95 results about "Adult worm" patented technology

The invention discloses a method for artificially raising plant bug parasitic wasps, which belongs to an artificial raising technology of natural enemy insects. In the method, Peristenus spretus is successfully bred by the following steps of: the inoculation of the Peristenus spretus, the raising of parasitized nymphae of green plant bugs, the pupation of parasitic wasps, the raising of adult worms and the like. The method is simple and easy, the occupied space is small, and the raising cost is low. By the method, the Peristenus spretus can be bred on a large scale to realize the uninterrupted breeding of the Peristenus spretus all the year around, so that the need of experimental study can be met, and a large number of insect sources can be provided for serious occurrence areas of plant bugs so as to reduce the harm of the plant bugs to crops such as cotton and the like.

The invention relates to a method for raising an experimental population of Rhynchophorus ferrugineus. The method comprises the following steps of: performing eclosion on pupae of Rhynchophorus ferrugineus to be raised into worms, then putting each pair of adult worms which are taken as a group into a container with hydromel for mating and oviposition, routinely disinfecting eggs and then hatching to obtain larvae, inoculating the larvae into a Rhynchophorus ferrugineus larvae raising container loaded with a semi-artificial feed of the Rhynchophorus ferrugineus larvae, and putting into a healthy worm raising room for raising until the larvae are pupated. The method is simple, the life history of the Rhynchophorus ferrugineus is finished indoors by adopting the semi-artificial feed, and a large quantity of intact eggs, accurate larvae at various ages and pupae are obtained under the condition of not hurting the eggs and the larvae; and the method has the characteristics of short development duration, high survival rate and the like, effectively and greatly shortens the generation cycle, and provides adequate experiment populations for completing the deep and systemic research on the Rhynchophorus ferrugineus in the fields such as biology, ecology and control technologies and the like.

The invention relates to an anti-echinococcosis antigen monoclonal antibody hybridoma cell strain, an anti-echinococcosis antigen monoclonal antibody and application of the anti-echinococcosis antigenmonoclonal antibody. The anti-echinococcosis antigen monoclonal antibody hybridoma cell strain is classified and named as a hybridoma cell strain XJ10D8D10. The strain is preserved in the China Center for Type Culture Collection (CCTCC) on September 25th, 2017. The preservation address is Wuhan University, Wuhan, China, and the preservation number is CCTCC NO: C201789. According to the invention,an echinococcosis adult surface membrane antigen is extracted; a Balb/c mouse is immunized; splenic lymphocytes secreting anti-echinococcosis adult surface membrane antigen are obtained; the hybridoma cell strain secreting the anti-echinococcosis adult surface film antigen is obtained by fusing the splenic lymphocytes with SP2/0 cells, so that an echinococcosis-resistant adult surface film antigen antibody is obtained, and the echinococcus granulosus infection of dogs can be sensitively detected through fecal antigen detecting by using the antibody as a diagnostic reagent. And necessary conditions are provided for preparation of large-scale general survey detecting kits.

The invention discloses an integrated method for biologically preventing grape leafhoppers. The method comprises the following steps: adopting yellow plates to lure and kill wintering adult worms after a grape pier is dug; hanging the yellow plates in parallel to a first iron wire or a second iron wire of a trellis surface close to the root, wherein 300-400 yellow plates are used in each hectare;preventing first-generation nymphs when the population density reaches 12/100 mesh; preventing the grape leafhoppers by adopting medicaments, i.e. 1,500 times of 2.5 percent of success or chlorbenzuron; remaining vegetative shoots of small fruit ears below 500g according to variety characteristics before and after flowering; remaining 1-2 vegetative shoots of each larger fruit ear over 500g to keep favorable ventilation and photopermeability of the trellis surface and lessen spotted leafhopper hazards; applying decomposed organic fertilizer with the concentration of 2-3 tons/667m<2> in autumn;and applying the fertilizer for 2-3 times in the whole growth period to improve the plant resistance. The invention provides an integrated biological prevention method for comprehensively and biologically preventing and controlling the grape leafhoppers below an economic threshold value.

The invention relates to the technical field of artificial cultivation of edible (medicinal) fungus, and particularly relates to artificial cultivation of an egg-sprouted worm grass. The artificial cultivation of the egg-sprouted worm grass is characterized in that fresh eggs (except for the fresh eggs adopted in the prior art), incubated eggs, seedlings incubated by the eggs, seedlings and adults of varieties easy to get in ovipara, such as a tortoise, a soft-shelled turtle, a snake, a quail, a pigeon, a chicken, a duck and a goose, and adults of a fish, a shrimp, a loach and an eel are taken as a culture medium, so that various distinctive egg, seedling and adult worm grass, which are abbreviated as the egg-sprouted worm grass, can be cultured by inoculating a strain of the worm grass. Therefore, the varieties of the worm grass can be increased, so that the market demand can be satisfied.

The invention relates to a composition capable of trapping and killing onionmaggot adult worms, which is prepared from Clothianidin, honey, sodium benzoate and water, as well as a method for utilizing a Clothianidin-containing composition capable of trapping and killing onionmaggot adult worms in preventing and curing onionmaggot of garlic, shallot and onion. In the invention, it is discovered that the composition capable of trapping and killing onionmaggot adult worms, which is prepared by Clothianidin, honey, sodium benzoate and water can trap and kill onionmaggot adult worms massively, reduce the egg laying amount of adult worms and effectively control the onionmaggot harm when being contained in containers or sprayed on blue insect sticky boards and then placed in the garlic, the shallot and the onion fields. The composition can be widely used in preventing and curing various leaf miners for vegetables, and has large application value and remarkable economic and social benefits.

The invention provides a Schistosoma japonicum gene recombination living vector vaccine which is composed of porcine pseudorabies and a schistosoma japonicum antigen gene; the schistosoma japonicum antigen gene comprises Sj26GST, SjFABP and Sj23 to form monovalent recombination living vector vaccines rPRV/Sj26GST, rPRV/FABP and rPRV/Sj23 as well as multivalent living vector vaccines rPRV/Sj26GST-FABP and rPRV/Sj26GST-FABP-Sj23; the imago reduction rate is between 38.4 and 56.5 percent; the liver egg reduction rate is between 38.8 and 70.6 percent; the vaccine can effectively induce the immunity protection force for resisting the schistosoma japonicum, can generate longer immunity protection effect for one time immunity, does not have pollution or harmful effect to environment and does not cause untoward effect.

The invention belongs to the field of agricultural biotechnology, and in particular, relates to a specific SS-COII primer of cydia pomonella l. and an application thereof. The specific primer can only have the amplification ability to the cydia pomonella l., the size of the amplified product is 262 bp, and the specific primer has good detection effect on single eggs and adult residual bodies. The primer improves the accuracy of detection of the cydia pomonella l., saves the detection time, and is suitable for promotion in import plant quarantine, domestic plant quarantine and field control in the form of kit.

The invention relates to a group of bemisia tabaci prevention and control target genes and combined application thereof. The group of bemisia tabaci prevention and control target genes comprise a BtGlu gene as shown in SEQ ID NO.1, a BtArgH gene as shown in SEQ ID NO.2 and a BtTY gene as shown in SEQ ID NO.3. The research thought of taking a sugar transporter gene, a basic group and a amino acid synthetic gene as the bemisia tabaci prevention and control target genes is provided for the first time; and experiments of the survival rate of the adult bemisia tabaci, the egg laying rate of the female bemisia tabaci, the influence on symbiotic bacteria of the female bemisia tabaci, the influence on the virus acquiring capacity of the female bemisia tabaci TYLCV and the like verify that the silent expression can obtain a short-term prevention and control effect-reduction of the survival rate of the bemisia tabaci and a long-term prevention and control effect-reduction of the reproductive capacity of the bemisia tabaci.

The invention provides a Dirofilaria immitis enzyme-linked immunosorbent assay (ELISA) kit for a dog, and a preparation method of the Dirofilaria immitis ELISA kit. Homologous genes are named methyl tri-fluoro pyruvate (MTFP) with homology of 88 percent, screened from a Dirofilaria immitis adult complementary deoxyribose nucleic acid (cDNA) expression library and used for encoding Brugia malayi myosin tail group proteins, MTFP proteins which are expressed in escherichia coli (E.coli) BL21(DE3) serve as antigens, and an indirect ELISA method for detecting the Dirofilaria immitis infection of the dog is established. The kit is high in specificity, high in sensitivity, high in accuracy and high in stability, has the advantages of simplicity in operation, quickness in detection, convenience for judgment and the like, and is suitable for clinical rapid diagnosis and large-scale application to epidemiological investigation in grassroots units, villages and the like.

The present application relates to improved recombinant protein production processes through the use of novel fusion tags. In particular, the present application discloses novel fused proteins comprising an aminoacidic sequence that corresponds to a fragment H or an aminoacidic sequence that corresponds to a calcium-binding protein excreted/secreted by the adult worm of Fasciola hepatisa, namely, Fh8 and Fh22 proteins, followed by an aminoacidic sequence that corresponds to a non-related protein fragment or protein, and methods for its preparation and its use thereof.

A preparation method of schistosome antigen marked gold nanorods comprises the following steps: utilizing a reductant to reduce a HAuCl4 solution to prepare spherical gold nano-particle seeds; addingthe spherical gold nano-particle seeds into a growth medium, and enabling the spherical gold nano-particle seeds to grow into rod-like gold nanorods in a rod micelle solution; and marking the gold nanorods with a schistosome adult worm antigen and/or a schistosome cercaria antigen to obtain the schistosome antigen marked gold nanorods. As the gold nanorods are synthesized by a 'seed mediated growth method', the productivity and the gold particle conversion rate of products are superior to those of products prepared by a template method in which nucleation and growth are synchronously performed; and the lengths and the widths of the gold nanorods are obviously superior to spheres. The preparation method has more sensitive and quick results than ELISA, a sensitive and quick serological diagnostic technique for detecting schistosomiasis is established, and a simple, convenient and reliable clinical inspection technique is provided for serological diagnosis of early or light schistosomiasis infection.

The invention provides a method for detecting developmental toxicity by using caenorhabditis elegans, which belongs to the field of ecological risk evaluation, according to the invention, the method comprises the following steps: using a 24-hole cell culture plate as a main exposure environment, preparing an NGM culture medium with a specific gradient, exposing a test substance to the caenorhabditis elegans eggs on one side of the culture medium, using the sensitivity of caenorhabditis elegans to the external environment change in the development stage from eggs to adults, using escherichia coli food for inducing nematodes to generate movement behaviors, detecting the developmental toxicity according to the characteristics of development and movement, realizing direct observation even without assistance of a microscope, and quickly and qualitatively judging the development toxicity of the tested substance by directly observing the existence and quantity of the food side nematodes withnaked eyes. The time from exposure to index detection is about 24 hours, the index detection time is shortened, naked eye observation and microscopic observation are integrated, and the detection result has high accuracy and reliability.

The invention relates to an immunochromatographic test strip for rapid detection of ancylostomiasis infection. The test strip includes: a sample pad, a gold labeled pad that is closely connected to the sample pad and contains a colloidal gold labeled probe, a cellulose membrane closely connected to the gold labeled pad and a water absorption pad closely connected to the other end of the cellulose membrane. One cellulose membrane end far from the gold labeled pad is provided with a quality control line, the cellulose membrane between the quality control line and the gold labeled pad is equipped with a detection line, which is composed of a hookworm purification worm antigen, and the hookworm purification adult worm antigen is a soluble antigen solution of Necator Americanus adult worms. The colloidal gold labeled probe is staphylococcus aureus A protein or s streptococcus G protein. The control line is composed of the antibody specifically binding with the colloidal gold labeled probe. The invention also provides a preparation method of the immunochromatographic test strip. The immunochromatographic test strip provided by the invention has the advantages of simplicity, sensitivity, specificity and rapidity, and can be applied to clinical and field use of human, livestock and wild animals at the same time.

The invention provides a bradtsua idiruogaga trapping and killing agent, a preparation method and an application thereof. The trapping and killing agent includes a trapping agent and a killing agent. The bradtsua idiruogaga trapping and killing agent is simple in preparation method, employs easy-to-obtain raw materials, has stable performance and is convenient to use. Compared with chemical prevention and treatment on the bradtsua idiruogaga in the prior art, the trapping and killing agent, through addition of trace amount of the trapping agent, can effectively control damage from the bradtsua idiruogaga and reduces use time and amount of pesticide, thereby effectively solving the problems of resistance, pesticide residue and environment pollution due to chemical prevention and treatment. The trapping and killing agent can trap and kill adult worms of the bradtsua idiruogaga high-effectively, has a long lasting time, can directly kill the adult worms or deprive normal oviposition capability of the adult worms, thereby greatly reduces the population number of the bradtsua idiruogaga. The trapping and killing agent provides reliable substance guarantee for green prevention and control on leek.

The invention discloses cockroach glycoprotein as well as a preparation method and application thereof. The preparation method comprises the following steps: raw material treatment: killing cockroach adults, screening out impurities in the adults, cleaning the cockroaches with warm water, drying to obtain medicinal materials, and crushing for later use; performing extraction of cockroach glycoprotein; and purifying the cockroach glycoprotein. According to the invention, a glycoprotein substance for regulating immunocompetence and antioxidant activity is extracted from cockroaches, and the glycoprotein substance is an animal-derived natural immunomodulator and antioxidant and can be applied to treatment of immunosuppression, immune disorder and chronic diseases related to lipid peroxidation.

The invention discloses a primer group capable of simultaneously detecting paragonimus westermani, paragonimus skriabini and paragonimus heterotremus, a kit and a method. The kit comprises a test paper strip, a paragonimus DNA positive control solution and a detection solution, wherein the detection solution specifically comprises an LAMP buffer solution, the primer group, a Bst 2.0 DNA polymerase, dNTP, MgSO4 and ddH2O, and the primer group comprises an outer primer F3, an outer primer B3, an inner primer FIP, an inner primer BIP and a probe Probe. The kit can be used for rapidly detecting genomic DNA of paragonimus in a sample. The invention provides the method for detecting the genomic DNA of the paragonimus. The method can be used for detecting genomic DNA of adults, encysted cercariaand ova of the paragonimus. The kit is high in sensitivity, high in specificity, relatively good in stability and simple in operation, can better meet the requirements of people of different hierarchies and facilitates popularization to grass roots.

The invention discloses a Wnt family gene which is amplified for the first time from Schistosoma japonicum 19-day schistosomulum by utilization of RACE technology, wherein, sequence analysis indicates that: a complete coding frame of the gene comprises 1311bp, 436 amino acids coded; and theoretical molecular weight of 49.6kD. Homology analysis results indicate that: amino acid sequences of the gene have typical Wnt family protein characteristics; similarity of the amino acid sequences with amino acid sequences of Dugesia japonica and human Wnt4 is relatively 43 percent and 37 percent; the gene is presumed to be a Wnt4 gene of schistosome and named as Sjwnt4 (GenBanK Log-On No.: DQ643829). Real-time quantitative PCR analysis indicates that: the gene is expressed all in 14-day schistosomulum, 19-day schistosomulum, 31-day imago, 44-day female worms and 44-day male worms. The invention constructs a pronucleus expression vector pGEX-4T-2-Sjwnt4 of the gene and applies an Escherichia coli system for expression; expression proteins exist in the form of inclusion bodies; Western blottings indicate that expression products can be identified by crude antigen immune serums of Schistosoma japonicum imago.

The invention relates to an artificial feed for breeding adult worms of ladybug sexmaculates. The artificial feed is composed of yeast powder, sucrose, pig liver, vitamins, cholesterol, honey, olive oil, oatmeal, eggs and pollen. The product can be prepared in the form of granules, powder and agar. Among them, the feed in a powdered state has the best effect, and the mass ratio is: 50-60:50-60:200:0.1-0.3:0.5-0.7:80-100:3-4 :50-70:50-70:30-50. The feed formula can satisfy the normal life activities of the adult worms of the ladybug sexmaculates, and the biological characteristics and the predation effect are not different compared with the natural prey aphid of the ladybug. The raw materials of the feed of the invention are easy to obtain, the product is convenient to prepare, easy to operate, low in cost, and is beneficial to factory production, and provides a technical and material basis for large-scale reproduction and application of ladybugs.

The present invention discloses Schistosoma japonicum specific female fluke protein and its gene sequence and specifically combined antibody as well as Schistosoma japonicum preventing and treating vaccine prepared with the specific female fluke protein as immunogen and the application of the specific female fluke protein in preparing medicine for preventing and treating Schistosoma japonicum. Applying the specific female fluke protein can inhibit the ovipositiom of female fluke to treat schistosomiasis and prevent Schistosoma japonicum diffusion.