Active defense liquid of blaps rynchopetera fairmaire as well as acquisition method and application thereof
A beak-tailed pipa armor and acquisition method technology, applied in the field of insect defense liquid acquisition, can solve the problems of loss, affecting the cytotoxic activity of defense liquid, etc.
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Embodiment 1
[0035] (1) buy the living body of the adult rostral rostral carapace from the market, place it in a plastic basin with smooth edges, lay a layer of dry sand on the bottom, feed chopped vegetables and fruit, the temperature of the raising environment is 20 ℃, and the humidity is 50%. Feed for two days;
[0036](2) Grab the living body of the imago rostral pipa in the step (1) from the back, fix the abdomen and back of the living body of the imago rostral pipa with the thumb and forefinger, contact its tail with the inwall of a 15mm diameter centrifuge test tube, and its tail After being stimulated by the touch of the collection container, the glands at the tail release the defense fluid, which flows down the inner wall of the centrifuge tube by itself;
[0037] (3) When the liquid level of the defense liquid is collected to 1 / 5 of the height of the centrifuge test tube, stand still for 3 minutes, the defense liquid is divided into the upper layer of the defense liquid and the l...
Embodiment 2
[0052] Sample A, sample B and sample C obtained in Example 1 of the present invention were tested for antitumor activity.
[0053] 1. Experimental reagents
[0054] (1) Preparation of thiazolyl blue (MTT) solution
[0055] Accurately weigh 250 mg of thiazole blue powder and mix it with 50 mL of phosphate buffered saline (PBS) to obtain a 5 mg / mL thiazolium blue solution, which should be stored away from light.
[0056] (2) Preparation of triple solution
[0057] Weigh 50g of sodium dodecyl sulfate (SDS) powder, 0.5mL of hydrochloric acid with a mass concentration of 37% and 25mL of isobutanol, dilute with triple distilled water, stir and mix evenly, and transfer the obtained liquid to a capacity of 500mL In the bottle, set the volume to 500mL, mix again, transfer the prepared triple solution to a glass bottle with a blue cap, and store at room temperature.
[0058] (3) Preparation of positive drug solution and test drug solution
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