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Method for detecting immunological turbidimetry of hypersensitive cardiac troponin I magnetic microspheres and detection kit

A technology of cardiac troponin and detection kits, which is applied in biological testing, measuring devices, material inspection products, etc., can solve the problems of low analytical sensitivity, achieve the effects of removing free antibodies, prolonging shelf life, and improving detection sensitivity

Inactive Publication Date: 2017-12-01
北京科跃中楷生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current product has the defect of low analytical sensitivity

Method used

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  • Method for detecting immunological turbidimetry of hypersensitive cardiac troponin I magnetic microspheres and detection kit
  • Method for detecting immunological turbidimetry of hypersensitive cardiac troponin I magnetic microspheres and detection kit
  • Method for detecting immunological turbidimetry of hypersensitive cardiac troponin I magnetic microspheres and detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1 Preparation of Cardiac Troponin I Quantitative Detection Kit

[0044] Specific steps are as follows:

[0045] ⑴ Preparation of reaction diluent reagent: take 800mL 25mM pH7.4 phosphate buffer solution, add 5g of bovine serum albumin, 20ml of polyethylene glycol 8000, 1ml of Tween 20, 1ml of proclin300, mix well and dissolve, then filter with 0.22um microporous Membrane filtration, finally dilute to 1000ml with corresponding buffer solution, seal and store for later use.

[0046] (2) Preparation of matrix solution for magnetic microspheres coupled with cTnI antibody: take 800mL 25mM pH7.4 phosphate buffer solution, add 5g of bovine serum albumin, 5g of casein, 100g of sucrose, 50g of polysucrose-400, 1ml of Tween-20 ,Triton X-1001ml, proclin300 1ml are fully mixed and dissolved, then filtered with a 0.22um microporous membrane, and finally adjusted to 1000ml with the corresponding buffer solution, that is, the prepared matrix solution, sealed and stored for la...

Embodiment 2

[0054] Example 2. Preparation of Cardiac Troponin I Quantitative Detection Kit

[0055] ⑴Preparation of reaction diluent reagent: take 800mL 50mM pH7.4 phosphate buffer solution, add bovine serum albumin 20g, polyethylene glycol 8000 50ml, Tween 20 3mL, proclin300 5mL, mix well and dissolve, then use 0.22um microporous filter Membrane filtration, finally dilute to 1000mL with corresponding buffer solution, seal and store for later use.

[0056] ⑵Preparation of matrix solution of magnetic microspheres coupled with cTnI antibody: take 800mL 50mM pH7.4 phosphate buffer solution, add bovine serum albumin 20g, casein 20g, sucrose 200g, polysucrose-400 200g, Tween-20 10mL ,Triton X-100 10mL, proclin300 5mL are fully mixed and dissolved, then filtered with a 0.45um microporous membrane, and finally adjusted to 1000mL with the corresponding buffer solution, that is, the prepared matrix solution, sealed and stored for later use.

[0057] All the other steps are the same as in Example ...

Embodiment 3

[0058] Example 3. Preparation of Cardiac Troponin I Quantitative Detection Kit

[0059] ⑴ Preparation of reaction diluent reagent: take 800mL 10mM pH7.4 phosphate buffer solution, add bovine serum albumin 0.5g, polyethylene glycol 8000 2mL, Tween 20 0.1mL, proclin300 0.5mL, mix well and dissolve with 0.22um Filter with a microporous membrane, and finally dilute to 1000mL with the corresponding buffer solution, and keep it sealed for future use.

[0060] ⑵Preparation of matrix solution of magnetic microspheres coupled with cTnI antibody: take 800mL 10mM pH7.4 phosphate buffer solution, add bovine serum albumin 0.5g, casein 0.5g, sucrose 2g, polysucrose-400 2g, Tween- 20 0.1mL, Triton X-1000.1mL, proclin300 1mL, mix well and dissolve, then filter with 0.22um microporous membrane, and finally dilute to 1000mL with corresponding buffer solution, that is, the prepared matrix solution, sealed and stored for later use .

[0061] All the other steps are the same as in Example 1.

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Abstract

The invention relates to the technical field of clinic kits and particularly relates to a method for detecting immunological turbidimetry of hypersensitive cardiac troponin I magnetic microspheres and a detection kit. The kit comprises a reaction diluent reagent, a cTnI antibody-coupled magnetic microsphere reagent and a cTnI correction product. Compared with a detection result of an existing cTnI chemiluminiscence detection kit, the method and the detection kit have the advantages that by carrying out statistic analysis, the relativity is good, no remarkable difference exists, the kit can be clinically popularized and used, and a relatively good choice is provided for the detection of cardiac troponin I.

Description

technical field [0001] The invention relates to the field of detection of clinical kits, in particular to an ultrasensitive cardiac troponin I magnetic microsphere immunoturbidimetric detection method and a detection kit. Background technique [0002] Cardiac troponin I (cTnI) is one of the subunits of the cardiac troponin complex. cTnI has high sensitivity and good tissue specificity, and has been widely accepted clinically. It has not only become the "gold standard" for the diagnosis of acute myocardial infarction, Moreover, it has become the most suitable marker for monitoring the condition of myocardial disease, observing the curative effect and evaluating the prognosis. [0003] At present, the detection methods of cTnI mainly include enzyme-linked immunosorbent assay, chemiluminescence method, colloidal gold immunochromatography, gold standard silver staining method, latex-enhanced immunoturbidity and other related methods. Latex-enhanced immune turbidimetry is easy t...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/577G01N33/543
CPCG01N33/54326G01N33/54346G01N33/577G01N33/6893G01N2333/4712G01N2800/32G01N2800/324
Inventor 柳静
Owner 北京科跃中楷生物技术有限公司
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