98 results about "Adult worm" patented technology

The invention discloses a brown planthopper survival and growth development-associated NlPIK3R1 gene, and a coded protein and an application thereof. The gene NlPIK3R1 for coding a brown planthopper phosphatidyl inositol 3 kinase p85 alpha subunit comprises a nucleotide sequence as shown in SEQ ID NO:1 and a homologous mutant sequence thereof. The gene has an important function in a survival and growth development process of the brown planthopper, and is restrained in function to cause brown planthopper survival rate drop, slow growth and delayed adult eclosion. The protein coded by the brown planthopper NlPIK3R1 gene comprises an amino acid sequence as shown in SEQ ID NO:2 and a homologous mutant sequence thereof. The gene is restrained in function to cause brown planthopper survival rate drop, slow growth and delayed adult eclosion. RNA interference of the brown planthopper NlPIK3R1 gene is applied to control brown planthopper. RNA interference is successfully performed on the gene, the survival rate of pests is decreased according to the results, the survival and the growth development are obviously restrained, the weight is reduced, and the adult eclosion is delayed.

The invention discloses a Wnt family gene which is amplified for the first time from Schistosoma japonicum 19-day schistosomulum by utilization of RACE technology, wherein, sequence analysis indicates that: a complete coding frame of the gene comprises 1311bp, 436 amino acids coded; and theoretical molecular weight of 49.6kD. Homology analysis results indicate that: amino acid sequences of the gene have typical Wnt family protein characteristics; similarity of the amino acid sequences with amino acid sequences of Dugesia japonica and human Wnt4 is relatively 43 percent and 37 percent; the gene is presumed to be a Wnt4 gene of schistosome and named as Sjwnt4 (GenBanK Log-On No.: DQ643829). Real-time quantitative PCR analysis indicates that: the gene is expressed all in 14-day schistosomulum, 19-day schistosomulum, 31-day imago, 44-day female worms and 44-day male worms. The invention constructs a pronucleus expression vector pGEX-4T-2-Sjwnt4 of the gene and applies an Escherichia colisystem for expression; expression proteins exist in the form of inclusion bodies; Western blottings indicate that expression products can be identified by crude antigen immune serums of Schistosoma japonicum imago.