32 results about "Autotransfusion" patented technology

The invention relates to the biological cell technology field, particularly a method for separating and culturing miniature swine bone marrow-derived endothelial progenitor cells. The conventional EPCs culture method has the disadvantages of complexity and greatly reduced cell harvest rate by using immunomagnetic bead separation. The method in the invention comprises the steps of directly using myelomonocyte to conduct culture in vitro to obtain EPCs, adding growth factors in culture solution, planting in a definite density in a culture flask/ vessel or a glass sheet enveloped by fibronectin, and conducing appropriate culture to obtain EPC. The method with high repeatability simplifies the steps of the conventional culture method, effectively avoids unnecessary cell loss caused by the conventional separation and culture method, increases the pick-up rate of EPCs, and saves a large amount of funds. The method of the invention also prepares for clinical application in future, and can reduce the usage of bone marrow and provide a technology platform for curing traumatic or ischemic diseases by autotransfusion.

A blood-collection system configured to permit selection of either collection and disposal of shed blood from a patient or collection and processing of shed blood for autologous transfusion back to the patient includes an automated blood-processing machine. The system may also include a blood-collection reservoir, a separation chamber, and a fluid conduit. The blood-collection reservoir may be engageable with the blood-processing machine so that the machine can identify and report the presence of blood in the reservoir. The separation chamber may also be engageable with the blood processing machine. The fluid conduit may be configured to selectively join the reservoir to the separation chamber by a quick-connect coupling.

The venous reservoir is omitted from an extracorporeal blood circuit system by substituting a blood reservoir in parallel with the arterial pump. The reservoir has an inlet connected to the outlet of the pump and an outlet connected to the inlet of the pump. At least three blood flow paths can be configured. A main path has the inlet and outlet of the reservoir blocked while pumped blood flows through the venous cannula, the oxygenator, and the arterial cannula. An accumulator path has the inlet to the oxygenator and the outlet of the reservoir blocked while blood flows through the venous cannula and the inlet to the reservoir. A discharge path has the inlet of the reservoir blocked while the outlet is open whereby blood from the reservoir flows into the oxygenator. In a fourth configuration, the ECC fluid can be recovered in the reservoir and hemoconcentrated for autologous transfusion.

The invention provides autologous stem cells for treating renal insufficiency and a preparation method thereof. In the preparation method, autologous progenitor cells for treating renal insufficiency are generated by induced differentiation of adipose-derived stem cells which are obtained by separation from the adipose tissue of a patient, purification and amplification culture. The resource of the autologous progenitor cells is rich, the autologous progenitor cells can be obtained conveniently, and the preparation method is simple, convenient, easy, economic and practical. The prepared stem cells are subjected to autotransfusion to treat renal insufficiency with obvious treatment effect, high safety, no immunological rejection, no obvious toxic or side effects, no adverse reaction and less pain in patients. The invention provides a brand-new treatment means for patients with renal insufficiency and has a profound active significance.

An apparatus for collecting and salvaging blood is disclosed, comprising: a first chamber for collecting blood through an inlet port; a second chamber for receiving blood from the first chamber through a connection channel and a channel valve manually controllable to close the connection channel. Further, the apparatus comprises a control means for simultaneously controlling the channel valve and the internal pressure of the second chamber. Still further, a lipid separator is arranged in the second chamber for separating the blood from lipids before the release through the release port. By the arrangement of the lipid separator in the second chamber, it is possible to provide a very efficient separation and cleaning of the blood, and to allow release of the separated lipids and other buoyant substances from the chamber when the concentration is too high. Consequently, the apparatus could be used during an extended period of time, and could also be used for repeated re-use. The manually controllable channel valve could be made very reliable and efficient. At the same time the control means for simultaneously controlling the channel valve and the internal pressure of the second chamber provides means for very efficient operation of the apparatus. With one single operation, and by use of only one hand, the operator could switch between two operation modes: a first operation mode in which the channel valve is open and the second chamber is subjected to low pressure, preferably vacuum, whereby blood is conveyed from the first chamber to the second chamber through the channel valve; and a second operation mode in which the channel valve is closed and the second chamber is subjected to ambient air pressure, whereby blood is drained from the second chamber while continuing to collect blood in the first chamber.

The invention discloses a suspended retransfusion set, and belongs to the technical field of medical equipment and supplies. A clinical washing type autotransfusion technology has allogeneic transfusion shortcomings and plasma protein and coagulation factor loss; a simple method of manual filtering operation by multiple operators is frequently adopted for the conventional filtering type autotransfusion technology, and is exposed and high in pollution rate. The suspended retransfusion set consists of a negative-pressure suction pipeline, a blood recovery pipeline, a retransfusion pipeline and an automatic anticoagulant transfusion device, wherein the negative-pressure suction pipeline consists of a negative-pressure pipe, a negative-pressure conversion bottle, a negative-pressure indicator and a regulator, and is communicated with an upper filter screen gap through an internal opening of the negative-pressure pipe; the blood recovery pipeline comprises a suction head, a blood inlet pipe, a coarse filter screen, a fine filter screen, the upper filter screen gap, a blood storage tank, a blood storage tank air inlet pipe, a blood transfusion three-way valve and the like; the automatic anticoagulant transfusion device comprises a medicine storage tank, a medicine transfusion pipe, a medicine transfusion pipe grading valve, a blood inlet pipe-medicine transfusion pipe synchronization valve and the like. The suspended retransfusion set has the advantages of integrated design, automatic anticoagulant transfusion function, high recovered blood quality, high operability, high emergent treatment performance and the like.

The invention relates to an autotransfusion machine. The autotransfusion machine consists of three parts of a machine body, casters and a support. The support is fixedly installed on the rear side end of the upper surface of the machine body; a display screen is positioned in a middle position on the upper surface of the machine body, an emergency switch and a power switch are fixed on the two sides of the display screen on the upper part of the machine body, and a peristaltic pump and a centrifuge cup are installed on the machine body in the front of the display screen; a material storage box is arranged on the lower part of the machine body, a lateral sliding rail is positioned above the material storage box on the front part of the machine body, and a suspension head is fixedly connected onto the lateral sliding rail and slides along the sliding rail left and right; and a safety power socket and a handle are arranged on the rear part of the machine body, and the casters are fixedly installed on the four corners of the bottom of the machine body and respectively provided with a self-lock device, so that the autotransfusion machine can be conveniently moved towards all directions in a use process. The autotransfusion machine is simple in structure and has multiple use functions; the angle of the display screen can be adjusted, so that the autotransfusion machine is convenient to operate and can quickly read data; a host automatically stores all the data so as to facilitate the checking in the operation or after operation; and the autotransfusion machine conveniently and quickly provides a great quantity of return blood for patients.

The invention relates to an establishing method of a pregnancy late trimester storing type autologous component blood transfusion system. A 300 ml sealing three-connecting-bag system is used. The anticoagulant capacity in a sealing three-connecting bag is normal capacity, 300 ml autologous blood is collected, the autologous blood is made into blood components in six hours, and the fact that a whole process is kept in a cold chain state is guaranteed. The blood components are suspension erythrocytes and frozen fresh plasma. A scientific basis is provided for establishing of the pregnancy late trimester storing type autologous blood transfusion standard, accordingly, storing type autologous blood transfusion can be well used in obstetrics patients, safety, effectiveness and economy are achieved, and therefore promotion and application of storing type autologous blood transfusion in obstetrics are well achieved.

The invention provides a hand-pushed autologous transfusion blood collecting trolley, and belongs to the field of medical apparatus and instruments. The hand-pushed autologous transfusion blood collecting trolley is a medical blood collecting auxiliary device, is used for blood collection of hospital autologous transfusion, is mainly used for solving a problem that no special shaking device is provided in autologous transfusion blood collection in the prior art, is obtained via reasonable combination of a medical trolley and a shaking device, is provided with an appropriate power supply, is suitable to be applied in blood collection of hospital autologous transfusion, is convenient and reliable, and is capable of saving manpower and time, and reducing labour intensity.

The invention discloses a method for clinically improving breast cancer through TIL. The method comprises the steps that firstly, excising a tumor from the body of a patient; then transporting the tumor tissue to a laboratory under sterile and cold-chain conditions, locally separating tumor infiltrating lymphocytes from the tumor by using a mechanical treatment and enzyme digestion method, and separating to obtain initial tumor infiltrating lymphocytes; adding high-dose IL-2 for in-vitro culture and transformation, amplifying the initial tumor infiltration lymphocyte by ten thousand times, further purifying, and performing cryopreservation; finally, carrying out autotransfusion of the tumor infiltrating lymphocytes into the body of the biliary duct cancer patient, wherein the number of injected into the tumor infiltrating lymphocytes each time is 5 * 10 < 9 > to 5 * 10 < 10 >. According to the invention, lymphocytes in breast tumor tissues are separated and amplified, and then are fedback to the patient, so that the purpose of improving the living quality of the breast cancer patient can be achieved. The TIL tumor infiltration lymphocyte can recognize T cells of tumor cell neoantigens and has the capacity of efficiently killing tumor cells.