32 results about "Autotransfusion" patented technology

An apparatus and methods for enhanced plasmapheresis comprising a filter membrane under an orbital motion or movement that has optimal local shear forces and maximum plasma flow output. The methods for biological separation and therapies comprise platelet collection, viral particle removal, cell washing and processing for stem cell selection, bone marrow purging, red blood cell collection, auto-transfusion, auto-immune disease treatment, selective macro-molecule removal, toxin removal, LDL removal, extracorporeal plasma delipidation, and the like.

A device for autologous blood transfusion includes a centrifuge unit with an autotransfusion set rotatably mounted thereto. The autotransfusion set includes a separation unit for concentrating a cell fraction by centrifugation and a tubing system for providing a connection to a blood supply. The tubing system includes a blood supply line leading to the separation unit for supplying blood to be processed and a return line leading away from the separation unit. A filter is integrated into the autotransfusion set in order to eliminate leukocytes and / or tumor cells, thereby yielding greater safety in autotransfusion.

The invention relates to the biological cell technology field, particularly a method for separating and culturing miniature swine bone marrow-derived endothelial progenitor cells. The conventional EPCs culture method has the disadvantages of complexity and greatly reduced cell harvest rate by using immunomagnetic bead separation. The method in the invention comprises the steps of directly using myelomonocyte to conduct culture in vitro to obtain EPCs, adding growth factors in culture solution, planting in a definite density in a culture flask / vessel or a glass sheet enveloped by fibronectin, and conducing appropriate culture to obtain EPC. The method with high repeatability simplifies the steps of the conventional culture method, effectively avoids unnecessary cell loss caused by the conventional separation and culture method, increases the pick-up rate of EPCs, and saves a large amount of funds. The method of the invention also prepares for clinical application in future, and can reduce the usage of bone marrow and provide a technology platform for curing traumatic or ischemic diseases by autotransfusion.

A blood-collection system configured to permit selection of either collection and disposal of shed blood from a patient or collection and processing of shed blood for autologous transfusion back to the patient includes an automated blood-processing machine. The system may also include a blood-collection reservoir, a separation chamber, and a fluid conduit. The blood-collection reservoir may be engageable with the blood-processing machine so that the machine can identify and report the presence of blood in the reservoir. The separation chamber may also be engageable with the blood processing machine. The fluid conduit may be configured to selectively join the reservoir to the separation chamber by a quick-connect coupling.

The venous reservoir is omitted from an extracorporeal blood circuit system by substituting a blood reservoir in parallel with the arterial pump. The reservoir has an inlet connected to the outlet of the pump and an outlet connected to the inlet of the pump. At least three blood flow paths can be configured. A main path has the inlet and outlet of the reservoir blocked while pumped blood flows through the venous cannula, the oxygenator, and the arterial cannula. An accumulator path has the inlet to the oxygenator and the outlet of the reservoir blocked while blood flows through the venous cannula and the inlet to the reservoir. A discharge path has the inlet of the reservoir blocked while the outlet is open whereby blood from the reservoir flows into the oxygenator. In a fourth configuration, the ECC fluid can be recovered in the reservoir and hemoconcentrated for autologous transfusion.

An autotransfusion device having: a first line and a first pump, the first line being connected to the inlet of a blood centrifugation cell, and branches of the first line being (i) connected to a red blood cell container, (ii) provided with a coupling, and (iii) being suitable to be connected to the patient; a second line connected to the outlet of a blood centrifugation cell and two branches of the second line being provided with two couplings; and a third line and a second pump, the third line being connected to the first line in an intermediate position between the first pump and the blood centrifugation cell, and branches of the third line (i) being connected to a physiological solution container and (ii) being provided with a coupling.

The invention provides autologous stem cells for treating renal insufficiency and a preparation method thereof. In the preparation method, autologous progenitor cells for treating renal insufficiency are generated by induced differentiation of adipose-derived stem cells which are obtained by separation from the adipose tissue of a patient, purification and amplification culture. The resource of the autologous progenitor cells is rich, the autologous progenitor cells can be obtained conveniently, and the preparation method is simple, convenient, easy, economic and practical. The prepared stem cells are subjected to autotransfusion to treat renal insufficiency with obvious treatment effect, high safety, no immunological rejection, no obvious toxic or side effects, no adverse reaction and less pain in patients. The invention provides a brand-new treatment means for patients with renal insufficiency and has a profound active significance.

An apparatus for collecting and salvaging blood is disclosed, comprising: a first chamber for collecting blood through an inlet port; a second chamber for receiving blood from the first chamber through a connection channel and a channel valve manually controllable to close the connection channel. Further, the apparatus comprises a control means for simultaneously controlling the channel valve and the internal pressure of the second chamber. Still further, a lipid separator is arranged in the second chamber for separating the blood from lipids before the release through the release port. By the arrangement of the lipid separator in the second chamber, it is possible to provide a very efficient separation and cleaning of the blood, and to allow release of the separated lipids and other buoyant substances from the chamber when the concentration is too high. Consequently, the apparatus could be used during an extended period of time, and could also be used for repeated re-use. The manually controllable channel valve could be made very reliable and efficient. At the same time the control means for simultaneously controlling the channel valve and the internal pressure of the second chamber provides means for very efficient operation of the apparatus. With one single operation, and by use of only one hand, the operator could switch between two operation modes: a first operation mode in which the channel valve is open and the second chamber is subjected to low pressure, preferably vacuum, whereby blood is conveyed from the first chamber to the second chamber through the channel valve; and a second operation mode in which the channel valve is closed and the second chamber is subjected to ambient air pressure, whereby blood is drained from the second chamber while continuing to collect blood in the first chamber.

The present invention provides a method for increasing double-yolk egg laying rate of Gaoyou duck by means of self-body blood transfusion. Said method includes the following steps: in the laying season of Gaoyou duck drawing 5-10 ml of blood from wing root vein of every Gaoyou duck, marks its, placing the blood into a sterile plugged plastic test tube in which the anticoagulant preservative fluid is contained, storing them below 4 plus or minus 1 deg.C, after 10 days, injecting said blood into correspondent duck body interior.

The invention relates to a building method of a preoperative autologus blood donation system for late trimester of pregnancy. A 300ml sealing triple bag system is adopted, the content of anticoagulants in a blood bag is increased, autologus blood of 200 ml is collected, and the anticoagulants and the blood are mixed evenly after the blood is collected and then are stored in a blood storage refrigerator of 4+ / -2.0 DEG C; the blood storage time is 15-35 days before the surgery is performed; objects with autologus blood collected are puerperae with the autologus blood donation indications, and the autologus blood donation indications are puerperae who do not have presentations of parturition signs, vaginal bleeding, growth restriction of fetuses or intrauterine hypoxia and are stable in state of illness after diseases of a cardiovascular system, diabetes, hypertensive disorder complicating pregnancy, infectious diseases and medical complications are excluded. The scientific evidence is provided for standard building of a preoperative autologus blood donation technology for late trimester of pregnancy, and therefore preoperative autologus blood donation is better applied to patients of obstetrics, the system is safer, more effective and more economical, and application and popularization of the preoperative autologus blood donation in the obstetrics are better promoted.

Systems and methods are described for salvaging red blood cells from patients during a surgical procedure. In one general aspect, a system is described for extracting blood from blood-soaked absorbent surgical materials such as surgical sponges, gauze, tape, and the like. The collected blood and fluids from these materials can be transferred to a cell salvage machine for harvesting viable red blood cells for autotransfusion.

An autotransfusion device having: a first line and a first pump, the first line being connected to the inlet of a blood centrifugation cell, and branches of the first line being (i) connected to a red blood cell container, (ii) provided with a coupling, and (iii) being suitable to be connected to the patient; a second line connected to the outlet of a blood centrifugation cell and two branches of the second line being provided with two couplings; and a third line and a second pump, the third line being connected to the first line in an intermediate position between the first pump and the blood centrifugation cell, and branches of the third line (i) being connected to a physiological solution container and (ii) being provided with a coupling.

Apparatus for blood processing comprising a blood collection reservoir (30), a blood collection conduit, a blood transfer conduit, and a manually operable pump unit (10) comprising first and second peristaltic pumps (10A, 10B). The first peristaltic pump is mounted about and acts upon the blood collection conduit. The second peristaltic pump is mounted about and acts upon the blood transfer conduit. The pump unit is provided with an actuator (112) adapted to switch the second peristaltic pump between an operative condition and an inoperative condition: in the operative condition the second peristaltic pump is engaged with the blood transfer conduit to convey blood from the blood collection reservoir while the first peristaltic pump is engaged with the blood collection conduit to convey blood from a wound site to the blood collection reservoir, and in the inoperative condition the second peristaltic pump is disengaged from the blood transfer conduit.

The present invention discloses an application of a B cell vaccine in preparation of drugs for treating rheumatoid arthritis and the B cell vaccine having the application. Compared with drug treatment, the B cell vaccine has the following advantages of being smaller in dosage, lower in administration frequency, longer in maintenance period, and simple and convenient in subcutaneous injection, cantreat diseases through autoimmune regulation functions, can effectively control the diseases, reduces bone erosion and joint destruction, and is free of immune tolerance problems in autotransfusion and small in side effects.

An apparatus for collecting and salvaging blood includes: a first chamber with a below atmosphere internal pressure for continuous collection of blood through an inlet port; a second chamber for receiving blood from the first chamber through a connection channel; a channel valve manually controllable to close the connection channel; and a control for simultaneously controlling the channel valve and the internal pressure of the second chamber between an internal pressure corresponding to the internal pressure of the first chamber and ambient air pressure, wherein the control is arranged to provide an internal pressure corresponding to the internal pressure of the first chamber to the second chamber through a passageway separated from the connection channel for providing blood from the first chamber to the second chamber.

The invention discloses autologous blood transfusion equipment. The autologous blood transfusion equipment comprises a collecting groove, an anticoagulant bag, a blood recovery machine, a weight measuring instrument, a temperature detector, a sterilizer, a recording printer controller, a controller, a displayer, an alarm device and an operating bed. A sieving-leaking groove is formed in one end ofthe surface of the operating bed. Through the collecting groove, blood of a patient is collected and subjected to recovery treatment through the blood recovery machine, and in order to prevent bloodclotting during collection, an anticoagulant is added into the collecting groove. The collected blood is subjected to weight measuring through the weight measuring instrument, and if the weight of theblood meets the demand for recovery, the controller will not send signals to the alarm device, and the alarm device will not give alarms; otherwise, alarm reminding is performed to tell that the collected blood is insufficient and can not meet the demands of the patient. The temperature inside the blood recovery machine and the liquid level of the collected blood can be displayed on the displayerafter being processed by the controlled, medical workers can learn about the conditions of blood recovery through the displayer, and the problem that blood sources are insufficient is solved for thepatient.

The invention discloses a method for clinically improving gastric cancer by TIL. The method comprises the following steps: separating lymphocytes from tumor tissues, amplifying the lymphocytes, culturing mature TIL cells, and injecting the TIL cells back to a gastric cancer patient for improving the living quality of the gastric cancer patient. The tumor infiltration lymphocyte disclosed by the invention has the advantages that tumor infiltrating lymphocytes are separated from a local part of a tumor by using a mechanical treatment and enzyme digestion method, high-dose IL-2 is added for in-vitro culture and transformation, and then the tumor infiltrating lymphocytes are subjected to autotransfusion into the body of the patient, so that compared with other immunotherapy, the tumor infiltrating lymphocytes have the advantages of multiple tumor antigen recognition targets, few side effects, multiple treatable solid tumors, stronger curative effects and the like. The TIL immunotherapy provided by the invention has efficient killing ability, can identify a plurality of antigen targets, uses autoimmune cells, has no side effect, and can be used for patients with recurrent or metastaticadvanced gastric cancer.

The invention discloses a suspended retransfusion set, and belongs to the technical field of medical equipment and supplies. A clinical washing type autotransfusion technology has allogeneic transfusion shortcomings and plasma protein and coagulation factor loss; a simple method of manual filtering operation by multiple operators is frequently adopted for the conventional filtering type autotransfusion technology, and is exposed and high in pollution rate. The suspended retransfusion set consists of a negative-pressure suction pipeline, a blood recovery pipeline, a retransfusion pipeline and an automatic anticoagulant transfusion device, wherein the negative-pressure suction pipeline consists of a negative-pressure pipe, a negative-pressure conversion bottle, a negative-pressure indicator and a regulator, and is communicated with an upper filter screen gap through an internal opening of the negative-pressure pipe; the blood recovery pipeline comprises a suction head, a blood inlet pipe, a coarse filter screen, a fine filter screen, the upper filter screen gap, a blood storage tank, a blood storage tank air inlet pipe, a blood transfusion three-way valve and the like; the automatic anticoagulant transfusion device comprises a medicine storage tank, a medicine transfusion pipe, a medicine transfusion pipe grading valve, a blood inlet pipe-medicine transfusion pipe synchronization valve and the like. The suspended retransfusion set has the advantages of integrated design, automatic anticoagulant transfusion function, high recovered blood quality, high operability, high emergent treatment performance and the like.

Apparatus for blood processing comprising a blood collection reservoir (30), a blood collection conduit, a blood transfer conduit, and a manually operable pump unit (10) comprising first and second peristaltic pumps (10A, 10B). The first peristaltic pump is mounted about and acts upon the blood collection conduit. The second peristaltic pump is mounted about and acts upon the blood transfer conduit. The pump unit is provided with an actuator (112) adapted to switch the second peristaltic pump between an operative condition and an inoperative condition: in the operative condition the second peristaltic pump is engaged with the blood transfer conduit to convey blood from the blood collection reservoir while the first peristaltic pump is engaged with the blood collection conduit to convey blood from a wound site to the blood collection reservoir, and in the inoperative condition the second peristaltic pump is disengaged from the blood transfer conduit.

The invention discloses an autologous transfusion device of intestine contents after small intestine double-cavity coloproctia operation. The autologous transfusion device comprises an autologous transfusion device body which penetrates into a small intestine; the autologous transfusion device body comprises a pipe body made of an elastic material; a drop-proof air bag fixedly sleeves the external circumference of each of parts, close to two ends, of the pipe body; a plurality of annular first elastic bags and second elastic bags, which are fixed on an inner pipe wall, are arranged on the inner pipe wall of the pipe body along the axial direction; check valves are arranged between the first elastic bags and the second elastic bags; first connecting pipes penetrate into the first elastic bags; the first connecting pipes are provided with first branch pipes in cavities of the first elastic bags; second connecting pipes penetrate into the second elastic bags; the second connecting pipes are provided with second branch pipes in cavities of the second elastic bags. The autologous transfusion device disclosed by the invention has the following advantage that small intestine fluid, which is lost at a rear-end small intestine anastomosis and comprises digestive fluid and nutrient substances, can be recycled; and a far-end small intestine and colorectum recover functions as soon as possible and the atrophy of intestinal glands is reduced.

The invention relates to an autotransfusion machine. The autotransfusion machine consists of three parts of a machine body, casters and a support. The support is fixedly installed on the rear side end of the upper surface of the machine body; a display screen is positioned in a middle position on the upper surface of the machine body, an emergency switch and a power switch are fixed on the two sides of the display screen on the upper part of the machine body, and a peristaltic pump and a centrifuge cup are installed on the machine body in the front of the display screen; a material storage box is arranged on the lower part of the machine body, a lateral sliding rail is positioned above the material storage box on the front part of the machine body, and a suspension head is fixedly connected onto the lateral sliding rail and slides along the sliding rail left and right; and a safety power socket and a handle are arranged on the rear part of the machine body, and the casters are fixedly installed on the four corners of the bottom of the machine body and respectively provided with a self-lock device, so that the autotransfusion machine can be conveniently moved towards all directions in a use process. The autotransfusion machine is simple in structure and has multiple use functions; the angle of the display screen can be adjusted, so that the autotransfusion machine is convenient to operate and can quickly read data; a host automatically stores all the data so as to facilitate the checking in the operation or after operation; and the autotransfusion machine conveniently and quickly provides a great quantity of return blood for patients.

A method for recovering blood from a blood-laden surgical sponge for autologous reinfusion, the method comprising the steps of: conveying negative pressure to a housing with the blood-laden surgical sponge; applying a predetermined force to draw the blood from the surgical sponge; and collecting the recovered blood.

A method and system for treating, by filtration, haemorrhagic fluid contained in a container with a view to subsequent autotransfusion, including at least one step of concentrating by filtration said haemorrhagic fluid in order to increase the concentration of red blood cells in the haemorrhagic fluid to reach a target haematocrit level while at the same time removing from the haemorrhagic fluid a filtrate comprising compounds not desired for autotransfusion, further including:a preliminary step of measuring the haematocrit level of the haemorrhagic fluid; anda dilution step consisting in adding to the volume of haemorrhagic fluid to be treated a determined volume of dilution fluid, the determined volume of dilution fluid being calculated as a function of the measured haematocrit level of the haemorrhagic fluid and the target haematocrit level.

The invention relates to an establishing method of a pregnancy late trimester storing type autologous component blood transfusion system. A 300 ml sealing three-connecting-bag system is used. The anticoagulant capacity in a sealing three-connecting bag is normal capacity, 300 ml autologous blood is collected, the autologous blood is made into blood components in six hours, and the fact that a whole process is kept in a cold chain state is guaranteed. The blood components are suspension erythrocytes and frozen fresh plasma. A scientific basis is provided for establishing of the pregnancy late trimester storing type autologous blood transfusion standard, accordingly, storing type autologous blood transfusion can be well used in obstetrics patients, safety, effectiveness and economy are achieved, and therefore promotion and application of storing type autologous blood transfusion in obstetrics are well achieved.

A traditional method for treating IPF mainly comprises the step of taking anti-inflammatory drugs and anti-fibrosis drugs, and the anti-inflammatory drugs and the anti-fibrosis drugs are not ideal in curative effect on middle and advanced fibrosis patients. In recent years, new drugs pirfenidone and nintedanib approved in China can only delay the disease progress and cannot improve the lung function or reverse the disease progress. The invention develops a bronchus basal layer cell product, namely a REGEND001 cell autotransfusion preparation. In the early exploratory clinical research, through local administration of an airway, the medicine is transfused to the body of a patient with idiopathic pulmonary fibrosis in an autotransfusion mode, and the medicine has the effects of repairing lung injury and improving the lung ventilation function.

The invention provides a hand-pushed autologous transfusion blood collecting trolley, and belongs to the field of medical apparatus and instruments. The hand-pushed autologous transfusion blood collecting trolley is a medical blood collecting auxiliary device, is used for blood collection of hospital autologous transfusion, is mainly used for solving a problem that no special shaking device is provided in autologous transfusion blood collection in the prior art, is obtained via reasonable combination of a medical trolley and a shaking device, is provided with an appropriate power supply, is suitable to be applied in blood collection of hospital autologous transfusion, is convenient and reliable, and is capable of saving manpower and time, and reducing labour intensity.

The invention discloses a method for clinically improving breast cancer through TIL. The method comprises the steps that firstly, excising a tumor from the body of a patient; then transporting the tumor tissue to a laboratory under sterile and cold-chain conditions, locally separating tumor infiltrating lymphocytes from the tumor by using a mechanical treatment and enzyme digestion method, and separating to obtain initial tumor infiltrating lymphocytes; adding high-dose IL-2 for in-vitro culture and transformation, amplifying the initial tumor infiltration lymphocyte by ten thousand times, further purifying, and performing cryopreservation; finally, carrying out autotransfusion of the tumor infiltrating lymphocytes into the body of the biliary duct cancer patient, wherein the number of injected into the tumor infiltrating lymphocytes each time is 5 * 10 < 9 > to 5 * 10 < 10 >. According to the invention, lymphocytes in breast tumor tissues are separated and amplified, and then are fedback to the patient, so that the purpose of improving the living quality of the breast cancer patient can be achieved. The TIL tumor infiltration lymphocyte can recognize T cells of tumor cell neoantigens and has the capacity of efficiently killing tumor cells.