Active defense liquid of blaps rynchopetera fairmaire as well as acquisition method and application thereof

A beak-tailed pipa armor and acquisition method technology, applied in the field of insect defense liquid acquisition, can solve the problems of loss, affecting the cytotoxic activity of defense liquid, etc.

Active Publication Date: 2018-04-13
DALI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Another method is to use chemical reagents (such as ethanol or methanol) to stimulate the living body of the rostral pipa to make it secrete defense fluid in the chemical reagents, and remove the chemical reagents to obtain defensive secretions; During the process of chemical reagents, some volatile components in the defense solution will be lost, which will affect the cytotoxic activity of the defense solution

Method used

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  • Active defense liquid of blaps rynchopetera fairmaire as well as acquisition method and application thereof
  • Active defense liquid of blaps rynchopetera fairmaire as well as acquisition method and application thereof
  • Active defense liquid of blaps rynchopetera fairmaire as well as acquisition method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] (1) buy the living body of the adult rostral rostral carapace from the market, place it in a plastic basin with smooth edges, lay a layer of dry sand on the bottom, feed chopped vegetables and fruit, the temperature of the raising environment is 20 ℃, and the humidity is 50%. Feed for two days;

[0036](2) Grab the living body of the imago rostral pipa in the step (1) from the back, fix the abdomen and back of the living body of the imago rostral pipa with the thumb and forefinger, contact its tail with the inwall of a 15mm diameter centrifuge test tube, and its tail After being stimulated by the touch of the collection container, the glands at the tail release the defense fluid, which flows down the inner wall of the centrifuge tube by itself;

[0037] (3) When the liquid level of the defense liquid is collected to 1 / 5 of the height of the centrifuge test tube, stand still for 3 minutes, the defense liquid is divided into the upper layer of the defense liquid and the l...

Embodiment 2

[0052] Sample A, sample B and sample C obtained in Example 1 of the present invention were tested for antitumor activity.

[0053] 1. Experimental reagents

[0054] (1) Preparation of thiazolyl blue (MTT) solution

[0055] Accurately weigh 250 mg of thiazole blue powder and mix it with 50 mL of phosphate buffered saline (PBS) to obtain a 5 mg / mL thiazolium blue solution, which should be stored away from light.

[0056] (2) Preparation of triple solution

[0057] Weigh 50g of sodium dodecyl sulfate (SDS) powder, 0.5mL of hydrochloric acid with a mass concentration of 37% and 25mL of isobutanol, dilute with triple distilled water, stir and mix evenly, and transfer the obtained liquid to a capacity of 500mL In the bottle, set the volume to 500mL, mix again, transfer the prepared triple solution to a glass bottle with a blue cap, and store at room temperature.

[0058] (3) Preparation of positive drug solution and test drug solution

[0059] Cisplatin (positive drug) powder, s...

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PUM

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Abstract

The invention provides an acquisition method of active defense liquid of blaps rynchopetera fairmaire. The acquisition method comprises the following steps: firstly providing an adult blaps rynchopetera fairmaire living body; stimulating the adult blaps rynchopetera fairmaire living body to release defense liquid in a physical method; and enabling the obtained defense liquid to stand for 2 to 5 min, wherein the defense liquid is divided into a defense liquid upper layer and a defense liquid lower layer, separating the upper layer and the lower layer, wherein the obtained defense liquid lower layer is the active defense liquid. By adopting the method provided by the invention, the active defense liquid capable of inhibiting the tumor cell proliferation can be successfully obtained; and after the sampling, the blaps rynchopetera fairmaire is still alive and can be raised continuously, and the sampling can be performed again one week later. An experimental result of embodiments shows thatthe active defense liquid can inhibit the tumor cell proliferation, has significant cell toxic activity and can be further developed into an antitumor drug.

Description

technical field [0001] The invention relates to the technical field of insect defense liquid acquisition, in particular to an active defense liquid of the beak-tailed pipa carapace and its acquisition method and application. Background technique [0002] Blaps rynchopetera Fairmaire belongs to Coleoptera (Coleoptera), Tenebrionidae (Tenebrionidae) Blaps. It is an insect medicine with the functions of clearing heat and detoxifying, promoting blood circulation and removing blood stasis, expelling wind and relieving pain, softening and resolving stagnation. Pain, swollen gums, sores, eczema, itchy skin, etc. In Yunnan folk, the beak-tailed pipa armor is often used to treat intractable diseases such as cough, gastritis, boils, and tumors. There are many case reports on the use of stinky shells to cure various cancers in the middle and west of Yunnan Province, and it has been used to treat liver cancer, gastric cancer, rectal cancer, bladder cancer, cervical cancer, breast canc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/64A61P35/00A01K67/033
CPCA01K67/033A61K35/63
Inventor 李玥李贵轲郭娜娜刘熙刘衡马芳芳肖怀祁巧玲李成功
Owner DALI UNIV
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