The present disclosure relates to a liver
support system comprising (a) a single mining column; and (b) a detoxification column comprising a plurality of liver tissues, wherein the liver
support system is configured to allow fluid to flow from the apheresis column to the detoxification column. The detoxification column can provide detoxification,
secretion and regeneration functions. The detoxification may include promotion of severely impaired central
urea cycle as well as CYP function that is crucial for detoxification of exogenous materials,
cellular metabolism, and
homeostasis. The single sampling column can play a role in adsorbing granulocytes from fluid and protecting a plurality of liver tissues from inflammatory injury. The present disclosure relates to methods of using a liver
support system and engineered cells for use in a liver support
system.