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Artificial liver bioreactor

A bioreactor, artificial liver technology, applied in suction devices and other directions, can solve the problems of low hepatocyte density, uneven artificial liver function, and flow away distribution of hepatocytes, and achieves favorable microenvironment for cell adhesion and good microenvironment. , the effect of increasing the density of hepatocytes

Inactive Publication Date: 2013-05-15
SUN YAT SEN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there are many problems in cell culture in artificial liver bioreactors, such as the density of hepatocytes cultured in the bioreactor is too low to reach the 2 ~ 3×1010cells / m2, hepatocytes are easily detached from the culture medium, which causes hepatocytes to flow away with the circulating culture medium and distribute in the bioreactor Inhomogeneous artificial liver function decline, and hepatocyte death caused by insufficient oxygen supply in the culture medium

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0034] Example 1: Chitosan and galactosylated chitosan materials were used as raw materials for nanofibrous scaffolds to make this novel bioreactor.

[0035]The specific preparation method of the nanofibrous scaffold: dissolving chitosan and galactosylated chitosan in 70% formic acid solution with a concentration of 3-5wt%. Add 10wt% (PEO / galactosylated chitosan) PEO to the above solution under stirring condition, dissolve for 3 hours and centrifuge to remove the bubbles in the solution. The solution was then filled into a 5 ml glass syringe with a 6 gauge needle. Push the solution at a speed of 1ml / h under the impetus of a micro-syringe pump. A voltage of 15 kV was applied across the needle and the collecting plate, and the distance between the collector and the needle was 15 cm. Finally, the electrospun fibers were collected on a built-in plate in a bioreactor, and then dried under vacuum at 40°C for two days to remove residual formic acid.

Embodiment 2

[0036] Embodiment 2: use PLGA as the raw material of nanofiber support to make this novel bioreactor

[0037] The specific preparation method of the nanofiber scaffold: PLGA was dissolved in chloroform to prepare a solution with a concentration of 21.5%, and then the solution was filled into a disposable syringe equipped with a No. 6 needle. A DC voltage of 10-15 kV was applied between the collecting plate and the needle, and the solution was pushed out from the syringe at a speed of 1 ml / h by a micro syringe pump. The distance between the needle and the pedal is 10-15cm. When the spinning was completed, the built-in flat plate covered with PLGA electrospun fibers was removed and placed in a vacuum desiccator for two days to remove residual solvent.

[0038] Encapsulation of the bioreactor: fix the built-in flat plate 3 on the hollow support 2, put the whole into the reactor shell 1, and add a cover to manufacture the artificial liver bioreactor.

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Abstract

The invention discloses an artificial liver bioreactor comprising an outer shell, a hollow support and built-in hollowed flat plates. The liver cell bioreactor is composed of multiple layers of hollowed flat plates, a circular hole is formed in the middle of each layer of the flat plate and used for allowing the hollowed support to penetrate through and to be fixed, and the flat plates are mutually separated and fixed on the hollow support. The artificial liver bioreactor introduces nanofiber membranes, achieves a three-dimensional latticed structure which has a specific surface area using multiple nanofiber membranes and high porosity and is mutually communicated, can better simulate structural characteristics of natural extracellular matrix than a traditional support materials for technical manufacture, provides good microenvironments for growth of liver cells, is beneficial for cell adhesion and growth, can fix the liver cells, further improves the liver cell density of a biological type artificial liver, and facilitates production of liver cell spheroids. The bioreactor is novel in design, the liver cells are uniformly distributed on the flat plates, and the microenvironments are consistent.

Description

technical field [0001] The invention relates to the technical field of artificial liver support systems, in particular to an artificial liver bioreactor using nanofiber materials as hepatic cell culture. Background technique [0002] Artificial liver is a temporary or partial replacement of liver function by means of extracorporeal mechanical, chemical or biological devices, so as to assist in the treatment of liver insufficiency or related diseases. As an effective auxiliary treatment device for hepatic insufficiency, liver failure or related diseases, artificial liver has great potential in clinical application. [0003] According to the composition and properties of artificial liver, it can be divided into three categories: non-biological artificial liver, biological artificial liver, and hybrid artificial liver. The common methods of non-biological artificial liver treatment are hemodialysis, plasma or whole blood perfusion, blood replacement and filtration, etc., which...

Claims

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Application Information

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IPC IPC(8): A61M1/18A61M1/20
Inventor 冯章启蒋庆
Owner SUN YAT SEN UNIV
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