box, putting the embedding box into cold water to rapidly solidify the paraffin after the paraffin on the surface of the embedding box is solidified, taking the paraffin block out of the embedding box and storing. The method can greatly increase the positive rate of cast-off cells, and can be used in further diagnosis of molecules, genes, immunohistochemistry and the like.

The invention discloses a breast cancer-related kinase mutation detection panel. The detection panel comprises receptor tyrosine kinase (RTK), phosphatidylinositol 3-kinase (PI3K), mitogen-activated protein kinase (MAPK) related genes, and an exon region and a part of intronic regions of a downstream gene. The invention further discloses a kit containing the detection panel. The detection panel can detect gene mutation guiding diagnosis and treatment more efficiently, is of great significance to clinical diagnosis and discovery of a target spot of target treatment, and can help lower gene detection cost of patients.

The invention discloses a network malicious image detection method based on a visual word bag. The method is specifically implemented according to the following steps of: image pretreatment, including obtaining a skin color area in the image; feature extraction and description, including obtaining feature vectors of the key point in the skin color area; building a visual word bag, screening out the feature vector which can best represent the feature of the image from the image key point, and thereby constituting the visual word bag of the image; image detection, including classifying the visual word bag of the image by a trained classifier, and thereby completing the detection of the malicious image. When being used for identifying and detecting malicious images, compared with other similar method, the network malicious image detection method based on the visual word bag is higher in correct detection rate and shorter in detection time, can be used as an efficient network malicious image detection method, and has certain theoretical and practical values.

The invention discloses a DNA methylation qPCR kit for cervical cancer detection as well as a use method and an application thereof. The kit is used for screening and diagnosing cervical cancer by detecting or measuring the methylation state or level of one or more specific genes in DNA of a tested sample. Through experimental tests, the method has the characteristics of quickness, high sensitivity, high specificity and the like, and can be used for diagnosing the human cervical cancer in time in the early stage, so that the early accurate diagnosis of the cervical cancer becomes possible, andthe waste of medical resources is avoided. The kit provided by the invention can significantly improve the positive detection rate and specificity (real negative rate) of early cervical cancer.

This invention is a method of preparing and detecting diagnostic chip of human papillomavirus gene. On the base of the chip there are 16 kinds of stylet which arrange in array, they consist of one general stylet, 15stylet subjected to HPV. There are 6, 11, 16, 18, 31, 33, 35, 39, 42, 43, 44, 45, 52, 56, 58 and so on 15 in total. We add a nucleic acid to the end of the atyle, irradiate the chip under the ultraviolet radiation for 5minute, fix the aryle to the surface of the chip, and construct to be diagnostic chip. PCR primer adapts 2-24 chain of primer. This invention has the virtue of quick, exactness, simple and convenient, special recognition, high information, it is prone to expand, and has high economic and social efficiency on clinic application.

The invention discloses a kit for detecting mycobacterium tuberculosis based on blood free nucleic acid. The kit comprises PCR (polymerase chain reaction) solution, a primer, an enzyme and an interiorlabel, the primer includes a primer for detecting MTB target nucleotide and a primer for detecting the interior label, and a nucleotide sequence of the primer for detecting the MTB target nucleotideis as shown in SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3 and SEQ ID NO.4. A detection method of the kit includes a PCR-fluorescent probe method and a PCR-membrane chromatography hybridization method. According to the kit, high-sensitivity detection based on blood free nucleic acid can be achieved, the positive rate of tubercular patients with negative sputum smears, extra pulmonary tuberculosis patients and HIV (human immunodeficiency virus) concurrent tuberculosis infection patients is remarkably increased, the screening accuracy of tuberculosis infection patients is improved, transmission routescan be effectively controlled by detecting blood samples, safety risks of medical staff and inspection staff are reduced, and the kit has ground-breaking clinical values to guiding pharmacy of tuberculosis and control and prevention of diseases.