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nlpik3r1 gene, encoded protein and its application related to the survival, growth and development of brown planthopper

A technology for growth and development of brown planthopper, applied in application, genetic engineering, plant genetic improvement and other directions, can solve the problems of complex genetic diversity of brown planthopper population, lack of ecological control functions, negative impact on farmland ecosystems, etc., and achieve the delay of adult emergence time. , to achieve the effect of pest control and weight loss

Inactive Publication Date: 2017-05-17
CHINA JILIANG UNIV
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  • Description
  • Claims
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Problems solved by technology

The massive abuse of chemical pesticides not only seriously pollutes the environment, but also induces resistance in brown planthoppers (Wang Peng et al., 2013)
Among the pesticides used to control brown planthoppers, the effective pesticides that have played a good role in the past, such as fipronil, imidacloprid, buprofezin, etc., have been eliminated or restricted in use. When carrying out prevention and control, I feel helpless in the choice of pesticides, and chemical control is facing an embarrassing situation that cannot be prevented
The reason is that most of these chemical pesticides aim at large-scale and high-intensity killing of pests. However, due to practical reasons such as the extremely complex genetic diversity of the brown planthopper population, some of them can survive. As a result of high selection pressure, eventually lead to the formation of more adaptive populations
On the other hand, while chemical pesticides kill pests, they will also endanger non-target organisms including natural enemies, which will inevitably have a negative impact on the farmland ecosystem, and the ecological control function has not waited for its due performance

Method used

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  • nlpik3r1 gene, encoded protein and its application related to the survival, growth and development of brown planthopper
  • nlpik3r1 gene, encoded protein and its application related to the survival, growth and development of brown planthopper
  • nlpik3r1 gene, encoded protein and its application related to the survival, growth and development of brown planthopper

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Embodiment 1

[0030] 1 Materials and methods

[0031] 1.1 Nilaparvata lugens for testing

[0032] The source of the tested brown planthoppers is a long-term reared population of insect-sensitive rice variety TN1 in an artificial climate room, with a temperature of 26±2°C, a relative humidity of 80%±5%, and a photoperiod of 16L:8D.

[0033] 1.2 Cloning and verification of the core fragment of the PIK3R1 gene in the brown planthopper

[0034] According to the transcriptome sequence information, the core sequence of the NlPIK3R1 gene of Nilaparvata lugens was obtained and identified by sequence alignment on the NCBI website. The primers NlPIK3R1-F and NlPIK3R1-R (Table 1) were designed using Primer Premier 5.0 software, and the sequences were verified. A 50 μL reaction system was used for PCR amplification, including 25 μL of PCR Mix, 2 μL of 10 μmol / L forward and reverse primers, 2 μL of template, ddH 2 O 19 μL. The PCR reaction program was: 94°C for 4min; 30 cycles of 94°C for 30s, 58°C ...

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Abstract

The invention discloses a brown planthopper survival and growth development-associated NlPIK3R1 gene, and a coded protein and an application thereof. The gene NlPIK3R1 for coding a brown planthopper phosphatidyl inositol 3 kinase p85 alpha subunit comprises a nucleotide sequence as shown in SEQ ID NO:1 and a homologous mutant sequence thereof. The gene has an important function in a survival and growth development process of the brown planthopper, and is restrained in function to cause brown planthopper survival rate drop, slow growth and delayed adult eclosion. The protein coded by the brown planthopper NlPIK3R1 gene comprises an amino acid sequence as shown in SEQ ID NO:2 and a homologous mutant sequence thereof. The gene is restrained in function to cause brown planthopper survival rate drop, slow growth and delayed adult eclosion. RNA interference of the brown planthopper NlPIK3R1 gene is applied to control brown planthopper. RNA interference is successfully performed on the gene, the survival rate of pests is decreased according to the results, the survival and the growth development are obviously restrained, the weight is reduced, and the adult eclosion is delayed.

Description

technical field [0001] The invention relates to a gene related to survival, growth and development of brown planthopper, encoded protein and application thereof. Background technique [0002] Brown planthopper (Nilaparvata lugens ) is a long-distance migratory rice pest, which mainly damages rice plants by sucking phloem sap and spreading rice viruses. In recent years, the brown planthopper has developed into an important pest in the rice region of Asia, with an annual damage area of ​​20 million hectares 2 More than billions of kilograms of paddy output losses are caused (Lin Yongjun et al., 2011). At present, the main emergency control measures against brown planthoppers are spraying chemical pesticides. The extensive abuse of chemical pesticides not only seriously pollutes the environment, but also induces the resistance of brown planthoppers to the long-term application of chemical pesticides (Wang Peng et al., 2013). Among the pesticides used to control brown plant...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/54C12N9/12C12N15/82A01N47/44A01N57/16A01P7/04
Inventor 郝培应俞晓平陆潮峰申屠旭萍马正许益鹏刘光富王正亮
Owner CHINA JILIANG UNIV
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