Method for detecting developmental toxicity by using caenorhabditis elegans

Abstract

The invention provides a method for detecting developmental toxicity by using caenorhabditis elegans, which belongs to the field of ecological risk evaluation, according to the invention, the method comprises the following steps: using a 24-hole cell culture plate as a main exposure environment, preparing an NGM culture medium with a specific gradient, exposing a test substance to the caenorhabditis elegans eggs on one side of the culture medium, using the sensitivity of caenorhabditis elegans to the external environment change in the development stage from eggs to adults, using escherichia coli food for inducing nematodes to generate movement behaviors, detecting the developmental toxicity according to the characteristics of development and movement, realizing direct observation even without assistance of a microscope, and quickly and qualitatively judging the development toxicity of the tested substance by directly observing the existence and quantity of the food side nematodes withnaked eyes. The time from exposure to index detection is about 24 hours, the index detection time is shortened, naked eye observation and microscopic observation are integrated, and the detection result has high accuracy and reliability.

Description

technical field [0001] The invention relates to the field of ecological risk assessment, in particular to a method for detecting developmental toxicity using Caenorhabditis elegans. Background technique [0002] With the advancement of human science and technology and the continuous improvement of the quality of life, more and more chemicals are used in people's lives, accompanied by a large number of chemicals entering the environment are constantly migrating and transforming. It reflows into water, soil, and the atmosphere, and may gradually accumulate along the food chain, eventually causing health hazards to humans and disturbing the stability of the ecosystem. Therefore, the toxicity detection of chemicals is an important means to assess their health risks and environmental hazards. [0003] Caenorhabditis elegans has many advantages such as precise and short life cycle, small size, easy cultivation, etc. It feeds on bacteria, is non-parasitic, has no harm to humans, a...

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Problems solved by technology

[0004] At present, the toxicity detection of chemicals mostly uses acute lethal toxicity indicators. This method usually adopts the experimental conditions of high concentration and acute exposure, which is inconsistent with the actual situation of low concentration and chronic exposure in the actual environment, and the detection accuracy is relatively low. Therefore, low concentration conditions The environmental significance of toxic effects such as growth and development under the environment is becoming more and more prominent. At the same time, in the traditional method, it needs to be transferred for subsequent index detection. The time spent from the beginning of exposure, transfer operation and subsequent index detection is usually about 36 hours. The developmental toxicity biological detection methods all have shortcomings such as long detection cycle, and it is difficult to promote efficiently

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