41results about How to "Susceptible to drug resistance" patented technology

The invention provides a CRISPR-Cas 9 system used for preventing and/or treating hyperlipidemia and other cardiovascular diseases and a preparing method and application thereof. The CRISPR-Cas 9 system comprises an sgRNA sequence of a specific region on a specific targeting human apo CIII gene and a middle carrier comprising the sgRNA sequence, and the specific region on the apo CIII gene is selected from apo CIII gene expressed regions. The invention further provides the application of the CRISPR-Cas 9 system to preparation of drugs for preventing and/or treating hyperlipidemia and other cardiovascular diseases. By means of the prepared sgRNA of the specific targeting human apo CIII gene, the human apo CIII gene can be targeted accurately and knocked out. The preparing method is simple in step, and good in sgRNA targeting, and the knockout efficiency of the CRISPR-Cas 9 system is high.

The invention discloses a medical fertilizer with a function of preventing and treating root-knot nematode and a preparation method as well as application thereof. The medical fertilizer is prepared from the following components in parts by weight: 10 to 75 parts of major element fertilizer, 1 to 10 parts of medium trace element fertilizer, 1 to 10 parts of humic acid-containing raw material, 1 to 10 parts of chitosan oligosaccharide, 0.1 to 10 parts of abamectin, 0.1 to 10 parts of iprodione, 0.1 to 10 parts of fluopyram, 1 to 10 parts of surfactant and the balance of filler, wherein the parts of all the components are 100 parts. The fertilizers, biostimulants such as humid acid and the chitosan oligosaccharide as well as pesticides are compounded, so nutrition can be provided for crops, and root-knot nematode disease can also be effectively prevented and treated; time and labor are saved; the medical fertilizer is a novel environment-friendly product meeting the pesticide and chemical fertilizer zero growth requirement of the Ministry of Agriculture.

The invention relates to novel chloroethyl nitrosourea with anticancer activity and a synthesizing method thereof. The chloroethyl nitrosourea is a compound with a structure of the general formula (I), wherein n is an integer of 2 to 6. The chloroethyl nitrosourea can be used for treating gliomatosis cerebri, colon cancer and leukemia; compared with the existing nitrosourea-type anticancer medicine, the drug resistance is lower, the chemical therapy effect is better, the preparation method is simple in operation, the reaction yield is high, the toxicity is small, the cost is low, the method is suitable for the industrialized production, the development prospect is wide, and the application value is good.

The invention provides an ecological chicken raising method for resisting diseases and promoting growth and relates to the technical field of breeding. The chicken raising method comprises the following steps of raising place selection, feeding and management, disease prevention and control and henhouse management. The chickens raised by adopting the ecological chicken raising method is high in survival rate and strong in disease-resistant ability, obtained chicken has firm muscles, fine and tender meat, a delicious taste, few subcutaneous fat, delicious, sweet and fragrant taste and rich nutrients, and the ecological chicken raising method is simple and convenient to operate, low in cost, easy to implement and suitable for various scales of farms.

The invention discloses a compound sulphamethoxazole injection and a preparation method thereof. The injection is prepared from 5-20 % of the main drug sulphamethoxazole, 2-10 % of flunixin meglumine, 1-4 % of trimethoprim, 0.2-0.4 % of an accessory anti-oxidant, 40-70 % of an organic solvent, 0.5-2 % of an adjusting reagent and the balance being water for injection. The injection prepared by the invention can provide effective treatment for secondary infections and mixed infections of streptococcus diseases, toxoplasmosis, eperythrozoonosis, E. coli diseases, asthma and other diseases, is obvious in long-term effects, with a drug effect being enhanced by several times to dozens of times, small in application amount, few in drug using times, little in irritation and fast in performance of drug effects, saves production cost, increases added value of products, and reduces toxic and side effects due to drug mismatch during treatment.

The invention provides a pharmaceutical composition used for comprehensive prevention of primary tumor metastasis after exairesis. The pharmaceutical composition is composed of five kinds of drugs, which are capable of increasing body internal tumor immune functions, inhibiting marrow internal circulation tumor cell activity, inhibiting adhesion and implantation of circulating tumor cells in blood onto tunica elastica interna, preventing aggregation of circulating tumor cells in blood vessels, and strengthening tissue matrix structures respectively. Each of the five drug is capable of controlling a different point related to tumor metastasis. Optimized combination of the five drugs is capable of preventing tumor cells from metastasis after exairesis effectively and safely.

The invention discloses a bivalent freeze-dried egg yolk antibody for infectious feline rhinitis-conjunctivitis and feline panleukopenia and a preparation method and an application thereof, and belongs to the technical field of veterinary biopharmaceuticals. The bivalent freeze-dried egg yolk antibody for the infectious feline rhinitis-conjunctivitis and the feline panleukopenia can be directly prepared by bivalent vaccine immunized laying hens without hurting animals, and is simple in process method and low in cost; and the obtained bivalent antibody is high in titer, good in stability, quick to take effect, free of producing drug resistance, capable of being used for rapid treatment or emergency prevention of the infectious feline rhinitis-conjunctivitis and the feline panleukopenia and high in protection rate. Compared with vaccines and antibiotics, the invention has wider and more practical application prospects.

The invention provides a method and process for preparing tung oil pesticide. The method comprises the following steps: refining tung oil with alkali, mixing with an emulsifier and uniformly mixing with other auxiliaries. The tung oil has effects of killing pests and inhibiting bacteria. The tung oil pesticide is simple in preparation, has a good pest killing effect and wide market application prospect, has the characteristics of fast action and long action, and can be used for reducing the usage of chemical pesticide and preventing pests from generating drug resistance. The tung oil pesticide does not have pollution in production and processing processes, has stable product quality, wide insecticidal spectrum, strong activity and convenience in use, and can be used for preventing and controlling beauveria bassiana, argyroneta aquatica, cabbage caterpillar, helicoverpa armigera, aphid, green leafhopper, cockroaches, acarid and the like.

The invention relates to a compound sulfamethoxypyridazine injection and a preparation method thereof. The synergistic sulfamethoxypyridazine injection has enduring drug effect and obvious curative effect. The injection comprises the following components in parts by weight: 10-20 parts of sulfamethoxypyridazine, 2-4 parts of indometacin, 2-4 parts of trimethoprim, 5-10 parts of polyethyleneglycol 8000, 30-40 parts of propanediol, 0.1-0.2 part of antioxidant, 30-55 parts of water for injection, and sufficient amount of pH regulator for regulating the pH value of the system to 9.5-10.5. The preparation method comprises the following steps: adding the antioxidant into the water for injection to prepare a solution A, adding the polyethyleneglycol 8000, sulfamethoxypyridazine, indometacin and trimethoprim into the propanediol, and completely dissolving by stirring to prepare a solution B; and merging, filtering, packaging and sterilizing to obtain the finished product. The injection provided by the invention is a new drug preparation with triple effects, and has the advantages of long drug effect, low consumption, low application frequency, small irritation, quick drug effect taking and wide curative range.

The invention discloses a nauclea offcinalis extract, which is prepared by the following steps: grinding the nauclea offcinalis into coarse powder, feeding into an extraction kettle, adopting supercritical carbon dioxide to extract, and collecting extracts to obtain a supercritical extract; carrying out water extraction on the supercritical dregs, and condensing to obtain a water extract; combining and uniformly mixing the water extract and the supercritical extract to obtain the nauclea offcinalis extract; drying the nauclea offcinalis extract to obtain dried plaster powder, and preparing the dried plaster powder to the necessary dosage form. Additionally, the invention discloses an application of the nauclea offcinalis extract in treatment of pelvic inflammation. The invention has the following advantages: the prescription provided by the invention is more significant in treatment effect, the improved situation of the pelvic inflammation is better than a control group, thereby showing that the treatment effect of the prescription for the pelvic inflammation is better than that of the Western medicine metronidazole. Due to the fact that the pelvic inflammation is easy to relapse, so that the long-term medication is needed, but most Western medicines are based on antibiotic treatment, the side-effect is high and the drug resistance is easy to produce; however, the Chinese patent drug is low in the side-effect and suitable for the long-term medication, thereby being fitting for the characteristic that the pelvic inflammation is easy to relapse; and the prescription provided by the invention is appropriate for the long-term treatment of the pelvic inflammation.

The invention discloses a nerve-calming moisturizing mask and a preparation method thereof. The nerve-calming moisturizing mask is prepared from the following raw materials in parts by weight: 3 to 6 parts of hexadecanol, 2 to 4 parts of biological refined glycerinum, 2 to 4 parts of sulfonated castor oil, 2 to 5 parts of fatty alcohol-polyoxyethylene ether, 0.05 to 0.3 part of hyaluronic acid, 0.2 part of paraben, 30 to 40 parts of a sedative and 40 to 60 parts of pure water, wherein the sedative consists of a traditional Chinese medicine extract and lavender essential oil according to the volume ratio of (80-200):1; d the traditional Chinese medicine extract is prepared from the following raw materials in parts by weight: 10 to 20 parts of aizoon stonecrop herb, 10 to 20 parts of sunflower seeds, 20 to 30 parts of peanut leaves and 20 to 30 parts of spina date seeds. The nerve-calming moisturizing mask provided by the invention contains natural traditional Chinese herbal medicine essence, hyaluronic acid and biological refined glycerinum which are permeated into skin quickly, can improve sleep, moisturize and tender skin, have low irritability on skin and avoid allergy.

Compounds useful in the treatment of metastatic melanoma and other cancers containing a triazine ring scaffold are described. These compounds may be classified into two groups: (1) two disubstituted triazine rings are covalently linked by an organic linker to each other and (2) one trisubstituted triazine ring.

The invention discloses an application technology of itaconic acid derivatives in herbicide emulsifiable concentrate. The herbicide emulsifiable concentrate comprises itaconic acid derivatives, herbicide effective ingredients, an emulsifier, a stabilizer, a pH regulator and a solvent. The itaconic acid derivatives are prepared by the reaction of itaconic acid with triethylamine, and the molar ratio of the itaconic acid to the triethylamine is 1:1. A herbicide emulsifiable concentrate system formulated with the itaconic acid derivatives replaces non-environmentally friendly solvents, consumption of high-toxicity organic solvents such as benzene is reduced, environmental pollution is alleviated, and herbicidal effect is improved.

The invention relates to a compound sulfamethoxypyridazine injection and a preparation method thereof. The synergistic sulfamethoxypyridazine injection has enduring drug effect and obvious curative effect. The injection comprises the following components in parts by weight: 10-20 parts of sulfamethoxypyridazine, 2-4 parts of indometacin, 2-4 parts of trimethoprim, 5-10 parts of polyethyleneglycol 8000, 30-40 parts of propanediol, 0.1-0.2 part of antioxidant, 30-55 parts of water for injection, and sufficient amount of pH regulator for regulating the pH value of the system to 9.5-10.5. The preparation method comprises the following steps: adding the antioxidant into the water for injection to prepare a solution A, adding the polyethyleneglycol 8000, sulfamethoxypyridazine, indometacin and trimethoprim into the propanediol, and completely dissolving by stirring to prepare a solution B; and merging, filtering, packaging and sterilizing to obtain the finished product. The injection provided by the invention is a new drug preparation with triple effects, and has the advantages of long drug effect, low consumption, low application frequency, small irritation, quick drug effect taking and wide curative range.

A natural sterilization insecticide utilizes sumac leaves or extracts thereof as a sterilization insecticide; the sterilization insecticide includes the sumac leaves or the extracts thereof. The leaves of sumac trees or the extracts thereof have the efficacies of inhibiting microbial breeding, and effectively killing pathogenic bacteria and injurious insects. The natural sterilization insecticide has the advantages of simple preparation, good effect of killing the pathogenic bacteria and injurious insects, and broad market application prospect. The invention relates to the sumac leaves or the extracts thereof for killing the pathogenic bacteria and injurious insects and preferably for plant pesticides and fungicides.

The invention discloses traditional Chinese medicine tooth powder. The traditional Chinese medicine tooth powder comprises 9-17 parts of traditional Chinese medicine composition, 1.5-2 parts of surfactant, 0.02-0.06 part of sweetening agent, 18-30 parts of abrasive and 0.02-0.08 part of perfume, wherein the traditional Chinese medicine composition comprises 1.8-2.5 parts of rhizoma belamcandae, 2-3 parts of hedyotis diffusa, 1-2 parts of semen juglandis, 0.2-0.5 part of piper cubeba, 0.2-0.3 part of radix scutellariae, 0.1-0.2 part of radix polygonati officinalis, 0.5-1.5 parts of lotus root,0.5-1 part of semen sterculiae lychnophorae, 1-2 parts of pericarpium citri reticulatae, 1-2 parts of Eucommia ulmoides, 0.1-0.2 part of tabasheer, 0.1-0.3 part of fructus caryophylli and 0.5-1.5 parts of red yeast rice. The traditional Chinese medicine tooth powder prepared by mixing various Chinese herbal medicine materials is capable of cleaning the teeth, preventing tooth decay, clearing awayheat and toxic materials, resisting bacteria, diminishing inflammation and relieving pain, preventing dental ulcer and gum bleeding, protecting the throat and relieving sore-throat, and the like.

The invention discloses an insect pest control method for a medicinal plant cultivation base. The method comprises following steps: (1) performing high-temperature steam sterilization for crushed and mixed soil matrixes so as to reduce eggs in the soil; (2) for platform beds and ground beds of greenhouses, employing lack light lamps to trap and kill insect pests; (3) for field ridges and seedbeds attacked by the insect pests, mixing a chlorpyrifos solution which is diluted by 2000 times with cabbage leaves, and placing the mixture near the field ridges and seedbeds; and (4) for field ridges and seedbeds attacked by fungus gnats, employing yellow boards and blue boards to trap and kill fungus gnat adults. Through the combination of physical control and chemical control, insect pests, such as cutworms, scarabs, sciarid flies and soil root-knot nematodes, which severely do harm to seeds, seedlings and adults of pairs polyphylla and panax japonicas majoris can be targetly and comprehensively controlled, the method is low is cost and is easy to perform, and the method can be used for bases and farmer households on a large scale.

The invention discloses a traditional Chinese medicine composition for treating type II diabetes. The traditional Chinese medicine composition is prepared from, by weight, 13-17 parts of Radix Rehmanniae Preparata, 8-12 parts of Herba Dendrobii, 4-8 parts of Rhizoma Coptidis, 1-5 parts of Rhizoma Zingiberis, 8-12 parts of Fructus Mume, 13-17 parts of Radix Ginseng, 8-12 parts of Herba Gynostemmatis Pentaphylli, 4-8 parts of Pheretima, 4-8 parts of Bombyx Batryticatus and 4-8 parts of Radix Glycyrrhizae. The traditional Chinese medicine composition has efficacies of internal organ nourishing, Qi movement regulating, blood glucose reducing, stasis removing and the like and is capable of making the five internal organs tough and coordinated through Qi tonifying and harmonizing of the five internal organs; by combination of syndrome differentiation and five-element five-flavor theories for treating diabetes, main clinical symptoms of patients suffering from type II diabetes with deficiencyof both Qi and Yin can be effectively improved; in addition, effectiveness in blood sugar reduction and glucostasis control can be achieved, and safety in use, freeness of toxic and side effects andavailability for long-term utilization are realized.

The invention discloses special preparation of cnidium lactone for improving sarcopenia and muscular atrophy after fracture, belongs to the technical field of application of traditional Chinese medicines, and aims to improve sarcopenia and muscular atrophy after fracture after special preparation of fructus cnidii. A special preparation method of the fructus cnidii comprises the following steps: dissolving cnidium lactone in DMSO (dimethylsulfoxide), blowing, beating and uniformly mixing, and after the cnidium lactone is dissolved, adding the cnidium lactone into corn oil to prepare an injection; and putting into an oscillator, and setting the temperature to be 37.0 DEG C and the frequency to be 230r/min for 8 hours per night. The special preparation of the cnidium lactone provided by the invention has a biological effect of improving muscle, so that sarcopenia and muscle atrophy after fracture are treated.

The invention discloses a herbicide containing quinclorac, pyrazosulfuron-ethyl and fenoxaprop-p-ethyl. The herbicide is prepared from the following components in percentage by mass: 30 to 60 percentof quinclorac, 5 to 20 percent of pyrazosulfuron-ethyl, 5 to 15 percent of fenoxaprop-p-ethyl, and agriculturally acceptable auxiliaries including calcium lignosulphonate, carrier kaolin and white carbon black. The preparation method comprises the following steps: weighing quinclorac, pyrazosulfuron-ethyl, fenoxaprop-p-ethyl, calcium lignosulphonate, white carbon black and kaolin according to a given amount, adding into a high-speed stirrer, uniformly mixing and stirring, and crushing by adopting a jet mill to obtain a wettable powder product. The use method comprises the following steps: in the 7-8-leaf stage of weeds after winter, weighing the herbicide according to the ratio of 40-70g/mu, and adding water for use. The herbicide is high in safety, low in environmental pollution, wide inweeding spectrum and lasting in pesticide effect, and can be applied to lawn weeding; the weeding composition can also be used for annual weeds in direct-seeding rice fields or transplanting fields.