Schistosome antigen marking gold nanorods and preparation method thereof

Abstract

A preparation method of schistosome antigen marked gold nanorods comprises the following steps: utilizing a reductant to reduce a HAuCl4 solution to prepare spherical gold nano-particle seeds; addingthe spherical gold nano-particle seeds into a growth medium, and enabling the spherical gold nano-particle seeds to grow into rod-like gold nanorods in a rod micelle solution; and marking the gold nanorods with a schistosome adult worm antigen and / or a schistosome cercaria antigen to obtain the schistosome antigen marked gold nanorods. As the gold nanorods are synthesized by a 'seed mediated growth method', the productivity and the gold particle conversion rate of products are superior to those of products prepared by a template method in which nucleation and growth are synchronously performed; and the lengths and the widths of the gold nanorods are obviously superior to spheres. The preparation method has more sensitive and quick results than ELISA, a sensitive and quick serological diagnostic technique for detecting schistosomiasis is established, and a simple, convenient and reliable clinical inspection technique is provided for serological diagnosis of early or light schistosomiasis infection.

Description

technical field [0001] The invention belongs to the field of biological detection, and in particular relates to a schistosome antigen-labeled nano-gold rod and a preparation method thereof. Background technique [0002] Nanotechnology is a multidisciplinary science and technology that uses single atoms and molecules to create substances. The interval between the microscopic world represented by atoms and molecules and macroscopic objects between 1-100nm is the main research area of ​​nanotechnology. Nanoparticles represented by noble metals have special and excellent physical and chemical properties, so that they have received great attention in the fields of biosensing, biotherapy, and modernization of traditional Chinese medicine, and have become a research hotspot for scholars. [0003] As a typical noble metal, gold (Au) has a special size dependence, which makes it show great advantages and development prospects in medical inspection, diagnosis and treatment. The main...

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Problems solved by technology

The main problems are as follows: (1) The detection method for circulating antigen is a commonly used method for detecting acute infection of schistosomiasis, but the main disadvantage of this method is that due to the low content of circulating antigen in the early stage of schistosomiasis infection, it cannot be detected Sensitivity, high misdiagnosis rate

(2) The ability to distinguish between current infection and past infection is poor. At present, most schistosomiasis detection systems for schistosomiasis cannot achieve satisfactory results. The presence of antibodies in the patient's body can still be detected for 3 years

(3) The optimal period for the detection of schistosomiasis antibodies is not the same, and each patient has its own regularity. Using a single traditional method for detection may miss the detection of patients with pre-infection or late infection, and may also cause false positives

Low-grade infection is the main epidemiological feature of schistosomiasis, but the sensitivity of existing immunological detection methods is not high, which is a key reason for the high rate of misdiagnosis and misdiagnosis of schistosomiasis; schistosomiasis has become a difficult clinical disease , which affects the cure rate of patients, so it is necessary to have a highly sensitive schistosomiasis detection and diagnosis technology clinically

[0011] The physical and chemical characteristics of AuNPs make it possible to be widely used in the field of medical testing, but its application to the diagnosis of schistosomiasis or other parasitic diseases has not been reported

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