Lyceum barbarum polysaccharide-containing protective liquid and application method thereof for enhancing adaption of mesenchymal stem cells to in vivo pathologic microenvironment

Abstract

Disclosed is a lyceum barbarum polysaccharide-containing protective liquid and an application method thereof for enhancing adaption of mesenchymal stem cells to in vivo pathologic microenvironment. The method comprises the steps of separation, culture and identification of the mesenchymal stem cells, application function evaluation and composition of the lyceum barbarum polysaccharide-containing protective liquid, mechanism of action of the lyceum barbarum polysaccharide-containing protective liquid and the like. The natural herb extract of lyceum barbarum polysaccharides serve as the major component to replace existing methods such as gene editing and chemical treatment, namely, the purely natural herb extract is applied to intervening the mesenchymal stem cells to enable the mesenchymalstem cells to better adapt to the complex in vivo pathologic microenvironment and to improve the survival ability and the therapeutic potential. The lyceum barbarum polysaccharide-containing protective liquid can significantly improve the survival rate of the mesenchymal stem sells in the in vivo pathologic microenvironment.

Description

technical field [0001] The invention relates to a new application of wolfberry extract in the field of biomedicine, more specifically, to a protective solution containing wolfberry polysaccharide and its application method in improving mesenchymal stem cells to cope with complex pathological microenvironments in vivo. Background technique [0002] Mesenchymal stem cells (MSCs) are a kind of multipotent precursor cells derived from the mesoderm in early embryonic development, which have the potential of self-renewal and differentiation to all cell types of the three germ layers. Based on self-renewal, multi-directional differentiation potential and immune regulation function, MSCs have incomparable therapeutic advantages in tissue repair and immune regulation compared with traditional treatment methods. Clinical studies have confirmed that MSCs have shown subversive therapeutic effects in the treatment of many diseases including diabetes, cardiovascular diseases, autoimmune d...

AI Technical Summary

Problems solved by technology

However, the existing technical methods, on the one hand, have carried out gene editing or chemical drug intervention on the cells, which will affect the normal physiological and metabolic activities of the cells to varying degrees, and may change the original biological characteristics of the cells and the possibility of malignant transformation into tumor cells. Potential, there are unknown safety issues, which seriously affect the clinical transformation of mesenchymal stem cells; on the other hand, there are limitations in the intervention effect, that is, only for one pathological microenvironment in vivo, and it is impossible to give mesenchymal stem cells a comprehensive response to complex in vivo conditions. The capacity of the pathological microenvironment

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